Browse > Article

The Effect of Bee Venom Pharmacopuncture Therapy on the Condition of Different Concentration in Rheumatoid Arthritis Rat Model  

You, Deok-Seon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Yeom, Seung-Ryong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Lee, Su-Kyung (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Kwon, Young-Dal (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Song, Yung-Sun (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Won-Kwang University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.21, no.2, 2011 , pp. 101-123 More about this Journal
Abstract
Objectives : The aim was to study the effect of bee venom pharmacopuncture therapy with different concentration on rheumatoid arthritis rat model. Methods : We enforced a bee venom pharmacopuncture therapy with different concentration on rheumatoid arthritis rat model by the intradermal injection of chicken type II collagen emulsified. 14 days after the onset of the rheumatoid arthritis rat model, a fixed volume of bee venom was daily injected to ST-35 acupoint in the rat's knee joint for 2-3 weeks. The hind paw volume, arthritic index, arthritic flexion pain test, pain threshold, and serum analysis (CRP, $PGE_2$, ALT, AST) were analyzed, and the expression profiles of COX-2, c-fos, and substance-P at the dorsal horn region of the spinal cord and subchondral bone of the knee joint were also analyzed by using the immunohistochemistry. Results : After the treatment of rheumatoid arthritis rats with bee venom pharmacopuncture, the paw volume of edema of arthritic rats were almost restored to the level of normal group, and behavior tests were very effective. Also the evaluation on the blood serum analysis was remarkable. COX-2, c-fos, and substance-P positive cells in the immunohistological section of dorsal horn region of the spinal cord and subchondral bone of the knee joints were significantly decreased. also the bee venom pharmacopuncture was effective to alleviate their rheumatoid arthritic inflammation cytokine inhibition as regards to the behavior tests and joint histological appearance. Conclusions : Based on the results in this study, bee venom pharmacopuncture with concentrated treatment condition was very effective in low fixed quantity and progressive low increased quantity.
Keywords
Bee venom pharmacopuncture therapy; Concentrated treatment condition; Rheumatoid arthritis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Mark H. Swartz. 슈왈츠 임상진단학. 정담. 2004:613-4.
2 한방재활의학과학회. 한방재활의학. 군자출판사. 2005:97.
3 대한가정의학회. 최신가정의학. 한국의학. 2007:1312.
4 Sweeney SE, Firestein GS. Rheumatoid arthritis: regulation of synovial inflammation. Int Journal Biochem Cell Biol. 2004;36(3):372-8.   DOI   ScienceOn
5 함상근. 류마티스 관절염의 약물치료. 가정의학회지. 2008;29:320-3.
6 권재식, 김기현, 김형태, 박응호, 서성훈. 비중. 서울:정담. 1993:29, 208-10, 214.
7 김무진, 윤종화, 김경호, 이승덕, 김갑성. 류마티스성 膝關節炎의 鍼灸治療에 관한 문헌고찰. 대한침구학회지. 2005;22(1):191-201.
8 이재동. 류마티스 관절염의 한방치료. 한방척추관절학회지. 2007;4(1):43-8.
9 대한약침학회. 약침요법시술지침서. 대한약침학회. 1999:133-5.
10 朱文鋒. 實用中醫辭典. 陜四:陜四科學技術出版社. 1982:402.
11 대한약침학회. 약침요법총론. 대한약침학회. 1999:262, 264.
12 Habermann E. Chemistry, pharmacology and toxicology of bee, wasp and hornet venoms. in venomous animals and their venoms. Academic Press. 1971;3:61.
13 Assem ES, Atkinson G. Histamine release by MCDP (401), a peptide from the venom of the honey bee. Brit Pharmacol. 1973;337-8.
14 권기록. 蜂針에 대한 考察. 대한침구학회지. 1994;11(1):160.
15 인창식, 고형균. 봉독요법에 대한 한의학 최초의 문헌기록 마왕퇴의서의 봉독요법 2례. 대한침구학회지. 1998;15(1):143.
16 Lee JD, Park HJ, Chae YB, Lim S. An Overview of bee venom acupuncture in the treatment of arthritis. eCAM. 2005;2(1):79-84.
