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DNA Condensation and Delivery in 293 Cells Using Low Molecular Weight Chitosan/gene Nano-complex  

Pang, Shi-Won (Office of Technical Instructors, Small Business Training Institute)
Jang, Yangsoo (Yonsei Cardiovascular Research Institute, College of Medicine, Yonsei University)
Kim, Jung-Hyun (Department of Chemical Engineering, Yonsei University)
Kim, Woo-Sik (Department of Chemical Engineering, Yonsei University)
Publication Information
Korean Chemical Engineering Research / v.43, no.2, 2005 , pp. 313-317 More about this Journal
Abstract
Synthetic gene carriers such as poly-cationic polymers easily form complexes with plasmid DNA which contains negative charge. Chitosan is a polysaccharide that demonstrates much potential as a gene delivery system. The ability of depolymerized chitosan to condense DNA was determined using electrophoresis. Dynamic laser scattering and scanning electron microscopy were used to examine the size and the morphology of the chitosan/DNA complex. Parameters such as chitosan molecular weight and charge density influenced the complex size and the DNA amount condensed with chitosan. The cell viabilities in the presence of chitosan ranged between 84-108% of the control in all experiments. Gene expression efficacy using chitosan/DNA complex was enhanced in 293 cells relative to that using naked DNA, although it was lower than that using lipofecamine. Transfection efficacy using low molecular weight chitosan (Mw=8,517) was higher than those of the control and the other chitosan (MW=4,078). The low molecular weight chitosan (MW=8,517) with a high charge density (18.32 mV) fulfilled the requirements for a suitable model gene delivery system with respect to the condensing ability of DNA, complex formation, and transfection efficacy.
Keywords
Gene Delivery; Chitosan; Molecular Weight; Transfection; Nano Complex;
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