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Characteristics of Wheat Germ Oil during Enzymatic Ethanolysis in Supercritical Carbon Dioxide  

Back, Sung-Sin (Faculty of Food Science and Biotechnology, Pukyong National University)
Kwon, Kyung-Tae (Faculty of Food Science and Biotechnology, Pukyong National University)
Jung, Go-Woon (Faculty of Food Science and Biotechnology, Pukyong National University)
Ahn, Hyaung-Min (Faculty of Food Science and Biotechnology, Pukyong National University)
Sim, Jeong-Eun (Faculty of Food Science and Biotechnology, Pukyong National University)
Kang, Hee-Moon (Faculty of Food Science and Biotechnology, Pukyong National University)
Chun, Byung-Soo (Faculty of Food Science and Biotechnology, Pukyong National University)
Publication Information
Korean Chemical Engineering Research / v.47, no.5, 2009 , pp. 546-552 More about this Journal
Abstract
Enzymatic ethanolysis of wheat germ oil with immobilized lipase was investigated for enhancing the function of wheat germ oil. Ethanolysis reactions were carried out in two different systems; non-pressurized and pressurized system. In non-pressurized system, the enzymatic ethanolysis was carried out in an erlenmeyer flask(25 ml) containing a mixture of wheat germ oil and 99.90% ethanol using 1~5 wt% immobilized lipase as Lipozyme TL-IM and Lipozyme RM-IM and the reaction mixtures were incubated at $40{\sim}70^{\circ}C$ with 120 rpm shaking. In pressurized system, the enzymatic ethanolysis was carried out at various condition; immobilized lipase concentration(2 wt%), reaction time(24 h), reaction temperature($40{\sim}60^{\circ}C$) and reaction pressure(75, 100, 150, 200 bars). The samples obtained from each fraction were analyzed by HPLC for analysing contents of monoglyceride, diglyceride, and triglyceride. The conversion of wheat germ oil relied on the reaction temperature and the concentration of immobilized lipase. The optimum condition of enzymatic ethanolysis in non-pressurized and pressurized systems was at $50^{\circ}C$ and 100 bar.
Keywords
Wheat Germ Oil; Supercritical Carbon Dioxide; Immobilized Lipase; Enzymatic Ethanolysis; Monoglyceride; Diglyceride; Triglyceride; HPLC;
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