Browse > Article
http://dx.doi.org/10.9713/kcer.2017.55.4.542

Process Development for Production of Antioxidants from Lipid Extracted Microalgae Using Ultrasonic-assisted Extraction  

Jo, Jaemin (Department of Food Science, Sunmoon University)
Shin, Suelgl (Department of Food Science, Sunmoon University)
Jung, Hyunjin (Department of Food Science, Sunmoon University)
Min, Bora (Department of Food Science, Sunmoon University)
Kim, Seungki (Department of Food Science, Sunmoon University)
Kim, Jinwoo (Department of Food Science, Sunmoon University)
Publication Information
Korean Chemical Engineering Research / v.55, no.4, 2017 , pp. 542-547 More about this Journal
Abstract
Ultrasound-assisted extraction (UAE) has attracted growing interest, as it is an effective method for the rapid extraction of bioactive compounds from plants with a high extraction efficiency comparable to the conventional extraction. In this study, UAE was used for the extraction of polyphenols from lipid extracted microalgae (Tetraselmis KCTC 12236BP) and the effects of five extraction variables on the total phenolic compounds (TPC) were studied. For the optimization of extraction parameters, particle size, solid-to-liquid (L/S) ratio, ethanol concentration, extraction temperature and extraction time have been examined as independent variables. All variables exhibited the significant effects on the extraction of TPC and extraction temperature showed the most significant effect among five variables. The optimal extraction conditions were the extraction using mixed particle, S/L ratio of 10%, ethanol concentration of 60%, extraction temperature of $100^{\circ}C$ and extraction time of 30 min, which gave the 8.7 mg GAE/g DW for TPC. Compared with conventional hot-water extraction, TPC extraction under UAE was increased by up to 1.8 fold with same extraction condition. This study showed that UAE under low temperature and short extraction time was proven to be an effective extraction process for TPC production from LEA compared to conventional hot-water extraction process.
Keywords
Lipid extracted microalgae; Byproduct; Polyphenol; Ultrasound assisted extraction;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Bilek, S. E., "The Effects of Time, Temperature, Solvent:solid Ratio and Solvent Composition on Extraction of Total Phenolic Compound from Dried Olive (Olea europaea L.) Leaves," J. Food., 35, 411-416(2010).
2 Stamatopoulos, K., Chatzilazarou, A. and Katsoyannos, E., "Optimization of Multistage Extraction of Olive Leaves for Recovery of Phenolic Compounds at Moderated Temperatures and Short Extraction Times," Foods, 3, 66-81(2013).   DOI
3 Alonso, E., Bourzeix, M. and Revilla, E., "Suitabilitiy of Water-ethanol Mixtures for the Extraction of catechins and proanthocyanidins from Vitis vinifera seeds contained in a winery by-product," Seed Sci. Technol., 19, 545-552(1991).
4 Yilmaz, Y. and Toledo, R. T., "Oxygen Radical Absorbance Capacities of Grape/wine Industry Byproducts and Effect of Solvent Type on extraction of grape seed polyphenols," J. Food Compos. Anal., 19, 41-48(2006).   DOI
5 Vergara-Salinas, J. R., Perez-Jimenez, J., Torres, J. L., Agosin, E. and Perez-Correa, J. R., "Effects of Temperature and Time on Polyphenolic Content and Antioxidant Activity in the Pressurized Hot Water Extraction of Deodorized Thyme (Thymus vulgaris)," J. Agric. Food Chem., 60, 10920-10929(2012).   DOI
6 Choi, W. Y., Lee, H. Y., Lee, C. G., Song, C. H., Seo, Y. C., Kim, J. S., Kim, B. H. and Lim, H. W., "Comparison of Low Molecular Ginsenoside Contents and $CO_2$ Emission from Low Quality Fresh Ginseng by Low $CO_2$ Emission Processes," Food Eng. Prog., 16, 325-332(2012).
7 Kang, Y. H., "Phenolic Compounds and Antioxidant Activity in Cell Wall Materials from Deodeok (Codonopsis lanceolata)," Korean J. Food Sci. Technol. Res., 41, 345-349(2009).
8 Kang, C. D., Park, T. H. and Sim, S. J., "Biological $CO_2$ Fixation to Antioxidant Carotenoids by Photosynthesis Using the Green Microalga Haematococcus pluvialis," Korean Chem. Eng. Res., 44(1), 46-51(2006).
9 Shin, S. L. and Lee, C. H., "Antioxidant Activities of Ostrich Fern by Different Extraction Methods and Solvents," J. Life Sci., 21, 56-61(2011).   DOI
10 Park, Y. C. and Kim, J. S., "Pretreatment of Helianthus tuberosus Residue by Two-Stage Flow Through Process," Korean Chem. Eng. Res., 53(4), 417-424(2015).   DOI
11 National Renewable Energy Laboratory, Standard Biomass Analytical Procedures, http://www.nrel.gov/biomass/analytical_procedures.html.
12 Park, Y. K. and Cho, C. H., "Effects of Additives on the Mechanical and Thermal Properties of Epoxy-based Nanocomposites Produced Using Sonication," Korean J. Chem. Eng., 33(6), 1938-1941(2016).   DOI
13 Jo, J. M., Choi, K. H., Shin, S. G., Lee, J. H. and Kim, J. W., "Optimization of Extraction Conditions of Polyphenolic Compounds from Amaranth Leaf using Statistically-based Optimization," Korean Chem. Eng. Res., 54(3), 315-319(2016).   DOI
14 Kim, T. H. and Lee, Y. Y., "Fractionation of Corn Stover by Hot Water and Aqueous Ammonia Treatment," Bioresour. Technol., 97, 224-232(2005).
15 Kim, Y. S. and Lee, K. J., "Extraction of Genistein and Formononetin from Sophoraflavescens Aiton using Ultrasonic wave," Korean Chem. Eng. Res., 47(2), 258-261(2009).
16 Chemat, F. and Khan, M. K., "Application of Ultrasound in Food Technology," Ultrasonics Sonochem., 18, 813-835(2011).   DOI
17 Jin, L., Ha, J. H., Choi, Y. Y., Seo, Y. C. and Lee, H, Y., "Enhancement of Cosmeceutical Activities of Berberis koreana Bark by High Pressure and Ultrasonification Extraction Processes," Korean Soc.Med. Crop Sci., 19, 54-65(2011).   DOI
18 Park, J. Y., LEE, G. A., Kim, K. Y., Choi, S. A., Jung, M. J. and Oh, Y. K., "Microalgal Oil Recovery by Solvent Extraction from Nannochloropsis oceanica," Korean Chem. Eng. Res., 52(1), 88-91(2014).   DOI
19 Jo, B. H. and Cha, H. J., "Biodiesel Production Using Microalgal Marine Biomass," Korean Soc. Biotechnol, Bioeng. J., 25, 109-115(2010).