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http://dx.doi.org/10.18325/jkmr.2021.31.4.1

Anti-neuroinflammatory Effects of Hwanggeumjakyak-tang on Lipopolysaccharide-induced Brain Injury Model in vivo and in vitro  

Kim, Jong-gyu (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University)
Im, Ji-sung (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Wonkwang University)
An, Sung-Hu (Department of Korean Medicine Rehabilitation, Gwangju Medical Center, College of Korean Medicine, Wonkwang University)
Song, Yung-sun (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.31, no.4, 2021 , pp. 1-11 More about this Journal
Abstract
Objectives Hwanggeumjakyak-tang (HJT) has traditionally been used to treat gastrointestinal inflammatory diseases; however, its protective effects against neuronal inflammation are still undiscovered. Methods We investigated the anti-neuroinflammatory effects of HJT water extract on lipopolysaccharide (LPS)-stimulated BV2 mouse microglia cells. BV2 cells were treated with LPS (1 ㎍/mL) 1 hour prior to the addition of HJT. We measured cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and nitrite production using the Griess assay. We performed a reverse transcription-polymerase chain reaction assay to measure messenger RNA expression of inflammatory cytokines including interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α. Western blot analysis was performed to determine protein expression of mitogen-activated protein kinases (MAPKs) and inhibitor of nuclear factor kappa B (NF-κB)α. Results HJT inhibited excessive nitrite release in LPS-stimulated BV2 cells and also significantly inhibited inflammatory cytokines such as IL-1β, IL-6, and TNF-α in LPS-stimulated BV2 cells. Moreover, HJT significantly suppressed LPS-induced MAPK and NF-κB activation and inhibited the elevation of IL-1β, IL-6, and TNF-α in the brain of LPS-injected mice. Conclusions Our study highlights the anti-neuroinflammatory effects of HJT via MAPK and NF-κB deactivation.
Keywords
Hwanggeumjakyak-tang (HJT); Anti-neuroinflammatory effect; Neuroprotective agents; Pro-inflammatory cytokines Microglia cell;
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