Browse > Article

Biomineralization Strategy of Biocomposites on Regenerated Shell: Chitin Synthesis and Regenerated Shell Formtation by Deformed Oyster Shell  

Lee, Seungwoo (Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Park, Seungbin (Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
Yeong, Donghee (Department of Chemical and Biomolecular Engineering, Sogang University)
Choi, Cheongsong (Department of Chemical and Biomolecular Engineering, Sogang University)
Publication Information
Korean Chemical Engineering Research / v.46, no.3, 2008 , pp. 529-534 More about this Journal
Abstract
The normal shell and the regenerated oyster shell, Crassostrea gigas, are separated according to the characteristics of inner shell morphology. To study characteristics of chitin obtained from the regenerated shell, chitin prepared by acid and alkali process is analyzed by FT-IR (Fourier transform infrared spectrometer) and XRD (X-ray Diffractometer). The content of insoluble protein in the normal shell was more than doubled as compared with that in the regenerated shell. A comparison of secondary structure of the normal shell and the regenerated shell revealed that the content of random of the regenerated shell was above 47%, indicating an amount in the structural unordered state. Through amino acid composition analysis and secondary protein structure of soluble protein isolated from the normal shell and the regenerated shell, it was found that there are differences in biomineralization strategy of the regenerated shell as compared to the normal shell. The relatively low hardness of the regenerated shell is caused by the change of amino acid composition and ordered secondary protein structure as compared to hardness of the normal shell.
Keywords
Crassostrea gigas; Regenerated Shell; ${\beta}-chitin$; Biocomposites; Amino Acid Composition; Secondary Protein Structure;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hirano, S., Nagamura, K., Zhang, M., Kim, S. K., Chung, B. G., Yoshikawa, M. and Midorikawa, T., "Chitosan Staple Fibers and Their Chemical Modification with Some Aldehydes," Carabohyrate Polymer, 38, 293-298(1999)   DOI   ScienceOn
2 Jeuniaux, C., in S. P. Colowick and N. O. Kaplan (Ed.) Methods in enzymology, Acad. Press, New York, 644-650(1966)
3 Falini, G., Weiner, S. and Addadi, L., "Chitin-Silk Fibroin Interactions: Relevance to Calcium Carbonate Formation in Invertebrates," Calcified Tissue International, 72, 548-554(2003)   DOI
4 Blake, J. A. and Evans, J. W., "Polydora and Related Genera as Bores in Mollusk Shells and Other Calcareous Substrates," The Veliger, 15, 235-249(1973)
5 Mann, S., Biomineralization principles and concepts in bioinorganic materials chemistry. 1st ed., Oxford University Press, New York(2001)
6 Lowenstam, H. A. and Weiner, S., On biomineralization. 1st ed., Oxford University Press, New York(1989)
7 Davis, J. G., Oberholtzer, J. C., Burns, F. R. and Greene, M. L., "Molecular Cloning and Characterization of an Inner Ear-Specific Structural Protein," Science, 267, 1031-1034(1995)   DOI   ScienceOn
8 Lee, S. W., Hong, S. M. and Choi, C. S., "Characteristics of Calcification Processes in Embryos and Larvae of the Pacific Oyster, Crassostrea gigas," Bull. Mar. Sci. 78, 309-316(2006)
9 Addadi, L. and Weiner, S., "Interactions Between Acidic Proteins and Crystals: Stereochemical Requirements in Biomineralization," Proc. Natl. Acad. Sci. U.S.A., 82, 4110-4114(1985)
10 Cho, S. M, "Effect of Polycyclic Aromatic Hydrocarbons (PAHs) on the Pacific Oyster, Crassostrea gigas," Ph.D. Dissertation- Gyeongsang National University, Korea(2006)
11 Badariotti, F., Thuau, R., Lelong, C., Dubos, M. P. and Favrel, P., "Characterization of An Atypical Family 18 Chitinase from the Oyster Crassostrea gigas: Evidence for a Role in Early Development and Immunity," Dev. & Comp. Immunol., 31, 559-570(2007)   DOI   ScienceOn
12 Park, J. K., "Industrial Application of Chitinase and Chtosanase," J. Chitin & Chitosan, 7, 1-7(2002)
13 Fereidoon, S., Janak, K. and Jeon, Y. J., "Food Applications of Chitin and Chitosans," Trends in Food Sci. & Tech., 10, 37-51 (1999)   DOI   ScienceOn
14 Kwon, J. K., Kong, B. G. and Jang, M. K., "Thermdynamic Characterzation of $\alpha-,\;\beta-,\;and\;\gamma$-Chitin," J. Chitin & Chitosan, 7, 154-160(2002)
15 Lee, S. W. and Choi, C. S., "The Correlation Between Organic Matrices and Biominerals (myostracal prism and folia) of the Adult Oyster Shell," Micron, 38, 58-62(2007)   DOI   ScienceOn
16 Shagemasa, Y. and Minami, S., "Applications of Chitn and Chtosan for Biomaterials," Biotech. Gen. Engi. Rev., 13, 1353-1359(1995)
17 Medakovic, D., Popovic, S., Crzeta, B., Plazonic, M. and Hrs- Brenko, M., "X-ray Diffraction Study of Calcification Processes in Embryos and Larvae of the Brooding Oyster Ostrea edulis," Mar. Biol., 129, 615-623(1997)   DOI
18 Bowen, C. E. and Tang, H., "Conchiolin-Protein in Aragonite Shells of Mollusks," Comp. Biochem. Physiol., 115A (4), 269-275(1996)
19 Willis, J., "Cutcular Proteins in Insects and Crustaceans," Am. Zool., 39 600-609(1999)   DOI
20 Jeuniaux, C., in; M. Florkin and E.H. Stotz (Ed.), Comprehensive Biochemistry (Chitinious Structure), Elsevier, New York, 595-631(1971)
21 Kifune, K., Medical application of chitin/chitosan, Gibodang Press, Japan(1994)
22 Mount, A. S., Wheeler, A. P., Paradkar, R. P. and Snider, D., "Hemocyte-Mediated Shell Mineralization in the Eastern Oyster," Science, 304, 297-300(2004)   DOI   ScienceOn
23 Weiner, S. and Traub, W., "X-ray Diffraction Study of the Insoluble Organic Matrix of Mollusk Shell," FEBS Lett., 111, 311-318 (1980)   DOI   ScienceOn
24 Kifune, K., in; C. J. Brine, P. A. Sandford and J. P. Zikakis (Ed.), "Advances in Chitin and Chitosan," Elsevier Science, London, 9- 15(1992)
25 Kranz, B., Burck, J., Franzreb, M., Koster, R. and Ulrich, A. S., "Circular Dichroism Analysis of Peniciline G Acylase Covalently Immobilized on Silica Nanoparticles," J. Collid. Interface Sci. 316, 413-419(2007)   DOI   ScienceOn