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http://dx.doi.org/10.9713/kcer.2021.59.1.112

Synthesis of Self-Assembled Peptide Nanoparticles Based on Dityrosine Covalent Bonds  

Hur, Yun-Mi (Biomedical Convergence Science and Technology, Kyungpook National University)
Min, Kyoung-Ik (Biomedical Convergence Science and Technology, Kyungpook National University)
Publication Information
Korean Chemical Engineering Research / v.59, no.1, 2021 , pp. 112-117 More about this Journal
Abstract
In this study, a method of self-assembly of peptides based on irreversible covalent bonds was studied by mimicking a biological covalent bond, dityrosine bond. A tyrosine-rich short peptide monomer having the sequence of Tyr-Tyr-Leu-Tyr-Tyr (YYLYY) was selected to achieve a high-density of dityrosine bond. The peptide nanoparticles covalently self-assembled with dityrosine bonds were synthesized by one-step photo-crosslinking of a peptide using a ruthenium catalyst under visible light. The effect of the concentration of each component for the size of the peptide nanoparticle was studied using dynamic light scattering, UV-Vis spectroscopy, and transmission electron microscopy. As a result, the synthesis conditions for size of the peptide nanoparticles ranging from 130 nm to 350 nm were optimized.
Keywords
Peptide; Assembly; Tyrosine; Dityrosine; Photo crosslink;
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