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Optimization of Bovine Testicular PH-20 hyaluronidase Production in Pichia pastoris  

Shin, Hwa Shook (Department of Biological Engineering, Inha University)
Kim, Eunki (Department of Biological Engineering, Inha University)
Publication Information
Korean Chemical Engineering Research / v.46, no.4, 2008 , pp. 764-768 More about this Journal
Abstract
Bovine testicular hyaluronidase PH-20 was cloned into pPIC9 vector and expressed in Pichia pastoris. Recombinant PH-20 was 75 kDa MW and 7460 units/L activity. Extracellular hyaluronidase activity was two times higher than that of intracellular activity. Non-buffered medium and $30^{\circ}C$ cultivation was favorable for PH-20 production. 1M sorbitol as an osmotic pressure and 0.3% methanol inducer increased cell growth and enzyme activity. 0.4 M arginine augmentation decreased the proteolytic degradation of recombinant hyaluronidase.
Keywords
Bovine Testicular; Hyaluronidase; PH-20; Pichia pastoris;
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