Browse > Article

Antioxidative and Anti-inflammatory Effects of Aurantii Fructus Immaturus Pharmacopuncture  

Kim, Sung-Jin (Dept. of Meridian & Acupoint, College of Oriental Medicine, Sangji University)
Park, Sang-Kyun (Dept. of Meridian & Acupoint, College of Oriental Medicine, Sangji University)
Publication Information
Korean Journal of Acupuncture / v.27, no.2, 2010 , pp. 13-24 More about this Journal
Abstract
Objectives : Ulcerative colitis is a chronic relapsing inflammatory disease in the gastrointestinal tract. We investigated whether Aurantii fructus immaturus (AFI) pharmacopuncture has antioxidative and anti-inflammatory effects. Methods : in vitro experiments, 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, superoxide dismutase (SOD) activity, prevention on $H_2O_2$-induced cell death in RAW264.7 cell line, DNA fragmentation, and cyclooxygenase-2 mRNA expression induced by lipopolysaccharide (LPS), were analyzed to investigate antioxidative and anti-inflammatory effect of AFI pharmacopuncture. in vivo experiment, a murine model of dextran sulfate sodium (DSS)-induced colitis was used to examine the effect of AFI pharmacopuncture on CV12 at different doses of 5 ${\mu}l$, 0.5 ${\mu}l$, 0.05 ${\mu}l$ for 10 days. Body weight, colon length and macroscopic features were investigated. Results : AFI pharmacopuncture showed DPPH free radical scavenging and SOD active effects in a dose-dependent manner. AFI pharmacopuncture showed a protective effect against $H_2O_2$-induced cell injury and also attenuated LPS-induced COX-2 mRNA expression. In a DSS- induced colitis murine model, however, AFI pharmacopuncture at CV12 had no anti-inflammatory effects. Conclusions : The present results suggest that AFI pharmacopuncture extract may have anti- inflammatory and antioxidative effects in vivo test, but further research on the underlying mechanism is required.
Keywords
Aurantii fructus immaturus; Antioxidative; Anti-inflammation; Dextran sulfate sodium; Inflammatory bowel disease;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Fiocchi C. Inflammatory bowel disease : dogma and heresies. Digest Liver Dis. 2002 ; 34 : 306-11.   DOI   ScienceOn
2 신성애, 김도순, 김미정, 김성호, 조성기, 변명우, 이성태. 과산화수소수의 산화적 스트레스로 유도된 Apoptosis에 대한 생약복합조성물(HemoHIM)의 방호효과 평가. J korean Soc Food Sci Nutr. 2006 ; 35(9) : 1127-32.   과학기술학회마을   DOI   ScienceOn
3 Nikolaus S, Schreiber S. Diagnostics of inflammatory bowel disease. Gastroenterology. 2007 ; 133 : 1670-89.   DOI   ScienceOn
4 Shin EK, Kwon HS, Kim YH, Shin HK, Kim JK. Vhrysin, a natural flavone, improves murine inflammatory bowel disease. Biochemical and Biophusical Research Communications. 2009 ; 318 : 502-7.
5 전국한의과대학 본초학교수. 본초학. 대성문화사. 1991 ; 351-2.
6 Rezaie A, Ghorbani F, Eshghtork A, Zamani MJ, Dehghan G, Taghavi B et al. Alteration in salivary antioxidantsm nitric oxide, and transformaing growth factor-bate 1 in relation to disease activity in Crohn's disease patients. Ann N Y Acd Sci. 2006 ; 1091 : 110-22.   DOI   ScienceOn
7 백설, 최재혁, 고성훈, 이용재, 차동석, 박은영, 강양규, 전훈. 감솜향의 in vitro 항산화 및 항염증 효과. 동의생리병리학회지. 2009 ; 23(4) : 853-9.   과학기술학회마을
8 나선택. Lonicerae folium의 항산화효과 탐색 및 RAW264.7 세포에서 항산화기전을 통한 산화적 스트레스.염증반응 억제효과 연구. 동국대학교 대학원. 2009.
9 진윤태, 김주형. 궤양성 대장염의 최신치료. 대한내과학회지. 2009 ; 76(6) : 654-60.
