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Effects of Root of Cibotii Rhizoma on Neuronal Damage of Spinal Cord Contusion Injury in Rats  

Park, Won-Sang (Dept. of Oriental Rehabiiltation Medicine, Ja-Seng Hospital of Oriental Medicine)
Kim, Eun-Seok (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Shin, Jung-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Kim, Bum-Hoi (Dept. of Anatomy, College of Oriental Medicine, Dong-Eui University)
Kim, Seong-Joon (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Kang, Hee (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Sohn, Nak-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
Publication Information
Journal of Korean Medicine Rehabilitation / v.20, no.2, 2010 , pp. 1-15 More about this Journal
Abstract
Objectives : This study was performed to evaluate the effects of root of Cibotii rhizoma(CR) ethanol extract on the tissue and neuronal damage of the spinal cord injury(SCI). Methods : SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. CR was orally given once a day for 7 days after SCI. Tissue damage and nerve fiber degeneration were examined with cresyl violet and luxol fast blue(LFS) histochemistry. HSP72(as neuronal damage marker), MAP2(as nerve fiber degeneration marker), c-Fos(immediate early gene), and Bax(pro-apoptotic molecule) expressions were examined using immuno-histochemistry. Individual immuno-positive cells expressing HSP72, MAP2, c-Fos and Bax were observed on the damaged level and the upper thoracic and lower lumbar spinal segments. Results : 1. CR reduced degeneration of nerve fibers and motor neuron shrinkage in the ventral horn of the lower lumbar spinal segment, but generally it did not seem to ameliorate the tissue injury following SCI. 2. CR reduced demyelination in the ventral and lateral funiculus of the lower lumbar spinal segment. 3. CR reduced HSP72 expression on the neurons in the peri-central canal gray matter adjacent to the damaged region. 4. CR strengthened MAP2 expression on the motor neurons in the ventral horn and on nerve fibers in the lateral funiculus of the lower lumbar spinal segment. 5. CR reduced c-Fos positive cells in the peri-lesion and the dorsal horn of the damaged level and in the ventral horn of the lower lumbar spinal segment. 6. CR reduced Bax positive cells in the peri-lesion and the dorsal horn of the damaged level and in the ventral horn of the lower lumbar spinal segment. Conclusions : These results suggest that CR plays an inhibitory role against secondary neuronal damage and nerve fiber degeneration. following SCI.
Keywords
Cibotii Rhizoma; Spinal cord injury; HSP72; MAP2; c-Fos; Bax;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Farah CA, Liazoghli D, Perreault S, Desjardins M, Guimont A, Anton A, et al. Interaction of microtubule-associated protein-2 and p63: a new link between microtubules and rough endoplasmic reticulum rrembranes in neurons. J Biol Chem. 2005;280:9439-49.   DOI
2 Akins PT, Liu PK, Hsu CY. Immediate early gene expression in response to cerebral ischemia: friend or foe? Stroke. 1996;27:1682-7.   DOI   ScienceOn
3 Gross A, McDonnell JM, Korsmeyer SJ. Bcl-2 family members and the mitochondria in apoptosis. Genes Develop. 1999;13:1899-911.   DOI   ScienceOn
