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http://dx.doi.org/10.13045/acupunct.2016033

The Effects of LR3 and SP6 Acupuncture on Liver Damage of Streptozotocin-induced Diabetic Mice  

Kim, Sung Jin (Dept. of Acupuncture & Moxibustion medicine, College of Korean Medicine, Daegu Haany University)
Lee, Yun Kyu (Dept. of Acupuncture & Moxibustion medicine, College of Korean Medicine, Daegu Haany University)
Lee, Hyun Jong (Dept. of Acupuncture & Moxibustion medicine, College of Korean Medicine, Daegu Haany University)
Kim, Jae Soo (Dept. of Acupuncture & Moxibustion medicine, College of Korean Medicine, Daegu Haany University)
Publication Information
Journal of Acupuncture Research / v.33, no.3, 2016 , pp. 29-43 More about this Journal
Abstract
Objectives : The purpose of this study was to investigate the effect of LR3 and SP6 acupuncture on liver damage of streptozotocin-induced diabetic mice. Methods : Male ICR mice were divided into four groups, consisting of the normal mice group(N), acupuncture-free diabetic mice group(Con), LR3-acupuncture diabetic mice group(LR3) and SP6-acupuncture diabetic mice group(SP6). The following measurements were taken: Body weight, food intake and water intake for 2 weeks; liver weight, and glucose levels in the serum and liver; ALT and AST in the serum; reactive oxygen species(ROS), reduced glutathione(GSH) and oxidized glutathione(GSSG) in the liver; and lastly, receptor for advanced glycation endproducts( RAGE), $N{\varepsilon}-carboxymethyl$ lysine(CML), $N{\varepsilon}-carboxyethyl$ lysine(CEL), phosphorylation of inhibitory kappa B alpha($p-I{\kappa}B{\alpha}$), nuclear factor-kappa B($NF-{\kappa}B$), activator protein-1(AP-1), cyclooxygenase-2(COX-2), inducible nitric oxide synthase(iNOS), tumor necrosis factor-alpha($TNF-{\alpha}$), ${\beta}-actin$, cytochrome c and caspase in the liver. Results : The liver weight and GSH/GSSG ratio were significantly increased in SP6 compared to Con. The glucose levels in the liver were significantly decreased in LR3 compared to Con. The generation of ROS and GSSG were significantly decreased in SP6 compared to Con. The expressions of RAGE, CML, AP-1, $TNF-{\alpha}$, cytochrome c and caspase 3 were significantly decreased in LR3 compared to Con. The expressions of $p-I{\kappa}B{\alpha}$, $NF-{\kappa}B$, AP-1, COX-2, iNOS and caspase 3 were significantly decreased in SP6 compared to Con. Conclusion : It is predicted that LR3 acupuncture is related to reduced glucose levels in the liver and expressions of AGE, and that, SP6 acupuncture is related to reduced oxidative stress-related transcription factors and inflammation-related proteins. Therefore, we suggest that LR3 and SP6 acupuncture have protective effects on the liver of streptozotocin-induced diabetic mice by preventing apoptosis.
Keywords
LR3; SP6; Acupuncture; Streptozotocin; Liver damage; Oxidative stress;
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Times Cited By KSCI : 10  (Citation Analysis)
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1 Han YK, Park YK. The comparisons of Lycii Radicis Cortex and Corni Fructus water extract effects on streptozotocin-induced diabetes in rats. Korean J Herbology. 2013 ; 28(6) : 71-7.
2 Oh TW, Kang SY, Kim KH, Song MY, Park YK. Anti-diabetic effect of medicinal plants used for lower wasting-thirst in streptozotocin-induced diabetic rats. Kor. J. Herbology. 2013 ; 28(5) : 53-60.
3 Korean Meridians&Acupoints society teaching meterial editing committee. Details of Meridians& Acupoints;A guidebook for college student the first. Wonju : Uibang. 2009 : 282,918.
4 Youn DH, Na CS. Hepatoprotective effects of electro-acupuncture at taechung(LR3) and Yangji(TE4) on experimental liver injury in rats. Korean J Acupuncture. 2006 ; 23(2) : 167-76.
5 Jung JC, Kim KH, Park YC et al. The study on the effect of acupuncture on UPDRS and heart rate variability in the patients with idiopathic parkinson's disease. The Acupunct. 2006 ; 23(3) : 143-53.
