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http://dx.doi.org/10.9713/kcer.2015.53.4.445

Extraction of Genistein from Sophora flavescens with Supercritical Carbon Dioxide  

Han, Chang-Nam (Department of Applied Chemical Engineering, Chonnam National University)
Kang, Choon-Hyoung (Department of Applied Chemical Engineering, Chonnam National University)
Publication Information
Korean Chemical Engineering Research / v.53, no.4, 2015 , pp. 445-449 More about this Journal
Abstract
This study was directed to finding an optimum extraction condition of genistein from the S. flavescens with supercritical carbon dioxide as a solvent. In this effort, effects of the extraction conditions including pressure, temperature and a co-solvent on the extraction efficiency were investigated. The aqueous ethanol and methanol solutions were used as co-solvents while the tested operating pressure and temperature ranges were from 200 bar to 300 bar and from 308.15 K to 323.15 K, respectively. The concentration of genistein was determined by means of HPLC equipped with a UV detector. From the results, it was observed that an increase in pressure led to the higher extraction efficiency. Further, methanol showed better performance as a co-slovent than ethanol. The DPPH radical scavenging activities were measured to compare antioxidant activities of S. flavescens extracts.
Keywords
Genistein; S. flavescens; Supercritical Extraction; Co-solvent; Radical Scavenging Activity;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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1 Lee, Y. W., "Supercritical Fluid Technology (I)," News Inf. Chem. Eng., 19(3), 325-333(2001).
2 Lee, Y. W., "Supercritical Fluid Technology (II)," News Inf. Chem. Eng., 19(4), 457-467(2001).
3 Dobbs, J. M., Wong, J. M., Lahiere, R. J. and Johnston, K. P., "Modification of Supercritical Fluid Phase Behavior Using Polar Cosolvents," Ind. Eng. Chem. Res., 26, 56-65(1987).   DOI
4 Lucien, F. P. and Foster, N. R., "Solubilities of Solid Mixtures in Supercritical Carbon Dioxide: A Review," J. Supercrit. Fluids, 17, 111-134(2000).   DOI
5 Mchugh, M. A. and Krukonis, V. J., Supercritical Fluid Extraction Principles and Practice, Butterworth-Heinemann, United States, US(1986).
6 Shin, M. K., "Extract of Sophorae Radix for the Prevention and Treatment of Osteoporosis," K. R. Patent No. 2002-0044746(2000).
7 Kelly, G. E., "Treatment or Prevention of Menopausal Symptoms and Osteoporosis," U.S. Patent No. 2002/0035074A1(2000).
8 Wang, G., Kuan, S. S., Francis, O. J. and Carman, S., "A Simplified HPLC Method for the Determination of Phtoestrogens in Soybean and Its Processed Procducts," J. Agric. Food Chem., 38, 185-190(1990).   DOI
9 Peterson, G. and Barnes, S., "Genistein Inhibition of the Growth of Human Breast Cancer Cells: Independence from Estrogen Receptors and the Multi-Drug Resistance Gene," Biochem. Biophs. Res. Comm., 179, 661-667(1991).   DOI
10 Coward, L., Barnes, N. C., Setchell, K. D. R. and Barnes, S., "Genistein, Daidzein and Their $\beta$-Glycoside Conjugates: Antitumor Isoflavone in Soybean Foods from American and Asian Diets," J. Agric. Food Chem., 41, 661-667(1961).
11 Supko, J. G. and Phillips, L. R., "High Performance Liquid Chromatographic Assay for Genistein in Biological Fluids," J. Chromatogr., 666, 157-167.
12 Levitzki, A and Gazit, A., "Tyrosine Kinase Inhibition: An Approach to Drug Development," Science, 267, 1782-1787(1995).   DOI
13 Jyong, M. E. and Hwang, I. K., "Functional Components and Antioxidative Activities of Soybean Extracts," Korean Soybean Digest, 25(1), 23-29(2008).
14 So, E. H., Kuh, J. H., Park, K. Y. and Lee, Y. H., "Vrietal Difference of Isoflavone Content and Antioxidant Activity in Soybean," Korean J. Bread, 33(1), 35-39(2001).
15 Record, I. R., Dreosti, I. E. and Mclnerney, J. K., "The Antioxidant Activity of Genistein in vitro," National Biochem., 6, 481-485(1995).
16 Blois, M. S., "Antioxidant Determination by the Use of Stable Free Radical," Nature, 181, 1199-1200(1958).   DOI
17 Yang, E. S., Hong, R. H. and Kang, S. M., "The Effects of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts," Korean J. Microbiol. Biotechnol., 35(4), 316-324(2007).
18 Oh, H. S., Kim, J. H. and Lee, M. H., "Isoflavone Contents, Antioxidative and Fibrinolytic Activities of Red Bean and Mung Bean," Korean J. Soc. Food Cookepy SCI., 19(3), 263-270(2003).
19 Barnes, S. and Blair, H. C., "Genistein for Use in Inhibiting Osteroclasts," U.S. Patent No. 5, 506, 211(1996).
20 Myung, J. E. and Hwang, I. K., "Functional Components and Antioxidative Activities of Soybean Extracts," Korean Soybean Digest, 25(1), 23-29(2008).
21 Jun, H. I., Denis, P., Wiesenborn and Kim, Y. S., "Antioxidant Activities of Various Solvent Extracts from Canola Meal," Korean J. Food Preserv, 18(1), 59-64(2011).   DOI
22 Jeong, G. T., Lee, K. M. and Park, D. H., "Study of Antimicrobial and Antioxidant activities of Rumex crispus Extract," Korean Chem. Eng. Res., 44(1), 81-86(2006).