Browse > Article

Dormancy Breaking by Hot-water Treatment of Lilium Oriental Hybrids Bulblets Regenerated in Vitro  

Woo, Jin Ha (Gumi Floricultural Experiment Station, Gyeongbuk Agricultural Research and Extension Services)
Nam, Hyo Hoon (Gyeongbuk Agricultural Research and Extension Services)
Lee, Hyun Suk (Gumi Floricultural Experiment Station, Gyeongbuk Agricultural Research and Extension Services)
Choi, Dong Jin (Gumi Floricultural Experiment Station, Gyeongbuk Agricultural Research and Extension Services)
Oh, Wook (Department of Horticultural Science, Yeunganm University)
Kim, Kiu Weon (Department of Horticultural Science, Yeunganm University)
Publication Information
FLOWER RESEARCH JOURNAL / v.18, no.2, 2010 , pp. 93-97 More about this Journal
Abstract
The objective of this study was to investigate the effect of hot water and dipping times, and hot- PGR's solution on dormancy breaking of Lilium Oriental Hybrid 'Casa Blanca' and 'Siberia' bulblets regenerated in vitro. Hot-water treatments with distilled water were effective not sprouting rate but shortening of days to sprouting. While $100mg{\cdot}L^{-1}\;GA_{4+7}$ was effective in the dormancy breaking process of bulblets. The content of mitochondrial protein and fumarase activity were highest in bulblet of $100mg{\cdot}L^{-1}\;GA_{4+7}$ addition in hot water treatment. Therefore $GA_{4+7}$ addition in hot-water treatment seem to be the alternative dormancy breaking method to low-temperature treatment in Lilium Oriental Hybrid bulblet regenerated in vitro.
Keywords
fumarase; $GA_{4+7}$; mitochondrial protein;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Choi, S.T. 1983. Effects of low temperature and hot water treatment to the dormant bulbs on the leaf emergence of bulblets during scale propagation of Ester lily (Lilium longiflorum Thunb.). J. Kor. Soc. Hort. Sci. 24:42-48.
2 Juntilla, O. 1988. To be or not to be dormant: some comments on the new dormancy nomenclature. HortScience 23:805-860.
3 Kamerbeek, G.A., T.C.M. Beijersbergen, and P.K. Schenk. 1972. Dormancy in bulbs and corms. Proc. 18th Int. Hort. Congr. 5:233-239.
4 Kim, K.S. 1991. The effect of growth regulators, temperature and sucrose on the dormancy in Lilium speciosum bulblets cultured in vitro. Kor. J. Pant Tissue Culture. 18:103-111.
5 Kim, K.W. and A.A. De Hertogh. 1997. Tissue culture of ornamental flowering bulbs (geophytes). Hort Rev. 18:87-169.
6 Kim, K.W., J.S. Kim, E.Y. Kim, J.D. Choi, and K.I. Park. 1998. Effects of culture condition on dormant status of in vitro regenerated Lilium Oriental Hybrids bulblets. J. Kor. Soc. Hort. Sci. 39:641-646.
7 Kim, K.W., K.A. Kim, and M.S. Byun. 1999. Physiological changes before and after breaking dormancy of Lilium hansonii regenerated in vitro. J. Kor. Soc. Hort. Sci. 40:751-754.
8 Kim, K.W., S.Y. Moon, and S.J. Eum. 2003. Shortening dormancy breaking period by $GA_{4+7}$ addition in hot-water treatment of Korea native Lilium bulblets regenerated in vitro. J. Kor. Soc. Hort. Sci. 44:744-747.
9 Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.j. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193-275.
10 Niimi, Y., Y. Endo, and E. Arisaka. 1988. Effects of chilling and $GA_{3}$ treatments on breaking dormancy in Lilium rubellum Baker bulblets culture in vitro. J. Jpn. Soc. Hort. Sci. 57:250-257.   DOI
11 Moon, S.Y. 2002. Dormancy breaking by chilling and hot-water treatment of Lilium davuricum and L. hansonii bulblets regenerated in vitro. MS Thesis. Yeungnam University. Geyeongsan, Korea.
12 Racker, E. 1950. Spectrophotometric measurements of the enzymatic formation of fumaric acid cis-aconitic acid. Bioochem. Biophys. Acta. 4:211-214.   DOI   ScienceOn
13 Roh, S.M. and H.F. Wilkins. 1976. The physiology of dormancy and maturity of Lilium longiflorum Thunb. cv. Nellie White bulb. J. Kor. Soc. Hort. Sci. 17:173-179.
14 Woo, J.H., Y.G. Sim, Y.Y. Han, H.H. Nam, K.B. Choi, and K.W. Kim. 2001. Effect of culture medium and it's physico-chemical properties on bulblet growth of Lilium Oriental Hybrid. J. Kor. Soc. Hort. Sci. 42:465-468.
15 Stimart, D.P. and D.P. Ascher. 1981. Developmental responses of Lilium longiflorum bulblets to constant or alternating temperature in vitro. J. Amer. Soc. Hort. Sci. 106:450-454.
16 Stimart, D.P., D.P. Ascher, and H.F. Wilkins. 1982. Overcoming dormancy in Lilium longiflorum bulblets produced in tissue culture. J. Amer. Soc. Hort. Sci. 107:1004-1007.
17 Woo, J.H., H.H. Nam, K.B. Choi, I.S. Park, and K.W. Kim. 2003. Dormancy and culture temperature of Lilium Oriental Hybrid bulblets regenerated in vitro. J. Kor. Flower Res. Soc. 11:143-147.