6 Lee BC, Lee HS, Kim KS et al. Effects of hap-Kok(LI-4), samUmGyo(SP-6) acupuncture on uterine motility and cyclooxygenase - 2 manifestation in rats. The Acupunct. 2000 ; (2) : 187-208.
7 Shi L, Zhang HH, Xiao Y, Hu J, Xu GY. Electroacupuncture suppresses mechanical allodynia and nuclear factor ${\kappa}$ B signaling in streptozotocin-induced diabetic rats. CNS Neurosci Ther. 2013 ; 19(2) : 83-90.   DOI
8 Jiang YL, Ning Y, Liu YY et al. Effects of preventive acupuncture on streptozotocin-induced hyperglycemiainrats. JEndocrinolInvest. 2011 ; 34(10) : 355-61.
9 Nokoff N, Rewers M. Pathogenesis of type 1 diabetes: lessons from natural history studies of high-risk individuals. Ann N Y Acad Sci. 2013 ; 1(1) : 1-15.
10 Cornejo-Garrido J, Becerril-Chavez F, Carlin-Vargas G et al. Antihyperglycaemic effect of laser acupuncture treatment at BL20 in diabetic rats. Acupunct Med. 2014 ; 32(6) : 486-94.   DOI
11 Lee YC, Li TM, Tzeng CY, et al. Electroacupuncture-induced cholinergic nerve activation enhances the hypoglycemic effect of exogenous insulin in a rat model of streptozotocin-induced diabetes. Exp Diabetes Res. 2011 ; 10(1) : 1-7.
12 Nakamura H, Ishigami T, Kawase Y et al. Effects of acupuncture stimulation on blood glucose concentration in the Otsuka Long-Evans Tokushima Fatty (OLETF) rat, an animal model for type-2 diabetes mellitus. Med Sci Monit Basic Res. 2014 ; 20 : 70-5.   DOI
13 Ahmed D, Kumar V, Verma A et al. Antidiabetic, renal/hepatic/pancreas/cardiac protective and antioxidant potential of methanol/dichloromethane extract of Albizzia Lebbeck Benth. stem bark (ALEx) on streptozotocin induced diabetic rats. BMC Complement Altern Med. 2014 ; 14 : 243   DOI
14 Kim OG, Jeong JC. Effects of the Mori folium Extract in streptozotocin-induced diabetic rats. Koran J Orient Int. 2006 ; 27(4) : 811-21.
15 Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003 ; 17(1) : 24-38.   DOI
16 Adeyemi DO, Ukwenya VO, Obuotor EM, Adewole SO. Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage. BMC Complement Altern Med. 2014 ; 14 : 277.   DOI
17 Al-Malki AL, Sayed AA, El Rabey HA. Proanthocyanidin attenuation of oxidative stress and NF-${\kappa}$B protects apolipoprotein E-deficient mice against diabetic nephropathy. Evid Based Complement Alternat Med. 2013 ; 1(1) : 1-8.
18 Hull GL, Woodside JV, Ames JM, Cuskelly GJ. Validation study to compare effects of processing protocols on measured N (${\varepsilon}$)-(carboxymethyl)lysine and N (${\varepsilon}$)-(carboxyethyl)lysine in blood. 2013 ; 53 (3) : 129-33.   DOI
19 Kim OK, Leem HJ, Je JM, Lee GM. The effect of Meliae toosendan fructus ethanol extract on blood glucose, lipid metabolism, carbohydrate methabolism related enzyme activities and antioxidative effect in streptozotocin-induced diabetic rats. Korean J Oil Chemists' Soc. 2014 ; 31(2) : 227-84.
20 Ko IH. Enact of glutathione(GSH/GSSG) contents of fermented Ginseng on the${\gamma}$-irradiated liver of mice. Kor J. Radiation Therapy. 2006 ; 18(1) : 29-34.
21 Yamagishi S, Matsui T. Advanced glycation end products, oxidative stress and diabetic nephropathy. Oxid Med Cell Longev. 2010 ; 3(2) : 101-8.   DOI
22 Gloire G, Legrand-Poels S, Piette J. NF-kappaB activation by reactive oxygen species: fifteen years later. Biochem Pharmacol. 2006 ; 72(11) : 1493-505.   DOI
23 Kang WS, Kim YC, Lee JH, WOO HJ. The effects of Injinchunggan-tang (Yinchenqinggantang) on TNF-alpha signal transmission system in HepG2 cell. Korean J Orient Int. 2004 ; 25(1) : 28-45.
