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http://dx.doi.org/10.9713/kcer.2016.54.4.487

Moment Analysis (MA) of Lysozyme in Cation Exchange High Performance Liquid Chromatography (HPLC)  

Ko, Kwan Young (Department of Chemical Engineering, Chungnam National University)
Kim, In Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.54, no.4, 2016 , pp. 487-493 More about this Journal
Abstract
The moment analysis of lysozyme was implemented using chromatograms that were obtained from weak cation exchange column in high performance liquid chromatography system. Three elution sodium phosphate buffers containing 1.0, 0.75, 0.5M sodium chloride were used. Experiments were conducted by varying flow rate, elution sodium chloride concentration, and lysozyme solute concentration. The general rate (GR) model was employed to calculate the first moment and the second moment. By plotting $L/u_0$ vs. $({\mu}_1-t_0)/(1-{\varepsilon}_e)(1-{\varepsilon}_i)$] equilibrium constants (K) were obtained from first moment analysis. Intra-particle diffusivity was obtained from theoretical plate number data. Based on the results of moment analysis, van Deemter plots were drawn in order to investigate the contributions of $H_{ax}$, $H_f$, and $H_d$ to total Height Equivalent to a Theoretical Plate (HETP, $H_{total}$). The effect of intra-particle diffusion ($H_d$) was the most dominant factor contributing to HETP while external mass transfer ($H_f$) was negligible factor.
Keywords
Moment Analysis; Iysozyme;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Otto, G., "Interpretation of Asymmetric Curves in Linear Chromatography," Anal. Chem., 43(14), 1934-1937(1971).   DOI
2 Miyabe, K., "Moment Analysis of Chromatographic Behavior in Reversed-phase Liquid Chromatography," J. Sep. Sci., 32, 757-770(2009).   DOI
3 Kim, H. M., Kim, A. R., Lee, C. S., and Kim, I. H., "Identification of Proteins in Egg White using Ion Exchange Cartridge and RP-HPLC," Korean Chem. Eng. Res., 50(4), 713-717(2012).   DOI
4 Ko, K. Y. and Kim, I. H., "The Effect of pH and Temperature on Lysozyme Separation in Ion-exchange Chromatography," Korean Chem. Eng. Res., 52(1), 98-105(2014).   DOI
5 Miyabe, K. and Suzuki, M., "Chromatography of Liquid-phase Adsorption on Octadecylsilyl-silica Gel," AIChE J., 38, 901-910(1992).   DOI
6 Suzuki, M., Adsorption engineering, Kodansha, Elsevier, Tokyo, Japan(1990).
7 Liu, Z., Roininen, J., Pilkkinen, I., Saari, P., Sainio, T. and Alopaeus, V., "A New Moment Analysis Method to Estimate the Characteristic Parameters in Chromatographic General Rate Model," Computers and Chemical Engineering, 55, 50-60(2013).   DOI
8 Choi, D. Y. and Row, K. H., "Estimation of Kinetic and Rate Parameters of Peptides by Moment Analysis," J. Ind. Eng. Chem., 10, 1052-1057(2004).
9 http://www.sanderkok.com/techniques/hplc/column_properties.html.
10 Kestin, J., Khalifa, H. E. and Correia, R. J., "Tables of the Dynamic and Kinematic Viscosity of Aqueous NaCl Solutions in the Temperature Range $20-150^{\circ}C$ and the Pressure Range 0.1-35 MPa," J. Phys. Chem. Ref. Data, 10(1), 71-87(1981).   DOI