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Influence of Ionic Liquid for Separation of D-tryptophan and N-CBZ-D-phenylalanine  

Jin, Yinzhe (Center for Advanced Bioseparation Technology, Chemical Engineering & Biotechnology, Inha University)
Zheng, Jinzhu (Center for Advanced Bioseparation Technology, Chemical Engineering & Biotechnology, Inha University)
Polyakova, Yulia (Center for Advanced Bioseparation Technology, Chemical Engineering & Biotechnology, Inha University)
Koo, Yoon Mo (Center for Advanced Bioseparation Technology, Chemical Engineering & Biotechnology, Inha University)
Row, Kyung Ho (Center for Advanced Bioseparation Technology, Chemical Engineering & Biotechnology, Inha University)
Publication Information
Korean Chemical Engineering Research / v.44, no.5, 2006 , pp. 453-459 More about this Journal
Abstract
D-tryptophan and N-CBZ-D-phenylalanine were separated using ionic liquid as additives for the mobile phase in high performance liquid chromatography (HPLC). The ionic liquid of 1-butyl-3- methylimidazolium tetrafluoroborate (${[BMIm]}^+{[BF_4]}^-$) was used. Mobile phases were 65％, 70％, and 80％ methanol in water with addition of different concentrations (0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, 10.0, 12.0, and 15.0 mmol/L) of the ionic liquid. The experiments were performed on stainless steel column, $3.9{\times}300mm$ i.d., packed with $15{\mu}m$ octadecyl-bonded silica gel at laboratory.The retention factor of D-tryptophan was not negligibly changed while that of N-CBZ-D-phenylalanine was decreased. The resolution between the two components were affected by the contents of methanol and ionic liquid in the mobile phase. With the small content of methanol and the high concentration of ionic liquid, the resolution was improved.
Keywords
D-tryptophan; N-CBZ-D-phenylalanine; Ionic Liquid; Retention Factor; Resolution;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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