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http://dx.doi.org/10.13045/acupunct.2013007

The Effects of Pharmacopuncture(Eugenia caryophyllata $T_{HUNB.}$) on the High Fat Diet-induced Obese ICR Mice  

Kim, Sung Jin (Department of Acupuncture & Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
Lee, Hyun Jong (Department of Acupuncture & Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
Lee, Bong Hyo (Department of Acupuncture & Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
Lee, Yun Kyu (Department of Acupuncture & Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
Lim, Seong Chul (Department of Acupuncture & Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
Jung, Tae Young (Je-Han Oriental Medical Academy)
Kim, Jae Soo (Department of Acupuncture & Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
Publication Information
Journal of Acupuncture Research / v.30, no.3, 2013 , pp. 75-85 More about this Journal
Abstract
Objectives : The purpose on this study is to show the anti-obesity effect of Eugenia caryophyllata $T_{HUNB.}$ pharmacopuncture in mice fed a high fat diet. Methods : Male ICR mice were divided into three groups, which were fed a normal AIN diet(N), a high fat diet(CT), a high fat diet and injected with 50 mg/kg of Eugenia caryophyllata $T_{HUM.}$ pharmacopuncture(EC) for 8 weeks. Results : 1. The levels of body weight gains were significantly increased in CT compared with N and decreased in EC compared with CT. 2. The levels of absolute liver weight and adipose-tissue weight were significantly decreased in EC compared with CT. The levels of relative adipose-tissue weight were significantly decreased in EC compared with CT. 3. The levels of total cholesterol(TG), triglycerid(TC), high density lipoprotein(HDL) cholesterol and low density lipoprotein(LDL) cholesterol were significantly increased in CT compared with N and decreased in EC compared with CT. The levels of HDL/LDL ratio were shown there were no significant differences in all groups. 4. The levels of adiponectin in serum were remarkably decreased in CT compared with N and significantly increased in EC compared with CT. 5. The levels of measuring adipocyte number in adipose tissue was significantly decreased in CT compared with N and increased in EC compared with CT. Conclusions : These results suggest that Eugenia Caryophyllata $T_{HUNB.}$ has an anti-obesity effect in mice and that the effect is mediated by inhibition of fat gain.
Keywords
Eugenia caryophyllata $T_{HUNB.}$; Pharmacupuncture; Obesity;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 Lee YK. Identification and antimicrobial activity of cinnamon and clove extracts on food spoilage microorganisms[dissertation]. Seoul : Sookmyung University. 1995. Korean.
2 Leem HH, Kim EO, Seo MJ, Choi SW. Antioxidant and anti-inflammatory activites of eugenol and its derivatives from clove(eugenia caryophyllata thunb.). Kor J Soc Food Sci Nutr. 2011 ; 40(10) : 1361-70.
3 Kwon GS. Antifungal Activity of Eugenia caryophyllata extracts and identification of antifungal compounds[dissertation]. Seoul : Seoul National University of Technology. 2006. Korean.
4 Dong Suk, Jung SH, Moon JS, Rhee SK, Son JY. Antioxidant Activities of Clove by Extraction Solvent. Kora J Soc Food Sci Nutr. 2004 ; 33(4) : 609-13.   DOI
5 Lee OH, Jing SH, Son JY. Antimicrobial Activity of Clove Extract by Extraction Solvents. Kora J Soc Food Sci Nutr. 2004 ; 33(3) : 494-9.
6 Yang YY, Lee MJ, Lee HS, Park WH. Screening of antioxidative, anti-platelet aggregation and anti-thrombotic effects of clove extracts. Kor J Oriental Physiology and Pathology. 2011 ; 25(3) : 471-81.
7 Considine RV, Sinha MK, Heiman ML et al. Serum immunoreactive-leptin concentrations in normal-weight and obese mans. N Engl J Med. 1996 ; 334(5) : 292-5.   DOI   ScienceOn
8 Jung YS, Yoon KH, Choi SB, Yoon MC, Shin SS. Clinical efficacy of gyeongshingangjeehwan16 according to obesity related to measurement variables. Kor J Oriental Medical Prescription. 2008 ; 16(1) : 169-83.
9 Kim KW, Chung WS, Chung SH. A clinical case study on the changes of body composition and blood chemistry after modified fasting therapy in a patient with obesity. Journal of Society of Korean Medicine for Obesity Research. 2010 ; 10(1) : 49-56.
