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http://dx.doi.org/10.9713/kcer.2017.55.1.27

Mass Spectrometry-Based Strategy for Effective Disulfide Bond Identification  

Jin, Jonghwa (New Drug Development Center, Osong Medical Innovation Foundation)
Min, Hophil (Doping Control Center, Korea Institute of Science and Technology (KIST))
Kwon, Oh-Seung (Doping Control Center, Korea Institute of Science and Technology (KIST))
Oh, Hyun Jeong (School of Mechanical Engineering, Korea University)
Kim, Jongwon (New Drug Development Center, Osong Medical Innovation Foundation)
Park, Chulhwan (Department of Chemical Engineering, Kwangwoon University)
Publication Information
Korean Chemical Engineering Research / v.55, no.1, 2017 , pp. 27-33 More about this Journal
Abstract
The determination of disulfide bonds is important for comprehensive understanding of the chemical structure of protein. So far, many strategies for the disulfide bond analysis have been suggested in terms of speed and sensitivity. However, most of these strategies have not considered free thiol residues in the target protein in the process of determining the disulfide bond. We suggested the strategy which was composed of four steps for the identification of disulfide bonds; the first step was the prediction of possible disulfide bonds, the second step was the determination of free cysteine residues, the third step was the analysis of disulfide bond using a high-resolution mass spectrometry, and the final step was the determination of disulfide bonds based on the comprehensive verification. In this study, we performed the characterization of disulfide bonds for the recombinant protein (HRPE1), where 1 and 5 inter- and intra-chain disulfide bonds were identified, respectively.
Keywords
Disulfide bond; Free-thiol group; Mass spectrometry; Peptide mapping;
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Times Cited By KSCI : 2  (Citation Analysis)
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