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http://dx.doi.org/10.9713/kcer.2016.54.2.223

Simple Fabrication of Adipocyte Cell Chip Using Micropatterning  

Kim, Gi Yong (Division of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI))
Jeong, Heon-Ho (Department of Chemical Engineering, Chungnam National University)
Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University)
Roh, Changhyun (Division of Biotechnology, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI))
Publication Information
Korean Chemical Engineering Research / v.54, no.2, 2016 , pp. 223-228 More about this Journal
Abstract
In this study, we described a simple and facile method to generate uniform microwells poly(dimethyl siloxane) (PDMS) microstamps through micro-molding for efficient, rapid and reliable cell patterning of adipocyte differentiation. In contrast to the conventional methods, the microstamp technologies are low expensive, non-toxic, and using a small amount of solution. Recently, Orlistat known as tetrahydrolipstatin is a prescription drug designed to treat obesity which is used to aid in weight loss and help to reduce overweight obesity. Here, 3T3-L1 cells were treated under various concentration manners of Orlistat $0.2{\mu}M{\sim}5.0{\mu}M$. and it was confirmed maximum 26.5% inhibition activity compared to control. Thus, we elucidated this platform can be used for the real-time analyzing of cell proliferation, adipocyte differentiation for evaluation of anti-obesity agents on cell chip. Furthermore, we except that this platform technology designed here might be readily be expanded to discover a wider variety of anti-obesity agents.
Keywords
Micropatterning; Anti-obesity agents; Screening; Orlistat;
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Times Cited By KSCI : 4  (Citation Analysis)
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