24 Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappaBalpha phosphorylation, C/EBP and AP-1 activation. Br J Pharmacol. 2003 ; 139(1) : 11-20.   DOI
25 Nilufer Yonguc G, Dodurga Y, Kurtulus A, Boz B, Acar K. Caspase 1, Caspase 3, TNF-alpha, p53, and Hif1-alpha gene expression status of the brain tissues and hippocampal neuron loss in short-term dichlorvos exposed rats. Mol Biol Rep. 2012 ; 39(12) : 10355-60.   DOI
26 Ghosh S, Khazaei M, Moien-Afshari F, et al. Moderate exercise attenuates caspase-3 activity, oxidative stress, and inhibits progression of diabetic renal disease in db/db mice. Am J Physiol Renal Physiol. 2009 ; 296(4) : F700-8.   DOI
27 Daniel E Frances, Paola I Ingaramo, Maria T Ronco, Cristina E Carnovale. Diabetes, an inflammatory process: Oxidative Stress and TNF-alpha involved in hepatic complication. J. Biomedical Science and Engineering. 2013 ; 6 : 645-53.   DOI
28 Statics Korean. Koreanational health and nutrition examination survey. 2013. [cited2014 Sep25] Available from: URL: https://knhanes.cdc.go.kr/knhanes/sub04/sub04_04.do
29 Esposito G, Scuderi C, Lu J et al. S100B induces tau protein hyperphosphorylation via Dickopff-1 up-regulation and disrupts the Wnt pathway in human neural stem cells. J Cell Mol Med. 2008 ; 12(3) : 914-27.   DOI
30 Seo SH, Kim YS, Han YH. The effects of Hirudo on streptozo-tocin-induced diabetic nephropathic rats. Korean J Oriental Med. 2008 ; 29(4) : 133-45.
31 Moon S. Jin WJ, Kim HY, Yang CH, Kim MR. The recent research trends of acupuncture for the treatment of diabetes mellitus. Korean J East-West Med. 2013 ; 38(2) : 13-20.
32 Yu DJ, Yoon GM, Kang SB. Effects of Hwangryungeehwang-tang Aqueous extracts on streptozotocin-induced diabetic rat. Korean J Orient Int Med. 2011 ; 32(2) : 288-300.
33 Kang MK, Nam SS, Lee YH, Kim YS. Effects of CV12 and ST36 acupuncture on glucose metabolism and protection of pancreatic beta cells in neonatal-streptozotocin model. The Acupunct. 2008 ; 25(2) : 91-103.
34 Lee YK, Kang SB. The effects of Gamioryungsan extracts on streptozotocin-induced diabetic nephropathy rats. Korean J Orient Int Med. 2012 ; 33(4) : 367-86.
35 Lee MO, Na CS, Youn DH, Cho MR. Effect of acupuncture at TE4, SP3 and TE4+SP3 in hyperglycemia rats induced by streptozotocin. Korean J Acupuncture. 2007 ; 24(1) : 95-111.
36 Park HJ, Kim JS. Effects of SP3 and GV6 acupuncture on the STZ-treated rats for induction of diabetes. Korean J Electron Mmicroscopy. 2006 ; 36(4) : 279-89.
37 Park JY, Yoo CK, Han JS, Kim HS, Lee YH, Park HS. The effects of acupuncture at Taepo( SP21) on serum glucose and urine glucose in diabetic patient. The Acupunct. 2002 ; 19(1) : 1-10.
38 Oh TW, Kang SY, Park YK. Histological analysis of five organs in streptozotocin-induced diabetic rats. Korean J Herbology. 2013 ; 28(6) : 39-45.
39 van Belle TL, Coppieters KT, von Herrath MG. Type1 diabetes: etiology, immunology, andtherapeutic strategies. Physiol Rev. 2011 ; 91(1) : 79-118.   DOI
40 Koo ST, Kim SK, Kim EH et al. Acupuncture Point Locations for Experimental Animal Studies in Rats and Mice. Korean J Acupuncture. 2010 ; 27(3) : 67-78.