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http://dx.doi.org/10.9713/kcer.2014.52.3.302

Effects of Fermentation Parameters on Cellulolytic Enzyme Production under Solid Substrate Fermentation  

Kim, Jin-Woo (Department of Biosystems Engineering, McGill University)
Publication Information
Korean Chemical Engineering Research / v.52, no.3, 2014 , pp. 302-306 More about this Journal
Abstract
The present study was carried out to optimize fermentation parameters for the production of cellulolytic enzymes through solid substrate fermentation of Trichoderma reesei and Aspergillus niger grown on wheat straw. A sequential optimization based on one-factor-at-a-time method was applied to optimize fermentation parameters including temperature, pH, moisture content and particle size. The results of optimization indicated that $40^{\circ}C$, pH 7, moisture content 75% and particle size between 0.25~0.5 mm were found to be the optimum condition at 96 hr fermentation. Under the optimal condition, co-culture of T. reesei and A. niger produced cellulase activities of 10.3 IU, endoglucanase activity of 100.3 IU, ${\beta}$-glucosidase activity of 22.9 IU and xylanase activity of 2261.7 IU/g dry material were obtained. Cellulolytic enzyme production with optimization showed about 72.6, 48.8, 55.2 and 51.9% increase compared to those obtained from control experiment, respectively.
Keywords
Solid State Fermentation; Co-culture; Cellullolytic Enzyme; Optimization; Biorefinery;
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Times Cited By KSCI : 4  (Citation Analysis)
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