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Analysis of Genetic Relationship Among Collected Cymbidium goeringii Based on RAPD  

Kim, Tae Bok (Jeollabuk-do Agricultural Research & Extension Services)
Lee, Jin Jae (Jeollabuk-do Agricultural Research & Extension Services)
Song, Young Ju (Jeollabuk-do Agricultural Research & Extension Services)
Choi, Chang Hak (Jeollabuk-do Agricultural Research & Extension Services)
Cheong, Dong Chun (Jeollabuk-do Agricultural Research & Extension Services)
Yu, Young Jin (Jeollabuk-do Agricultural Research & Extension Services)
Publication Information
FLOWER RESEARCH JOURNAL / v.19, no.4, 2011 , pp. 225-230 More about this Journal
Abstract
This research was performed for making data-base of cross-breeding between Cymbidium goeringii cultivars. Morphological characteristics were investigated and then genetic relationship was analyzed. Collected 20 Cymbidium goeringii cultivars were clustered into 2 groups. Seven cultivars were clustered into group I, and thirteen cultivars were clustered into group II. Group I doesn't have leaf pattern. Group have leaf pattern. The genetic relationship among collected 20 Cymbidium goeringii cultivars was anaylzed using RAPD with ten 10-mers random primer. Eighty-nine bands were generated by RAPD. Among the rest, three bands were monomorphic and eight-six bands were polymorphic. Overall similarity degree ranged from 0.521 to 0.862. The result of RAPD analysis was clustered into 2 groups, too. Sixteen cultivars were clustered into GroupX, and four cultivars were clustered into GroupY. Result of classification with morphological characteristics and RAPD showed different pattern, but 4 cultivars of GroupY by RAPD analysis were included in groupby morphological characteristics. Crossbreeding combination among low related coltivars in RAPD analysis may get more efficient result.
Keywords
crossbreeding; PCR; polymorphism; random primer; similarity;
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Times Cited By KSCI : 4  (Citation Analysis)
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