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Recovery of Aglycone of Daidzein and Genistein in Soybeans  

Lee, Kwang Jin (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Jin, Long Mei (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Kim, Young Sik (Department of Chemical Engineering, Samcheok National University)
Row, Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Publication Information
Korean Chemical Engineering Research / v.43, no.5, 2005 , pp. 641-645 More about this Journal
Abstract
Using aqueous solution of 60% ethanol, the isoflavones contained in the Soybean (Chungsun, Korea), Tawon (Suwon, Korea), Jackbean (Hongsung, Korea), Soybean (Jilin, China), Black Soybean (Jilin, China), Kidneybean (Jilin, China) and Soybean (California, U.S.A.) were extracted. In this work, the pretreatment step was composed of agitation extraction, filtration, concentration, ultracentrifuge, and membrane filtration. The analysis by $C_{18}$ column was performed, and the mobile phase applied was linearly changed with A/B of 85/15-65/35 vol% for 50 min (A water/acetic acid, 99.9/0.1 vol%, B acetonitrile/acetic acid, 99.9/0.1 vol%). Among the soybeans tested, the total amounts of the four isoflavones (daidzin, genistin, daidzein, and genistein) extracted by the soybeans from Korea, China and U.S.A. were 1.37, 1.60, and 2.25 mg/g, respectively. It was also found that the total amount of aglycone of daidzein and genistein from soybean (American California) was $144{\mu}g/g$, which was the largest for the soybeans experimented.
Keywords
Recovery; Solvent Extraction; Daidzein; Genistein; Soybean;
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