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http://dx.doi.org/10.9713/kcer.2011.49.5.588

Improvement of Porcine Epidemic Diarrhea Disease Vaccine Productivity by Ammonium Ion Removal in a Carberry Type Bioreactor  

Lee, Chang-Jin (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
Jeong, Yeon-Ho (Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University)
Publication Information
Korean Chemical Engineering Research / v.49, no.5, 2011 , pp. 588-593 More about this Journal
Abstract
The porcine epidemic diarrhea virus(PEDV) production yield in spinner flask cultures using Vero cells immobilized on microcarriers was improved by the selective adsorption of ammonium ions in a Carberry type bioreactor which was equipped with Phillipsite-Gismondine synthetic zeolite. Though the apparent cell growth seemed to be lower than that of control due to the aggregation of microcarriers between impeller shaft and the adsorbent, zeolite was found to not to be toxic to Vero cell, considering estimated glucose and lactate changes. Zeolite was observed to remove ammonium ions effectively in both steps of cell growth and virus production. In virus production, the virus titer with zeolite was two times higher than that without zeolite. Consequently, zeolite was found to be an ideal adsorbent for higher production of virus vaccine with the effective removal of ammonium ions.
Keywords
Vero Cell; Porcine Epidemic Diarrhea Virus; Vaccine; Ammonium Ion; Zeolite; Carberry Type Bioreactor;
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