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http://dx.doi.org/10.9713/kcer.2016.54.1.135

Liquid-Liquid Extraction for Recovery of Paclitaxel from Plant Cell Cultures by Adding Inorganic Salts  

Ha, Geon-Soo (Department of Chemical Engineering, Kongju National University)
Kim, Jin-Hyun (Department of Chemical Engineering, Kongju National University)
Publication Information
Korean Chemical Engineering Research / v.54, no.1, 2016 , pp. 135-139 More about this Journal
Abstract
We developed a liquid-liquid extraction method using an inorganic salt to dramatically improve the recovery efficiency of the anticancer agent paclitaxel from plant cell cultures. As a result of liquid-liquid extraction using a diverse types of inorganic salt (NaCl, KCl, $K_2HPO_4$, $NaH_2PO_4$, $NaH_2PO_4{\cdot}2H_2O$), NaCl gave the highest yield (~96%) and lowest partition coefficient (0.053) of paclitaxel. The optimal NaCl/solvent ratio, methylene chloride/MeOH ratio, and pure paclitaxel content for liquid-liquid extraction using NaCl were 1% (w/v), 26% (v/v), and 0.066% (w/v), respectively. Under the optimal conditions developed in the present method, most of the paclitaxel (~96%) was recovered from biomass by a single extraction step. In addition, this method facilitated 3-fold higher recovery efficiency of paclitaxel in a shorter extraction number than the conventional liquid-liquid extraction method.
Keywords
Paclitaxel; Recovery; Liquid-Liquid Extraction; Inorganic Salt; NaCl;
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Times Cited By KSCI : 6  (Citation Analysis)
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