4 Crowe MJ, Bresnahan JC, Shuman SL, Masters JN, Beattie MS. Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nat Med. 1997;3:73-6.   DOI   ScienceOn
5 Emery E, Aldana P, Bunge MB, Puckett W, Srinivasan A, Keane RW, et al. Apoptosis after traumatic human spinal cord injury. J Neurosurg. 1998;89:911-20.   DOI
6 Yang C, Arnold PM, Zoubine MN, Citron BA, Watanabe I, Berman NE, et al. Apoptosis in cellular compartments of rat spinal cord after severe contusion injury. J Neurotrauma. 1998;15:459-72.   DOI   ScienceOn
7 Ackery A,Tator C,Krassioukov A.A global perspective on spinal cordinjury epidemiology. J Neurotrauma.2004;21:1355-70.   DOI   ScienceOn
8 Wyndaele M,Wyndaele JJ. Incidence, prevalence and epidemiology of spinalcordinjury: what learns a orldwide literature survey? Spinal Cord.2006;44:523-9.   DOI   ScienceOn
9 DeVivo MJ. Causes and costs of spinal cord injury in the United States. Spinal Cord.1997;35:809-13.   DOI   ScienceOn
10 Barnabe-Heider F,Frisen J. Stem cells for spinal cord repair. Cell Stem Cell.2008;3:16-24   DOI   ScienceOn
11 Deumens R, Joosten EA, Waxman SG, Hains BC. Locomotor dysfunction and pain: the scylla and charybdis of fiber sprouting after spinal cordinjury. Mol Neurobiol. 2008;37:52-63   DOI   ScienceOn
12 Kalous A, Osborne PB, Keast JR. Acute and chronic changes in dorsal horn innervation by primary afferents and descending supraspinal pathways after spinal cord injury. J Comp Neural. 2007;504:238-53.   DOI   ScienceOn
13 Beattie MS, Hermann GE, Rogers RC, Bresnahan JC, Cell death in models of spinal cord injury. Frog Brain Res. 2002;137:37-47.   DOI
14 정춘근, 김은영, 신정원, 손영주, 이현삼, 정혁상 외 청폐사간탕이 당뇨유발 흰쥐의 뇌허혈 손상에 미치는 영향. 대한한의학회지 2005;26 217-30   과학기술학회마을
15 강승준, 금현수, 전연이, 이은주, 박치상, 박창국. 석창포가 뇌허혈을 유발시킨 백서에서의 뇌신경보호효과. 대한한방내과학회지 2001;22 341-51
16 강봉주, 조동욱, 홍성길. 저산소상태에서 육미지황원의 뇌신경세포 보호효과에 대한 연구. 한국한의학연구원논문집. 2001;7:115-24
17 이상인. 본초학. 서울:의약사. 1975:97-8
18 김호철. 한약약리학. 서울:집문당. 2001:451.
19 김영호, 이용태. 난소적출로 유도된 흰쥐의 골다공증에 미치는 구척의 효능. 동의생리학회지 1995;10:17-29
20 심상도, 김호철, 이상인, 안덕균. 구척이 난소 적출로 유발한 흰쥐의 골다공증에 미치는 영향. 경희한의대논문집. 1995;24:377-86
21 김형균, 김종훈. 구척이 흰쥐의 후지체강에 의한 근위축에 미치는 영향. 대한한방내과학회지. 1996;17:51-65
22 임강현, 김호철. 구척이 흰쥐의 장골 길이성 장에 미치는 영향. 대한본초학회지. 2001;16:49-55
23 암강현, 김명규, 김호현, 박선영, 이동환, 부영민 외. 구척 분획물의 성장기 흰쥐 장골길이 성장에 대한 효과. 대한본초학회지. 2003;18:153-9   과학기술학회마을
24 김상태, 한용남, 손연경, 장형석, 김수장, 신준식. 구척으로부터 신경재생 효능 성분 분리. 약학회지. 2002;46:398-404   과학기술학회마을
25 김상태, 김정도, 김영균. 구척 메탄올추출액이 신경세포의 재생 및 회복효과에 미치는 영향. 생약학회지. 2004;35:105-9   과학기술학회마을
26 Louro J, Pearse DD. Stem and progenitor cell therapies:recent progress for spinal cord injury repair. Neurol Res. 2008;30:5-16   DOI   ScienceOn
27 Bunge MB. Novel combination strategies to repair the injured mammalian spinalcord. J Spinal Cord Med. 2008;31:262-9.   DOI
28 Schwab JM, Brechtel K, Mueller CA, Failli V, Kaps HP,Tuli SK, et al. Experimental strategies to promote spinal cord regeneration-anintegrative perspective. Prog Neurobiol. 2006;78:91-116   DOI   ScienceOn
29 Leem K, Boo Y, Park SY, Lee DH, Kim H. Effect of Cibotii rhizoma on the growfh hormone secretion of aldolescent male rats. Kor J Herbology. 2002;17:133-8.   과학기술학회마을
