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Study of Mori Fructus and Dried Mori Fructus Extracts on the Antioxidant Effect and the Inhibitory Effect on Adipocyte Differentiation  

Kim, Hyung-Gu (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Wang, Jing-Hua (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Lim, Dong-Woo (Departments of Pathology, College of Oriental Medicine, Dongguk University)
Chae, Hee-Sung (College of Pharmacy, Dongguk University)
Chin, Young-Won (College of Pharmacy, Dongguk University)
Choi, Han-Seok (Departments of Endocrinology Medicine, College of Medicine, Dongguk University)
Kim, Hojun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.24, no.4, 2014 , pp. 1-13 More about this Journal
Abstract
Objectives This study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells. Methods 3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including $C/EBP-{\alpha}$, $C/EBP-{\beta}$, $PPAR-{\alpha}$, $PPAR-{\gamma}$, $AMPK-{\alpha}$ were analyzed by Real time PCR. Results Mori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructus extracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of $C/EBP-{\alpha}$, $C/EBP-{\beta}$ genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of $PPAR-{\alpha}$, $PPAR-{\gamma}$, $AMPK-{\alpha}$ genes. Conclusions Mori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes.
Keywords
Mori Fructus; Dried Mori Fructus; Antioxidative; Antiobesity; Adipocyte differentiation;
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Times Cited By KSCI : 4  (Citation Analysis)
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