Browse > Article

Effect of Temperature and Eluent Composition on the Separation of Ketoprofen and Ibuprofen Racemates in Kromasil HPLC Column  

Park, Moon-Bae (Department of Chemical Engineering, Chungnam National University)
Kim, In Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.47, no.1, 2009 , pp. 54-58 More about this Journal
Abstract
Ketoprofen and ibuprofen are non-steroid anti-inflammatory drug(NSAID) that have analgesic and antipyretic properties. (S)-ketoprofen and (S)-ibuprofen have pharmacological activity, while (R)-ketoprofen and (R)-ibuprofen are either inactive or have side effect. The chiral separation of racemic ketoprofen and ibuprofen enantiomers was carried out by using a Kromasil HPLC column. Some chromatographic parameters (selectivity, resolution, number of theoretical plates and ${\Delta}H$) are calculated under different mobile phase compositions of hexane/t-BME/acetic acid and temperatures. The selectivity, resolution and number of theoretical plates were observed high at $25^{\circ}C$ and the composition of hexane/t-BME/acetic acid (80/20/0.1).
Keywords
Ketoprofen; Ibuprofen; Kromasil Column;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Boisvert, J., Caille, G., McGilveray, I. J. and Qureshi, S. A., "Quantification of Ketoprofen Enantiomers in Human Plasma Based on Solid Phase Extraction and Enantioselective Column Chromatography," J. Chromatogr. B, 690, 189(1997)   DOI   ScienceOn
2 Song, T. H., Suh, S. S., Choi, M. H., Kim, Y. D., Lee, J. K. and Park, D. K., 'Optical Resolution of Racemic Ibuprofen by using Chiral Stationary Phase,' HWAHAK KONGHAK, 38(3), 373-379 (1999)
3 Park, T. H., Yoon, T. H. and Kim, I. H., "Simplistic Determination of Operation Parameters for Simulated Moving Bed (SMB) Chromatography for the Separation of Ketoprofen Enantiomer," Biotechnology and Bioprocess Engineering., 10(4), 341-345(2005)   DOI   ScienceOn
4 Meyring, M., Chankvetadze, B. and Blaschke, G., "Simultaneous Separation and Enantioseparation of Thalidomide and its Hydroxylated Metabolites using High-Performance Liquid Chromatography in Common-size Columns, Capillary Liquid Chromatography and Nonaqueose Capillary Electrochromatography," J. Chromatogr. A, 876, 157(2000)   DOI   PUBMED   ScienceOn
5 Yan, H. Y. and Row, K. H., "Optimum Operation Condition for Chiral Speration of Tryptophan Enantiomers Using Ligand Exchange Liquid Cromatogaphy," Biotech. Bioproc. Eng., 12, 235(2007)   DOI   ScienceOn
6 Hyun, M. H., Separation of Optical Isomers by LC, Minumsa, Seoul(1996)
7 Yoon, T. H. and Kim, I. H., "Chiral Separation of Racemate by using Chirex 3005 and Kromasil CHI-II Chral Column," Korean J. Chem. Eng., 21(2), 521-526(2004)   DOI   ScienceOn
8 Thomas, E. B. and Raymond, P. W. S., "Chiral Chromatography," (1998)
9 Pietta, P., Manera, E. and Ceva, P., "Purity Assay of Ketoprofen by High Performance Liquid Chromatography," J. Chromatogr., 387, 525(1987)   DOI   ScienceOn
10 Kim, S. Y., Lee, J. K., Suh, S. S., Choi, M. H., Park, D. K. and Park, T. J., 'Optical Separation of Racemic Ketoprofen by Chiral High-performance Liquid Cromatography,' HWAHAK KONGHAK, 39(6), 703(2001)