30 Young W. Spinal cord contusion models. Frog Brain Res. 2002;137:231-55.   DOI
31 Tator CH. Biology of neurological recovery and functional restoration after spinal cord injury. Neurosurgery. 1998;42:696-707.   DOI   ScienceOn
32 Tator CH. Update on the pathophysiology and pathology of acute spinal cord injury. Brain Pathol. 1995;5:407-13.   DOI
33 Stokes BT, Jakeman LB. Experirrental modelling of human spinal cord injury: a model that crosses the species barrier and mimics the spectrum of human cytopathology. Spinal Cord. 2002;40:101-9.   DOI   ScienceOn
34 Graham DI, McIntosh TK, Maxwell WL, Nicoll JAR. Recent advances in neurotrauma. J Neuropafhol Exp Neural. 2000;59:641-51.   DOI
35 Metz GA, Curt A, van de Meent H, Klusman I, Schwab ME, Dietz V. Validation of fhe weight-drop contusion model in rats: a comparative study of human spinal cord injury. J Neurotrauma. 2000;17:1-17.   DOI   ScienceOn
36 Bunge RP, Puckett WR, Becerra JL., Marcillo A, Quencer RM Cbservations on the pathology of human spinal cord injury. A review and classification of 22 new cases with details from a case of chronic cord compression "With extensive focal demyelination. Adv Neural. 1993;59:75-89.
37 Kakulas BA A review of the neuropathology of human spinal cord injury with emphasis on special features. J Spinal Cord Med. 1999; 22:119-24.   DOI
38 Fawcett JW, Asher RA. The glial scar and central nervous system repair. Brain Res Bull. 1999;49:377-91.   DOI   ScienceOn
39 Grimpe B, Silver J. The extracellular matrix in axon regeneration. Frog Brain Res. 2002 ;137:333-49.   DOI
40 Blesch A, Tuszynski MH. Spinal cord injury: plasticity, regeneration and the challenge of translational drug development. Trends Neurosci. 2009;32:41-7.   DOI   ScienceOn
41 Scholtz CL. Quantitative histochemistry of myelin using Luxol Fast Blue MBS. Histochem J. 1977;9:759-65.   DOI   ScienceOn
42 Lindquist S. Heat-shock proteins and stress tolerance in microorganisms. Curr Opin Genet Dev. 1992;2:748-55.   DOI   ScienceOn
43 Welch WJ. Heat shock proteins functioning as molecular chaperones: their roles in normal and stressed cells. Philos Trans R Soc Land B Biol Sci. 1993;339:327-33.   DOI   ScienceOn
44 Weinstein PR, Hong S, Sharp FR. Molecular identification of the ischemic penumbra. Stroke. 2004;35:2666-70.   DOI   ScienceOn
45 Zhang SX, Underwood M, Landfield A, Huang FF, Gison S, Geddes JW. Cytoskeletal disruption following contusion injury to the rat spinal cord. J Neuropathol Exp Neurol. 2000;59:287-96.   DOI
46 Li GL, Farooque M, Lewen A, Lennmyr F, Holtz A, Olsson Y. MAP2 and neurogranin as markers for dendritic lesions in CNS injury. An immunohistochemical study in the rat. APMIS. 2000;108:98-106.   DOI   ScienceOn
47 Gonzalez SL, Lopez-Costa JJ, Labombarda F, Deniselle MC, Guennoun R, Schumacher M, et al. Progesterone effects on neuronal ultrastructure and expression of microtubule -associated protein 2(MAP2) in rats with acute spinal cord injury. Cell Mol Neurobiol. 2009;29:27-39.   DOI   ScienceOn
48 Kikuchi H, Doh-ura K, Kawashima T, Kira J, Iwaki T. Immunohistochemical analysis of spinal cord lesions in amyotrophic lateral sclerosis using microtubule-associated protein 2(MAP2) antibodies. Acta Neuropathol. 1999;97:13-21.   DOI   ScienceOn