17 권기록, 고형균, 김용석, 박영배, 김창환, 강성길. 봉독약침자극이 3-MCA 유발 상피종에 대한 항암 및 면역반응에 미치는 영향. 대한침구학회지. 1997;14(2):151-72.
18 이성노, 홍서영, 조현철, 변임정, 송호섭, 김기현. 봉약침치료의 퇴행성슬관절염에 대한 임상적 고찰. 대한침구학회지. 2003;20(5):73-8.
19 원중희, 최의성, 홍성선. 골관절염 환자에서 봉독 주사액의 효과. 대한류마티스학회지. 1999;6(3):218-26.
20 전형준, 황욱, 김정신, 남성수, 김용석. 봉약침으로 치료한 요추간판탈출증 환자의 임상적 평가. 대한침구학회지. 2003;20(5):63-72.
21 강계성, 권기록. 다발성 경화증 환자 5례에 대한 임상보고. 대한침구학회지. 2003;20(1):209-17.
22 권기록. 한방치료를 통한 근위축성 측상경화증의 임상적 연구. 대한침구학회지. 2003;20(3):209-16.
23 이상훈, 이현종, 백용현, 김수영, 박재경, 홍승재, 양형인, 김건식, 이재동, 최도영, 이두익. 봉독약침이 류마티스 관절염 환자의 관절 통증, 종창 및 급성염증 반응에 미치는 영향. 대한침구학회지. 2003;20(2):77-84.
24 이상훈, 홍승재, 김수영, 양형인, 이재동, 최도영, 아두익, 이윤호. 무작위 대조 이중맹검 시험을 통한 봉독 약침의 류마티스 관절염 치료 효과 연구. 대한침구학회지. 2003;20(6):80-8.
25 Moon DO, Park SY, Lee KJ, Heo MS, Kim KC. Kim MO, Lee JD, Choi YH, Kim GY. Bee venom and melittin reduce proinflammatory mediators in lipopolysaccharide-stimulated BV2 microglia. Int Immunopharmacol. 2007 Aug;7(8):1092-101.   DOI   ScienceOn
26 Kim KW, Shin YS, Kim KS, Chang YC, Park KK. Park JB, Choe JY, Lee KG, Kang MS, Park YG, Kim CH. Suppressive effects of bee venom on the immune responses in collagen-induced arthritis in rats. Phytomedicine. 2008 Dec;15(12):1099-107.   DOI   ScienceOn
27 전형준, 김용석. MPTP로 유발된 파킨슨 병 mouse모델에 대한 봉약침의 농도의존적 효과. 대한침구학회지. 2010;27(5):59-68.
28 Department of health, education, and welfare publication(National institute of health). Guide for the care and use of laboratory animals. Washington, D.C.:National Academy Press. 1985:85-123.
29 Matsuura M, Imayoshi T, Okumoto T. Effect of FTY720, a novel immunosuppressant, on adjuant and collagen-induced arthritis in rats. Int Journal Imm. 2000;22:323-31.
30 Chen Q, Wei W. Effects mechanisms of glucosides of chaenomeles speciosa on collagen-induced arthritis in rats. Int Immunopharmacol. 2003;3(4):593-608.   DOI   ScienceOn
31 Winter CA, Risley EA, Nuss GW. Anti-inflammatory and antipyretic activities of indomethacin, 1-(p-chlorobenzoyl1)-5-methoxy-2-methylindole-3-acetic acid. J Pharmacol Exp Ther. 1963;141:369-76.
32 Burrai I, Henderson B, Knight SC, Staines NA. Suppression of collagen type II induced arthritis by transfer of lymphoid cells from rats immunized with collagen. Clin Exp Immunol. 1985;61:368-72.
