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http://dx.doi.org/10.18325/jkmr.2021.31.1.33

Anti-Arthritic Effect of Sogyunghwalhyel-tang-gamibang  

Jo, Joo-hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University)
Im, Ji-sung (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University)
Kim, Jong-gyu (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University)
Park, Jung-hyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Wonkwang University)
Choi, Hag-soon (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Hwang, Geu-won (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Song, Yung-sun (Department of Third Medicine of Korean Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.31, no.1, 2021 , pp. 33-46 More about this Journal
Abstract
Objectives The aim of this study is to evaluate anti-inflammatory and anti-arthritic effects of Sogyunghwalhyel-tang-gamibang (SGHHTGB) in cell and animal models and also to suggest one of putative mechanisms underlying its anti-arthritic effects. Methods Enzyme-linked immunosorbent assay was applied to measure the concentrations of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E2 (PGE2) in culture medium and blood serum and nitric oxide (NO) was assayed by Griess reagent. The expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) were analyzed by Western blot method. Results In a cell model using RAW264.7 macrophages stimulated with the endotoxin lipopolysaccharide (LPS), the drug, at its non-cytotoxic concentrations, inhibited the production of the pro-inflammatory cytokine TNF-α, IL-1β and IL-6. In addition, it suppressed the expression of the inflammatory enzyme iNOS and COX-2, and reduced the synthesis of the enzyme product NO (as stable nitrite) and PGE2 in activated macrophages. Meanwhile, in an animal model using rheumatic arthritis (RA) mice induced with injection of type II collagen antibody (CAb) and LPS, the drug improved clinical symptom of arthritis and reduced paw thickness and inflammatory cell infiltration. In blood of RA mice, the drug reduced serum levels of TNF-α, IL-1β, IL-6, nitrite, and PGE2, all inflammatory mediators produced by activated macrophages. Conclusions SGHHTGB may ameliorate CAb and LPS-induced RA in mice, presumably by inactivating macrophages that are capable of initiating joint inflammation by producing pro-inflammatory cytokines and expressing inflammatory enzymes.
Keywords
Sogyunghwalhyel-tang-gamibang; Rheumatoid arthritis; Anti-arthritic effect; Pro-inflammatory cytokine; Macrophages;
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