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http://dx.doi.org/10.18325/jkmr.2022.32.4.9

Anti-inflammatory Effects of Naetakbaekryeom-san  

Jung, Min Jae (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Noh, Hui Jeong (Department of Korean Medicine, Rehabilitation, Dongshin University Mokpo Korean Medicine Hospital)
Choi, Ji Min (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Jeon, Seok Hee (Department of Acupuncture and Moxibustion Medicine, Dongshin University Mokpo Korean Medicine Hospital)
Kim, Seon Jong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.32, no.4, 2022 , pp. 9-18 More about this Journal
Abstract
Objectives This study was conducted to confirm the anti-inflammatory effect of Naetakbaekryeom-san (NTB), and whether it could be another treatment for inflammatory diseases. Methods The NTB water extract was extracted with hot water at 100℃ for 2 hours, concentrated at 80℃ under reduced pressure, and used. After 2 hours of pretreatment with NTB and positive control Bay11-7082, nitric oxide (NO), inducible NO synthase (iNOS), interleukin (IL)-6, IL-1𝛽, tumor necrosis factor alpha (TNF-𝛼) were measured in RAW264.7 cells activated with lipopolysaccharides (LPS) 500 ng/mL. After 2 hours of pretreatment with NTB, the anti-inflammatory effect of NTB was evaluated by measuring nuclear factor kappa-light-chain-enhancer of activated B cells (NF-𝜅B) in RAW264.7 cells and 293T cells activated with phorbol 12-myristate 13-acetic acid (PMA) 30 ng/mL. Results In RAW264.7 cells activated with LPS, NTB at concentrations of 0.1, 0.3, and 1.0 mg/mL showed no cytotoxicity, significantly inhibited NO production and inhibition of iNOS expression. TNF-𝛼 cytokine levels was not regulated, but NTB at each concentration inhibited the production of IL-1𝛽 and IL-6, and the effect was higher than that of the positive control Bay11-7082 (20 𝜇M). In PMA-activated RAW264.7 cells and 293T cells, each concentration of NBT decreased the NF-𝜅B transcriptional activity, with the greatest decrease at 1 mg/mL. Conclusions These results demonstrated the anti-inflammatory effect of NTB water extracts, but further studies such as comparison of anti-inflammatory effects and antioxidant effects by NTB component, comparison of effects according to extraction solvents, and clinical studies are needed.
Keywords
Naetakbaekryeom-san; Anti-inflammatory; Herbal medicine;
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