10 Silvio D, Erika A, Alberto M, Renzo C. Biological agents for Ulcerative Colitis : Hypes and Hopes. Medicinal Research Reviews. 2008 ; 28(2) : 201-18.   DOI   ScienceOn
11 고영은, 김미경, 조한영, 이인영, 이선영. Dextran sodium sulfate로 대장염을 유도한 흰쥐에서 캐피어 원말의 장보호 효과. 한국영양학회지. 2008 ; 41(5) : 391-401.   과학기술학회마을
12 최창환, 정성애, 이보인, 이강문, 김주성, 한동수, 대장장연구학회 IBD연구회. 궤양성 대장염 진단 가이드라인. 대한소화기학회지. 2009 ; 53 : 145-60.
13 양동훈, 양석균. 한국인에서 궤양성 대장염은 증가하는가?. 대한내과학회지. 2009 ; 76(6) : 637-42.
14 Lindermann RA, Economou JS. Actinobacillus actinomycetemcomitansand Bacteroides gingivalis activate human peripheral monocytes to produce interleukin-1 and tumor necrosis factor. J Periodontol. 1988 ; 59 : 728-30.   DOI
15 Kurt JI, Eugene B, Jean DW, Joseph BM, Anthony SF, Dennis LK. Harrison's Principles of Internal Medicien 13 edition. Mcgraw-hill, inc. 1994 : 1404.
16 Macpherson BR, Pfeiffer CJ. Experimental production of diffuse colitis in rat. Digestion. 1978 ; 17 : 135-50.   DOI   ScienceOn
17 Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL. Hepaten -induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989 ; 96 : 795-803.
18 Ishii K, Kanai T, Totsuka T, Uraushihara K, Ishikura T, Yamazaki M, Okamoto R, Araki A, Miyata T, Tezuka K, Nakamura T, Watanabe M. Hyperexpression of inducible co-stimulator on lamina propria mononuclear cells in rat dextran sulfate sodium colitis. Gastroenterology. 2004 ; 19(2) : 174-81.
19 Lindermann RA, Economou JS, Rothermel H. Production of interleukin-1 and tumor necrosis factor by human peripheral monocytes: Activated by peridontal bacteria and extracted lipopolysaccharides. J Dent Res. 1988 ; 67 : 1131-5.   DOI   ScienceOn
20 Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology. 1990 ; 98 : 694-702.
21 Dhar A, Young MR, Colburn NH. The role of AP-1, NF-kappa B and ROS/NOS in skin carcinogenesis : the JB6 model is predictive. Mol Cell Biochem. 2004 ; 234-235 : 185-93.
22 전국한의과대학, 한의학전문대학원 경락 경혈학 교재편찬위원회. 대학경락경혈학각론(하). 의방출판사. 2009 : 1172.
23 Kurz DJ, Decary S, Hong Y, Trivier E, Akhedov A, Erusalimsky JD. Chronic oxidative stress compromise telemere integrity and accelerates the onset of senscense in human endothelial cells. J Cell Sci. 2004 ; 117 : 2417-26.   DOI   ScienceOn
24 李時珍. 本草綱目. 文光圖書有限公司. 1992 : 1188-90.
25 Sjodin B, Westing HY, Apple FS. Biochemical mechanism for oxygen free radical formation during exercise. Sports Med. 1987 ; 10 : 236-54.
26 Berelt BS, Stadtman ER. Protein oxidation in aging, disease, and oxidative stress. J Biol Chem. 1997 ; 272 : 20313-6.   DOI   ScienceOn
27 許浚. 東醫寶鑑 제 5 권. 여강출판사. 1994 : 2813.
28 Satsangi J, Silverberg MS, Vermeire S, Colombel JF. The Montreal classification of inflammatory bowel disease : controversies, consensus and implications. Gut. 2006 ; 55: 749-53   DOI   ScienceOn
29 Fiocchi C. Inflammatory bowel disease : Etiology and pathogenesis. Gastroenterology. 1998 ; 115 : 182-205.   DOI   ScienceOn
30 Blois MS. Antioxidant determination by the use a stable free radicals. Nature. 1958 ; 26 : 1191-204.
31 Scaldaferri F, Fiocchi C. Inflammatory bowel disease:progress and current concepts of etiopathgenesis. J. Dig. Dis. 2007 ; 8 : 171-8   DOI   ScienceOn