33 Liu HX, Tian JB, Luo F, Jiang YH, Deng ZG, Xiong L, Liu C, Wang JS, Han JS. Repeated 100 Hz TENS for the treatment of chronic inflammatory hyperalgesia and suppression of spinal release of substance P in monoarthritic rats. Ecid Based Complement Alternat Med. 2007;4(1):65-75.   DOI   ScienceOn
34 Shimizu I, Iida T, Guan Y, Zhao C, Raja SN, Jarvis MF, Cockayne DA, Caterina MJ. Enhanced thermal avoidance in mice lacking the ATP receptor P2X3. Pain. 2005;116(1-2):96-108.   DOI   ScienceOn
35 Sluka KA, Chandran P. Enhanced reduction in hyperalgesia by combined administration of clonidine and tens. Pain. 2002;100(1-2):183-90.   DOI   ScienceOn
36 Mankin HJ. Biochemical and metabolic aspects of osteoarthritis. Orthop Clin North Am. 1971Mar;2(1):19-31.
37 Galois L, Etienne S, Grossin L, Watrin-Pinzano A, Cournil-Henrionnet C, Loeuille D, Netter P, Mainard D, Gillet P. Dose-response relationship for exercise on severity of experimental osteoarthritis in rats: a pilot study. Osteoarthritis Cartilage. 2004;12(10):779-86.   DOI   ScienceOn
38 Smolen JS, Aletaha D. The assessment of disease activity in rheumatoid arthritis. Clin Exp Rheumatol. 2010May-Jun;28(3 Suppl 59):18-27.
39 Weyand CM, Bryl E, Goronzy JJ. The role of T cells in rheumatoid arthritis. Warsz:Arch Immunol Ther Exp. 2000;48(5):429-35.
40 한태륜, 방문석 외. 재활의학. 군자출판사. 2008:908, 914.
41 김윤정. 한국인 류마티스 관절염의 역학, 임상 연구. 한양대학교 대학원. 2010.
42 Scott DL, Wolfe F, Huizinga TW. Rheumatoid arthritis. Lancet. 2010Sep25;376(9746):1094-108.   DOI   ScienceOn
43 Ridderstad A, Abedi-Valugerdi M, Moller E. Cytokines in rheumatoid arthritis. Ann Med. 1991Aug;23(3):219-23.   DOI   ScienceOn
44 Robak T, Gladalska A. Cytokines in rheumatoid arthritis. Postepy Hig Med Dosw. 1997;51(6):621-36.
45 대한침구학회 교재편찬위원회. 침구학 중. 파주:집문당. 2008:429, 431.
46 Ziai MR, Russek S, Wang HC, Beer B, Blume AJ. Mast cell degranulating peptide: a multifunctional neurotoxin. J Pharm Pharmacol. 1990;42:457-61.   DOI
47 Hider RC. Honeybee venom: a rich source of pharmacologically active peptides. Endeavour. 1988;12:60-5.   DOI   ScienceOn
48 박형규, 하지용, 안규석. 蜂毒(APITOXIN)이 흰 쥐의 Adjuvant 關節炎에 미치는 影響. 동의생리병리학회지. 1993;8(1):17-28.
49 Schaible HG, Grubb BD. Afferent and spinal mechanisms of joint pain. Pain. 1993Oct;55(1):5-54.   DOI   ScienceOn
50 Surh YJ, Chum KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res. 2001Sep;1(480-481):243-68.
51 Chen SH, Fahmi H, Shi Q, Benderdour M. Regulation of microsomal prostaglandin E2 synthase-1 and 5-lipoxygenase-activating protein/5-lipoxygenase by 4-hydroxynonenal in human osteoarthritic chondrocytes. Arthritis Res Ther. 2010;12(1):R21. Epub 2010 Feb 9.   DOI   ScienceOn
52 Neidel J, Schulze M, Lindschau J. Association between degree of bone-erosion and synovial fluid-levels of tumor necrosis factor alpha in the knee-joints of patients with rheumatoid arthritis. Inflamm Res. 1995May;44(5):217-21.   DOI   ScienceOn
53 Neidel J, Schulze M, Lindschau J. Association between degree of bone-erosion and synovial fluid-levels of tumor necrosis factor alpha in the knee-joints of patients with rheumatoid arthritis. Inflamm Res. 1995May;44(5):217-21.   DOI   ScienceOn
54 O'Dell JR. Therapeutic strategies for rheumatoid arthritis. N Engl J Med. 2004Jun17;350(25):2591-602.   DOI   ScienceOn