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http://dx.doi.org/10.9713/kcer.2018.56.3.376

Recovery of Astaxanthin from microalgae Using Simple and Energy-efficient Method  

Kim, Sun Young (Department of Advanced Materials & Chemical Engineering, Hannam University)
Oh, You-Kwan (Department of Chemical & Biomolecular Engineering, Pusan National University)
Ha, Sung Ho (Department of Advanced Materials & Chemical Engineering, Hannam University)
Publication Information
Korean Chemical Engineering Research / v.56, no.3, 2018 , pp. 376-380 More about this Journal
Abstract
The astaxanthin recovery efficiencies were compared in acetonitrile, acetone, methanol, dichloromethane : methanol (1:3, v/v) and ethylacetate : ethanol (1:1, v/v) as a extraction solvent after the grinding of the H. pluvialis cells. The astaxanthin extraction yield in acetone was 1.13~1.29 times higher than other extraction solvents. It was also found that 96.7% of astaxanthin accumulated in H. pluvialis could be recovered by a single extraction. Since astaxanthin exists mainly as astaxanthin esters in H. pluvialis, a gradient reversed-phase HPLC analysis was carried out for the separation of astaxanthin esters from the extracts of H. pluvialis. Among the astaxanthin inside the H. pluvialis cell, free astaxanthin was 45.9% and astaxanthin esters were the rest.
Keywords
Astaxanthin; Haematococcus pluvialis; Solvent extraction; Efficiency;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Kim, Z. H., Kim, S. H., Lee, H. S. and Lee, C. G., "Enhanced Production of Astaxanthin by Flashing Light Using Haematococcus pluvialis," Enzyme Microb. Technol., 39, 414-419(2006).   DOI
2 Hong, S. P., Kim, M. H. and Hwang, J. K., "Biological Functions and Production Technology of Cartenoids," J. Korean Soc. Food Sci. Nutr., 27, 1297-1306(1998).
3 Guerin, M., Huntley, M. E. and Olaizola, M., "Haematococcus Astaxanthin; Applications for Human Health and Nutrition," Trends Biotechnol., 21(5), 210-216(2003).   DOI
4 Mann, V., Harker, M., Pecker, I. and Hirschberg, J., "Metabolic Engineering of Astaxanthin Production in Tobacco Flowers," Nature Biotechnol., 18, 888-892(2000).   DOI
5 Han, D, Li, Y. and Hu, Q., "Astaxanthin in Microalgae: Pathways, Functions and Biotechnological Implications," Algae, 28, 131-147(2013).   DOI
6 Chumpolkulwong, N., Kakizono, T., Ishii, H., and Nishio, N., "Increased Astaxanthin Production by Phaffia rhodozyma Mutants Isolated as Resistant to Diphenylamine," J. Ferment. Bioeng., 83, 429-434(1997).   DOI
7 Chumpolkulwong, N., Kakizono, T., Ishii, H., and Nishio, N., "Enzymatic Conversion of Beta-carotene to Astaxanthin by Cellextracts of a Green Alga Haematococcus pluvialis," Biotechnol. Lett., 19, 443-446(1997).   DOI
8 Lim. G. B. Lee, S. Y., Lee, E. K., Haam, S. J. and Kim, W. S., "Separation of Astaxanthin from Red Yeast Phaffia rhodozyma by Supercritical Carbon Dioxide Extraction," J. Biochem. Eng., 11, 181-187(2002).   DOI
9 Lorenz, R. T. and Cysewski, G. R., "Commercial Potential for Haematococcus microalgae as a Natural Source of Astaxanthin," Trends Biotechnol., 18, 160-167(2000).   DOI
10 Schroeder, W. A. and Johnson, E. A., "Antioxidant Role of Carotenoids in Phaffia Rhodozyma," J. Gen. Microbiol., 39, 907-912 (1993).
11 Kim, S., Cho, E., Yoo, J., In, M. -J. and Chae, H. J., "Extraction and Analysis of Astaxanthin from Haematococcus," J. Korean Soc. Food Sci. Nutr., 37, 1363-1368(2008).   DOI
12 Hagen, C., Siegmund, S. and Braune, W., "Ultrastructural and Chemical Changes in the Cell Wall of Haematococcus pluvialis (Volvocales, Chlorophyta) during Aplanospore Formation," Eur. J. Phycol., 37, 217-226(2002).   DOI
13 Margalith, P. Z., "Production of Ketocarotenoids by Microalgae," Appl. Microbiol. Biotechnol., 51, 431-438(1999).   DOI
14 Boussiba, S., "Carotenogenesis in the Green Alga Haematococcus pluvialis: Cellular Physiology and Stress Response," Physiol. Plant, 108, 111-117(2000).   DOI
15 Fabregas, J., Dominguez, A., Maseda, A. and Otero, A., "Interactions Between Irradiance and Nutrient Availability During Astaxanthin Accumulation and Degradation in Haematococcus pluvialis," Appl. Microbiol. Biotechnol., 61, 545-551(2003).   DOI
16 Sarada, R., Tripathi, U. and Ravishankar, G. A., "Influence of Stress on Astaxanthin Production in Haematococcus pluvialis Grown under Different Culture Conditions," Process Biochem., 37, 623-627(2002).   DOI
17 Kim, D. Y., Vijayan, D., Praveenkumar, R., Han, J. -I., Lee, K., Park, J. Y., Chang, W.S., Lee, J. -S. and Oh, Y. -K., "Cell-wall Disruption and Lipid/astaxanthin Extraction from Microalgae: Chlorella and Haematococcus," Bioresour. Technol., 199, 300-310(2016).   DOI
18 Wellburn, A. R., "The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution," J. Plant Physiol., 144, 307-313(1994).   DOI
19 Yuan, J. P. and Chen, F., "Chromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialis," J. Agric. Food Chem., 46, 3371-3375(1998).   DOI
20 Jo, J., Shin, S., Jung, H., Min, B., Kim, S. and Kim, J., "Process Development for Production of Antioxidants from Lipid Extracted Microalgae Using Ultrasonic-assisted Extraction," Korean Chem. Eng. Res., 55, 542-547(2017).
21 Kim, J. and Ha, S. H., "Hydrothermal Pretreatment of Ulva pertusa Kjellman Using Microwave Irradiation for Enhanced Enzymatic Hydrolysis," Korean Chem. Eng. Res., 53, 570-575(2015).   DOI
22 Johnson, E. A. and An, G. H., "Astaxanthin from Microbial Sources," Crit. Rev. Biotechnol., 11, 297-326(1991).   DOI
23 Yuan, J. P. and Chen, F., "Purification of trans-Astaxanthin from a High-yielding Astaxanthin Ester-producing Strain of the Microalga Haematococcus pluvialis," Food Chem., 68, 443-448(2000).   DOI
24 Orosa, M., Franqueira, D., Cid, A. and Abalde, J., "Analysis and Enhancement of Astaxanthin Accumulation in Haematococcus pluvialis," Bioresour. Technol., 96, 373-378(2005).   DOI