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http://dx.doi.org/10.9713/kcer.2012.50.5.874

Effect of Buffers on Citric Acid Production by Aspergillus niger NRRL 567 in Solid Substrate Fermentation  

Kim, Jin-Woo (Department of Biosystems Engineering, McGill University)
Publication Information
Korean Chemical Engineering Research / v.50, no.5, 2012 , pp. 874-878 More about this Journal
Abstract
In the submerged fermentation of fungi, it was known pH had significant effect on the citric acid production. Various growth conditions were applied with different buffer on citric acid production by Aspergillus niger NRRL 567 grown on peat moss to find the optimum pH and most effective buffer solution. The initial pHs of different buffer solutions significantly influenced on the citric acid production and A. niger NRRL 567 produced citric acid more efficiently at high pHs. A phosphate buffer and a carbonate buffer with pH 8.6 and pH 10.0 were identified as suitable buffer solutions for citric acid production. The maximal citric acid production of 564.3 g/kg solid substrate was achieved employing carbonate buffer at pH 10.0.
Keywords
Citric Acid; Aspergillus niger; Buffer Solution; Solid Substrate Fermentation; Optimization; Peat Moss;
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1 Rodrigues, C., Vandenberghe, L. P., Teodoro, J., Pandey, A. and Soccol, C. R., "Improvement on Citric Acid Production in Solidstate Fermentation by Aspergillus niger LPB BC Mutant Using Citric Pulp," Appl. Biochem. Biotechnol., 158, 72(2009).   DOI   ScienceOn
2 Baker, S. E., Aspergillus niger Genomics: Past, Present and into the Future," Medical Mycology, 44, S17(2006).   DOI   ScienceOn
3 Yuan, X. L., Van der Kaaij, R. M., Van den Hondel, C. A., Punt, P. J., Van der Maarel, M. J., Dijkhuizen, L. and Ram, A. F., "Aspergillus niger Genome-Wide Analysis Reveals a Large Number of Novel Alpha-Glucan Acting Enzymes with Unexpected Expression Profiles," Mol Genet Genomics, 279, 545 (2008).   DOI   ScienceOn
4 Adham, Z., "Attempts at Improving Citric Acid Fermentation by Aspergillus niger in Beet-Molasses Medium," Biores. Technol., 84, 97(2002).   DOI   ScienceOn
5 Lesniak, W., Pietkiewicz, J. and Podgorski, W., "Citric Acid Fermentation from 241 Starch and Dextrose Syrups by a Trace Metal Resistant Mutant of Aspergillus niger,"Biotechnol. Lett., 24, 1065(2002).   DOI   ScienceOn
6 Nagel, F., Oostra, J., Tramper, J. and Rinzema, A., "Improved Model System for Solid-Substrate Fermentation: Effect of pH, Nutrients and Buffer on Fungal Growth Rate," Process Biochem., 35, 69(1999).   DOI   ScienceOn
7 Jianlong, W. and Ping, L., "Phytate as a Stimulator of Citric Acid Production by Aspergillus niger," Process Biochem., 33, 313 (1998).   DOI   ScienceOn
8 Abou-Zeid, A. and Ashy, M., "Production of Citric Acid: a Review," Agr. Wastes, 9, 51(1984).   DOI   ScienceOn
9 Manohar, B. and Divakar, S., "Application of Central Composite Rotatable Design to Lipase Catalysed Synthesis of m-cresyl Acetate," W. J. Microbiol. Biotechnol., 18, 745(2002).   DOI   ScienceOn
10 Barrington, S., Choiniere, D., Trigui, M. and Knight, W., "Effect of Carbon Source on Compost Nitrogen and Carbon Losses," Biores. Technol., 83, 189(2002).   DOI   ScienceOn
11 Marier, J. R. and Boulet, M., "Direct Determination of cItric Acid In Milk with Improved Pyridine-Acetic Anhydride Method," Dairy Sci., 41, 1683(1958).   DOI
12 Miller, G. L., "Use of Dinitrosalycilic Acid Reagent for Determination of Reducing Sugars," Ana. Chem., 31, 426(1959).   DOI
13 Kumar, D., Jain, V. K., Shanker, G. and Srivastava, A., "Utilisation of Fruits Waste for Citric Acid Production by Solid State Fermentation," Process Biochem., 38, 1731(2003).   DOI   ScienceOn
14 Rezaei, P. S., Darzi, G. N. and Shafaghat, H., "Optimization of the Fermentation Condition and Partial Characterization for Acido-Thermophilic $\alpha$-Amylase from Aspergillus Niger NCIM 548," Korean J. Chem. Eng., 27, 3(2010).
