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http://dx.doi.org/10.9713/kcer.2015.53.2.211

Cultivation of Saccharomyces cerevisiae using Defatted Rice Bran Hydrolyzed in Near-critical Water as a Culture Medium  

Lee, Hong-Shik (Clean Energy Research Center, Korea Institute of Science and Technology)
Lee, Seon-Oke (School of Chemical and Biological Engineering, Seoul National University)
Ryu, Jebin (School of Chemical and Biological Engineering, Seoul National University)
Kim, Hwayong (School of Chemical and Biological Engineering, Seoul National University)
Lee, Youn-Woo (School of Chemical and Biological Engineering, Seoul National University)
Publication Information
Korean Chemical Engineering Research / v.53, no.2, 2015 , pp. 211-215 More about this Journal
Abstract
The hydrolysis of defatted rice bran using near-critical water was performed, and the feasibility of consequent hydrolyzate as a growth medium was investigated by the cultivation of Saccharomyces cerevisiae. The near-critical water hydrolysis was carried out through a series of batch experiments, and the contents of total carbohydrates, disaccharides, and monosaccharides, total organic carbon (TOC), total nitrogen (TN), pH of products were measured. The growth rate of Saccharomyces cerevisiae was measured with optical density. The yield of total carbohydrates, TOC, and TN increased with temperature below $240^{\circ}C$, however, decreased above $240^{\circ}C$. The decrease of yields above $240^{\circ}C$ was caused by the formation of organic acids, and it agreed with the change of pH of products. The yield of glucose was a maximum at $200^{\circ}C$ and it decreased dramatically at higher temperature. The growth rate of Saccharomyces cerevisiae cultivated in the hydrolyzate was similar with that in the commercial medium under certain conditions. The growth rate was correlated with the content of glucose in hydrolyzate.
Keywords
Defatted Rice Bran; Hydrolysis; Near-critical Water; Saccharomyces cerevisiae;
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Times Cited By KSCI : 2  (Citation Analysis)
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