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Anti-inflammatory Effect of Mugi-hwan Water Extract in RAW 264.7 Cells  

Kim, Ilhyun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Choi, Chonghwan (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Lee, Sewon (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Song, Yungsun (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.23, no.3, 2013 , pp. 27-35 More about this Journal
Abstract
Objectives The aim of this study was to investigate anti-inflammatory activity of Mugi-hwan (MGH) Water Extract. Methods Cells were treated with 2 ug/ml of LPS 1 hour prior to the addition of MGH. Cell viability was measured by MTS assay. The production of NO was determined by reacting cultured medium with Griess reagent. The expression of COX-2, iNOS and MAPKs was investigated by Western blot, RT-PCR. The content of level of cytokines ($PGE_2$, IL-6, in media from LPS-stimulated Raw 264.7 cells was analyed by ELISA kit. Results MGH inhibited the production of NO, $PGE_2$, IL-6 as well as the expressions of iNOS, COX-2 in the murine macrophage, RAW 264.7 cells. MGH also had suppression effects of LPS induced MAPKs activation. Conclusions These results suggest that MGH has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.
Keywords
Mugi-hwan; MAPKs; Anti-inflammatory activity;
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