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Fabrication of Non Viral Vector for Drug and Gene Delivery using Particle Replication In Non-Wetting Templates (PRINT) Technique  

Park, Ji-Young (School of Pharmacy, University of North Carolina at Chapel Hill)
Gratton, Stephanie (Department of Chemistry, University of North Carolina at Chapel Hill)
Benjamin, Maynor (Department of Chemistry, University of North Carolina at Chapel Hill)
Lim, Jomg Sung (Department of Chemical and Biomolecular Engineering, Sogang University)
Desimone, Joseph (Department of Chemistry, University of North Carolina at Chapel Hill)
Publication Information
Korean Chemical Engineering Research / v.45, no.5, 2007 , pp. 493-499 More about this Journal
Abstract
Polymeric hydrogel particles were fabricated to demonstrate the scale-up possibilities with the Particle Replication In Non-wetting Templates (PRINT) process. A permanently etched, specifically designed master was made on a silicon wafer using conventional photolithography, then reactive ion etching. The master and substrate were used repeatedly to make a large number of identical elastomeric perfluoropolyethers (PFPE) replica molds. The PFPE replica molds were used to fabricate and harvest individual, monodisperse micron-sized particles using the PRINT process. A water-soluble polymer adhesive was used as a sacrificial layer for harvesting particles. Particles were composed of biodegradable poly (ethylene glycol) diacrylate (PEG-diA), and aminoethylacrylate (AEM) and 2-acryloxyethyltrimethyl ammonium chloride (AETMAC) were added to them for improving the uptake of the cells. This study suggested PRINT used to produce the uniformed and shape specific biodegradable polymer is the effective technique for the non viral vector for the drug and the gene delivery.
Keywords
Polymeric Hydrogel Particles; Particle Replication In Non-wetting Templates (PRINT) Process; Photolithography; Perfluoropolyethers (PFPE);
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1 Niidome, T. and Huang, L., 'Gene Therapy Progress and Prospects: Nonviral vectors,' Gene Therapy, 9(24), 1647-1652(2002)   DOI   ScienceOn
2 Kay, M. A., Glorioso, J. C. and Naldini, L., 'Viral Vectors for Gene Therapy: the Art of Turning Infectious Agents Into Vehicles of Therapeutics,' Nature Medicine, 7, 33-40(2001)   DOI   ScienceOn
3 Verma, I. M. and Somia, S. N., 'Promises, Problems and Prospects,' Nature, 389(6648), 239-242(1997)   DOI   ScienceOn
4 Xia, Y. and Whitesides, G. M., 'Soft Lithography,' Angew. Chem. Int. Ed, 37(5), 550-575(1998)   DOI   ScienceOn
5 Rolland, J. P., Maynor, B. W., Euliss, L. E., Exner, A. E., Denison, G. M. and Desimone, J. M., 'Direct Fabrication and Harvesting of Monodisperse, Shape-Specific Nanobiomaterials,' J. Amer. Chem. Soc., 127(28), 10096-10100(2005)   DOI   ScienceOn
6 Rolland, J. P., Van Dam, R. M., Schorzman, D. A., Quake, S. R. and Desimone, J. M., 'Solvent-Resistant Photocurable 'Liquid Teflon' for Microfluidic Device Fabrication,' J. Amer. Chem. Soc., 126(8), 2322-2323(2004)   DOI   ScienceOn
7 Larken, E. E., Christopher, M. W., Maynor, B. W., Rolland, J. P., Denison, M. G., Gratton, S. E., Park, J. Y., Ashish, P. A., Elizabeth, E. L., Juliano, R. L., Hahn, K. M. and Desimone, J. M., 'Monodisperse Nanocarriers: Novel Fabrication of Polymeric Nanoparticles for Bio-nanotechnology,' SPIE-The International Society for Optical Engineering, Advances in Resist Technology and Processing, 32-34(2006)
8 Park, J. Y., Maynor, B. W., Pandya, A., Gratton, S. E. and Desimone, J. M., 'Fabrication and Harvest of Polymeric Micro, and Nano Particles Using Particle Replication in Non-wetting Templates (PRINT),' NSF-STC fall meeting, UNC, 18-19(2005)
9 Lu, Q. L., Gharios, G. B. and Partridge, T. A., 'Non-Viral Gene Delivery in Skeletal Muscle: a Protein Factory,' Gene Therapy, 10(2), 131-142(2003)   DOI   ScienceOn
10 Nishikawa, M. and Huang, L., 'Nonviral Vectors in the New Millennium: Delivery Barriers in Gene Transfer,' Human Gene Therapy, 12(8), 861-870(2001)   DOI   ScienceOn
11 Schmid, H. and Michel, B., 'Siloxane Polymers for High-Resolution, High-Accuracy Soft Lithography,' Macromolecules, 33(8), 3042-3049(2000)   DOI   ScienceOn
12 Herweijer, H. and Wolff, J. A., 'Progress and Prospects: Naked DNA Gene Transfer and Therapy,' Gene Therapy, 10(6), 453-458 (2003)   DOI   ScienceOn
13 Whitesides, G. M., Ostuni, E., Takayama, S., Jiang, X. and Ingber, D. E., 'Soft Lithogaphy in Biology and Biochemistry,' Annual Review of Biomedical Engineering, 3, 335-373(2001)   DOI   ScienceOn
14 Mulligan, R. C., 'The Basic Science of Gene Therapy,' Science, 260(5110), 926-932(1993)   DOI