Browse > Article
http://dx.doi.org/10.13045/acupunct.2016031

Effect of Gentianae Macrophyllae Radix Pharmacopuncture at Hwando (GB30) on Neuropathic Pain in Tibial and Sural Nerve Transected Rats  

Lee, Ook Jae (Cham Joeun Hospital)
Kim, Seon Wook (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University)
Shin, Jeong Cheol (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Dongshin University)
Publication Information
Journal of Acupuncture Research / v.33, no.3, 2016 , pp. 1-16 More about this Journal
Abstract
Objectives : The objective of this study was to investigate the effects of Gentianae Macrophyllae Radix pharmacopuncture (GP) at Hwando (GB30) in neuropathic pain induced rats. Methods : Neuropathic pain in rats was induced by tibial and sural nerve transection. The rat subjects were divided into 6 groups : normal (Nor, n = 5), control (Con, n = 5), neuropathic pain- induced injected at GB30 with 1 mg/kg GP (GP-A, n = 5), 5 mg/kg GP (GP-B, n = 5) and 20 mg/kg GP (GP-C, n = 5), and neuropathic pain-induced injected with 1mg/kg Tramadol (Tramadol, n＝5). Injections were administered 2 times a week for a total of 5 treatments. After each treatment plantar withdrawal response was measured and after all 5 treatments were completed c-fos, Bax, Bcl-2, mGlu5 and leukocytes in the blood were analyzed. Results : 1. Groups GP-A, GP-B and GP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia compared to the control group. 2. Groups GP-A, GP-B and GP-C showed a meaningful decrease in the expression of c-fos compared to the control group. 3. Groups GP-A and GP-C showed a meaningful increase in the expression of mGluR5 compared to the control group. 4. Groups GP-A, GP-B and GP-C showed a meaningful decrease in Bax/Bcl-2 ratio compared to the control group. Conclusion : These results suggest that Gentianae Macrophyllae Radix pharmacopuncture at Hwando (GB30) could decrease mechanical allodynia and could have analgesic and neuroprotective effects on the model of neuropathic pain.
Keywords
Neuropathic pain; Gentianae Macrophyllae Radix; Pharmacopuncture; Hwando (GB30);
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Zhaoa L, Jiaoa Q, Yanga P et al. Metabotropic glutamate receptor 5 promotes proliferation of human neural stem/progenitor cells with activation of mitogen-activated protein kinases signaling pathway in vitro. Neuroscience. 2011 ; 192 : 185-94.   DOI
2 Maiese K, Swiriduk M, TenBroeke M. Cellular Mechanisms of Protection by Metabotropic Glutamate Receptors During Anoxia and Nitric Oxide Toxicity. J Neurochem. 1996 ; 66(6) : 2419-28.
3 Chiang SY, Bang SP, Kim JH, Wei TS, Yun YC. Effects of Bee Venom Acopuncture at Sinsu(BL23) and Daejangsu(BL25) in Different Concentration on Neuropathic Pain Induced by Tibial and Sural Nerve Injury in Rats. The Acupunct. 2010 ; 27(5) : 13-24.
4 Lee CR, McTavish D, Sorkin EM. Tramadol. Drugs. 1993 ; 46(2) : 313-40.   DOI
5 Jensen TS, Baron R, Haanpaa M et al. A new definition of neuropathic pain. Pain. 2011 ; 152 : 2204-5.   DOI
6 Maag R, Baron R. Neuropathic pain : translational research and impact for patient care. Curr Pain Headache Rep. 2006 ; 10(3) : 191-8.   DOI
7 Kim SS, Ko SG, Cho KH et al. East-West Medical Study on Bi syndrome-Focusing on Causes and Symptoms. Korean J.Orient.Int. Med. 1994 ; 15(1) : 116-27.
8 Minn YK, Kim SM. Diagnosis and Treament of Neuropathic Pain. J Korean Med Assoc. 2008 ; 51(12) : 1139-48.   DOI
9 van Hecke O, Austin SK, Khan RA, Smith BH, Torrance N. Neuropathic pain in the general population : a systematic review of epidemiological studies. Pain. 2014 ; 155(4) : 654-62.   DOI
10 Lee KW. Huangdi Neijing Suwen 1. Seoul : Yeogang Press. 2007 : 581-2, 644-5.
11 The Compilation Committee of the Korean Acupuncture and Moxibustion Society. The Acupuncture and Moxibustion (2). Gyeonggi : Jipmoondang. 2008 : 408.
12 Kim SP, Choi SM, Kim JE, Kim JH, Shin KM, Kim JH. Effects of Clematidis Radix Pharmacopuncture Injected at Sinsu(BL23) on Neuropathic Pain in Rats. The Acupunct. 2014 ; 31(4) : 57-70.
13 Lee DG, Lee OJ, Lee JH et al. The Effects of Eucommiae Cortex Pharmacopuncture Injected at Sinsu(BL23) on Neuropathic Pain in Rats. The Acupunct. 2013 ; 30(4) : 69-78.   DOI
14 Bang SP, Ryu MS, Kim JH et al. Effects of Radix Aconiti Herbal Acupuncture Injected at Hwando(GB30) on Neuropathic Pain in Rats. The Acupunct. 2009 ; 26(6) : 67-76.
