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http://dx.doi.org/10.9713/kcer.2021.59.3.366

Force Transmission in Cellular Adherens Junction Visualized by Engineered FRET Alpha-catenin Sensor  

Jang, Yoon-Kwan (Department of Integrated Biological Science, Pusan National University)
Suh, Jung-Soo (Department of Integrated Biological Science, Pusan National University)
Suk, Myungeun (Department of Mechanical Engineering, IT Convergence College of Components and Materials Engineering, Dong-Eui University)
Kim, Tae-Jin (Department of Integrated Biological Science, Pusan National University)
Publication Information
Korean Chemical Engineering Research / v.59, no.3, 2021 , pp. 366-372 More about this Journal
Abstract
Cadherin-Catenin complex is thought to play an essential role in the transmission of force at adherens junction. Due to the lack of proper tools to visualize and detect mechanical force signals, the underlying mechanism by which the cadherin-catenin complex regulates force transmission at intercellular junctions remains elusive. In this study, we visualize cadherin-mediated force transmission using an engineered α-Catenin sensor based on fluorescence resonance energy transfer. Our results reveal that α-catenin is a key force transducer in cadherin-mediated mechanotransduction at cell-cell junctions. Thus, our finding will provide important insights for studying the effects of chemical and physical signals on cell-cell communication and the relationship between physiological and pathological phenomena.
Keywords
${\alpha}$-catenin; Fluorescence resonance energy transfer (FRET); Live cell imaging; Traction force; Shear stress;
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