15 Hang, Y. D., Luh, B. S. and Woodams, E. E., "Microbial Production of Citric Acid by Solid State Fermentation of Kiwifruit Peel," J. Food Sci., 52, 226(1987).   DOI
16 Hang, Y. D. and Woodams, E. E., "Production of Citric Acid from Corncobs by Aspergillus niger ," Bioresour. Technol., 65, 251(1998).   DOI   ScienceOn
17 Roukas, T., "Citric and Gluconic Acid Production from Fig by Aspergillus niger Using Solid-State Fermentation," J. Ind. Microbio. Biotechnol., 25, 298(2000).   DOI
18 Uyar, F. and Baysal, Z., "Production and Optimization of Process Parameters for Alkaline Protease Production by a Newly Isolated Bacillus sp. Under Solid State Fermentation," Process Biochem., 39, 1893 (2003).
19 Fawole, O. B. and Odunfa, S. A., "Some Factors Affecting Production of Pectin Enzymes by Aspergillus niger," Int. Biodeterioration, 51, 223(2003).
20 Kamini, N. R., Mala, J. G. S. and Puvanakrishnan, R., "Lipase Production from Aspergillus niger, by Solid-state Fermentation Using Gingelly Oil Cake," Process Biochem., 33, 505(1998).   DOI   ScienceOn
21 Watanabe, T., Suzuki, A., Nakagawa, H., Kirimura, K. and Usami S., "Citric Acid Production from Cellulose Hydrolysate by 2-Deoxyglucose Resistant Mutant Strain of Aspergillus niger," Bioresour. Technol., 66, 271(1998).   DOI   ScienceOn
22 Bellon-Maurel, V., Orliac, O. and Christen, P., "Sensor and Measurements in Solid State Fermentation: a Review," Process Biochem., 38, 881(2003).   DOI   ScienceOn
23 Fujio, Y., Ogata, M. and Ueda, S., "Ethanol Fermentation of Raw Cassava Starch with Rhizopus Koji in a Gas Circulation Type Fermentor," Biotechnol. Bioeng., 27, 1270(1985).   DOI   ScienceOn
24 Tengerdy, R. P., "Solid State Fermentation," Trends Biotechnol., 3, 96(1985).   DOI   ScienceOn
25 Omori, T., Takeshima, N. and Shimoda, M., "Formation of Acid-Labile $\alpha$-Amylase During Barley-kogi Production," J. Ferm. Bioeng., 78, 27(1994).   DOI   ScienceOn
26 Szakacs, G. and Tengerdy, R. P., "Production of Cellulose and Xylanase with Selected Filamentous Fungi by Solid Substrate Fermentation," ACS sym. series. Washington D.C., 175(1996).
27 Gutierrez-Correa, M., Portal, L., Moreno, P. and Tengerdy, R. P., "Mixed Culture Solid Substrate Fermentation of Trichoderma reesei with Aspergillus niger on Sugar Cane Bagasse," Bioresour. Technol., 68, 173(1999).   DOI   ScienceOn
28 Ellaiah, P., Srinivasulu, B. and Adinarayana, K., "Optimization Studies on Neomycin Production by Mutant Strain Streptomyces Marinesis in Solid State Fermentation," Process Biochem., 39, 529(2004).   DOI   ScienceOn
29 Goes, A. P. and Sheppard, J. D., "Effect of Surfactant on $\alpha$-Amylase Production in a Solid Substrate Fermentation Process," J. Chem. Technol. Biotechnol., 73, 709(1999).
30 Barrington, S., Kim, J. S., Wang, L. and Kim, J. W., "Optimization of Citric Acid Production by A. niger Grown in a Column Bioreactor," Korean J. Chem. Eng., 29, 2(2009).
31 Robinson, T., Singh, D. and Nigam, P., "Solid-state Fermentation; a Promising Microbial Technology for Secondary Metabolite Production," Appl. Microbiol. Biotechnol., 55, 284(2001).   DOI   ScienceOn