15 Lee DH, Youn DH, Na CS, Yoon YC. Effects of Bee Venom Acupuncture Injected at Hwando (GB30) on Neuropathic Pain in Rats. The Acupunct. 2005 ; 22(5) : 67-77.
16 Kim SN, Kim SC, Choi HK et al. Inhibitory Effect of Scolopendird Aqua-Acupuncture Injected at Hwando(GB30) on Neuropathic Pain in Rats. The Acupunct. 2004 ; 21(3) : 145-67.
17 Kim SJ, Jung MJ, Park SH et al. Analgesic Effects of Herba Chelidonii Pharmacopuncture in the Rat Models of Peripheral Neuropathic Pain. J Oriental Rehab Med. 2007 ; 17(2) : 1-16.
18 Park JH, Yim YK, Lee H. A Study on the Effect of Herbal-acupuncture with Gentianae macrophyllase Radix Solution at Joksamni(ST36) on Collagen-induced Arthritis DBA/1J mice. Korean Journal of Acupuncture. 2007 ; 24(3) : 117-37.
19 Korean Medicine Univ Herbalogy Compilation Committee. Herbalogy. Seoul : Younglimsa. 2004 : 310-1.
20 Cho HC, Jung HJ, Lee JG, Jo MG, Jee SY. Antiinflammaory effects of the MeOH extract of Gentianae Macrophyllae Radix in vivo. J Korean Med Ophthalmol Otolaryngol Dermatol. 2009 ; 22(3) : 63-70.
21 Korean Medicine Univ Acupuncture & Moxibustion and Acupoint Department. The Acupuncture and Moxibustion 1. Seoul : Jipmoondang. 1998 : 643-5.
22 Je BS, Lim LC, Oh MS. The Literatural Study on Acupuncture and Moxibustion Therapy of Arthalgia Syndrome. Research Institute of Korean Medicine Thesis Collection. 2004 ; 13(1) : 61-9.
23 Butera JA. Current and emerging targets to treat neuropathic pain. J Med Chem. 2007 ; 50(11) : 2543-6.   DOI
24 Nickel FT, Seifert F, Lanz S, Maihofner C. Mechanisms of neuropathic pain. Eur Neuropsychopharmacology. 2012 ; 22 : 81-91.   DOI
25 Jongen JL, Hans G, Benzon HT, Huygen F, Hartrick CT. Neuropathic pain and pharmacological treatment. Pain Pract. 2014 ; 14(3) : 283-95.   DOI
26 Honore PH, Basnet A, Laila Eljaja L et al. Neuropathic pain models in the development of analgesic drugs. Scandinavian Journal of Pain. 2011 ; 2(4) : 172-7.   DOI
27 Bennett GJ, Xie YK. A peripheral mononeuropathy in rats that produces disorders of pain sensation like those seen in man. Pain. 1988 ; 33 : 87-107.   DOI
28 Lee BH, Baik EJ, Lee SH, Moon CH. An animal model of neuropathic pain employing injufy to the sciatic nerve branches. Neuroreport. 2000 ; 11(4) : 657-61.   DOI
29 Seltzer Z, Dubner R, Shir Y. A novel behavioral model of neuropathic pain disorders produced in rats by partial sciatic nerve injury. Pain. 1990 ; 43 : 205-18.   DOI
30 Kim SH, Chung JM. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain. 1992 ; 50 : 355-63.   DOI
31 Dowdall T, Robinson I, Meert TF. Comparison of five different rat models of peripheral nerve injury. Pharmacol Biochem Behav. 2005 ; 80(1) : 93-108.   DOI
32 Scientific Committee of Korean Pharmacopuncture Institute. Pharmacopuncturology. Seoul : Elsevier Korea. 2008 : 3, 6-8.
33 Deadman P, Al-Khafaji M, Baker K. A Manual of Acupuncture. China : Journal of Chinese Medicine Publications. 2007 : 446-7.
34 Barber J. Examining the use of tramadol hydrochloride as an antidepressant. Exp Clin Psychopharmacol. 2011 ; 19(2) : 123-30.   DOI
35 Nirogi R, Goura V, Shanmuganathan D, Jayarjan P, Abraham R. Comparison of manual and automated filaments for evaluation of neuropathic pain behavior in rats. J Pharmacol Toxicol Methods. 2012 ; 66 : 8-13.   DOI
36 Hunt SP, Pini A, Evan G. Induction of c-foslike protein in spinal cord neurons following sensory stimulation. Nature. 1987 ; 328 : 632-4.   DOI
37 Kim HK, Kim PJ, Baik SW, Kim IS, Chung KS. Molecular Biologic Study on tbe Changes of Glutamate Receptor (mGluR5) in Rat Hippocampus after Brain Ischemia. Korean J Crit Care Med. 2000 ; 15(2) : 75-81.
38 Harris JA. Using c-fos as a neural marker of pain. Brain Res Bull. 1998 ; 45(1) : 1-8.   DOI
39 Coggeshall RE. Fos, nociception and the dorsal horn. Prog Neurobiol. 2005 ; 77(5) : 299-352.   DOI
40 Choi JH. Bax protein in cancer treatment. J Korean Med Assoc. 2007 ; 50(11) : 1016-22.   DOI