10 Kim HK, Kim MK. Effects of weight control program on dietary habits and blood composition in obese middle-aged women. Kor J Nutr. 2010 ; 43(3) : 273-84.   DOI
11 Kwak NS, Kim Eojina, Kim HR. Current status and improvements of obesity related legislation. Kor J Nutr. 2010 ; 43(4) : 413-23.   DOI
12 Zheng GQ, Kenney PM, Lam LKT. Sesquiterpenes from clove(Eugenia caryophyllata). J Nat Prod. 1992 ; 55(7) : 999-1003.   DOI
13 Kim MW, Song YK, Lim HH. Study of experimentations and clinical trials' trends for obesity treatment using pharmacupuncture. Kor J Medicine for Obesity Research. 2011 ; 11(1) : 47-60.
14 Herbology textbook publish commission. Herbology. Soeul : Yunglimsa. 2010 : 383-4.
15 Chami F, Chami N, Bennis S, Bouchikhi T, Remmal A. Oregano and clove essential oils induce surface alteration of Saccharomyces cerevisiae. Phytother Res. 2005 ; 19(5) : 405-8.   DOI   ScienceOn
16 Kim HM, Lee EH, Hong SH et al. Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats. J Ethnopharmacol. 1998 ; 60(2) : 125-31.   DOI   ScienceOn
17 Miyazawa M, Hisama M. Suppression of chemical mutagen induced SOS response by alkylphenols from clove(Syzygium aromaticum) in Salmonella typhymurium TA1535/pSK1002 umu test. J Agric Food Chem. 2001 ; 49(8) : 4019-25.   DOI   ScienceOn
18 Ogata M, Hoshi M, Urano S, Endo T. Antioxidant activity of eugenol and related monomeric and dimeric compounds. Chem Pharm Bull. 2000 ; 48(10) : 1467-9.   DOI   ScienceOn
19 Park IK, Lee HS, Lee SG, Park JD, Ahn YJ. Insecticidal and fumigant activities of Cinnamomum cassia bark-derived materials against Mechoris ursulus (Coleoptera: Attelabidae). J Agric Food Chem. 2000 ; 48(6) : 2528-31.   DOI   ScienceOn
20 Kamel Chaieb, Hafedh Hajlaoui, Tarek Zmantar et al. The Chemical Composition and Biological Activity of Clove Essential Oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): A Short Review. Phytother Res. 2007 ; 21(6) : 501-6.   DOI   ScienceOn
21 Kim SK, Kim YM, Homg MJ, Rhee HI. Study on the inhibitory effect of Eugenia aromaticum extract on pancreatic lipase. Agric Chem Biotechnol. 2005 ; 48(2) : 84-8.
22 Lee YS, Kim JH, Kim HJ et al. Anti-lipase and lipolytic activities of EtOH extract from juniperus rigida. Kor J Pharmacogn. 2010 ; 41(3) : 216-20.
23 Kim DH, Yang BS. Molecular mechanisms underlying the link of obesity to chronic diseases. Kor J Oriental Association for Study of Obesity. 2006 ; 15(3) : 121-8.
24 Kissebah AH, Uydelingum N, Mrray F, Evans DJ & Hartz AJ. Realation of body-fat distribution to metabolic complications of obesity. J Clin Endocrinol Met. 1982 ; 54(2) : 254-60.   DOI
25 Shin SW, Kim KS. Changes in of abdominal subcutaneous and visceral fatfollowingafter abdominal obesity treatment. Kor J Oriental Association for study of obesity. 2006 ; 6(2) : 95-104.
26 Cha SY, Jang JY, Lee YH et al. Lipolytic Effect of methanol extracts from luffa cylindrica in mature 3T3-L1 adipocytes. Kor J Soc Food Sci Nutr. 2010 ; 39(6) : 813-9.
27 Bae BC. Gm-Taek Huangdi's Canon of Medicine Plain Questions. Seoul : Seongbosa. 1994 :
28 Shin HD. Basic concepts and treatment methods of obesity in oriental medicine. Kyung Hee Oriental Medicine. 1995 ; 1(1) : 257-67.
29 Cho SH, Lee JS, Thabane L, Lee J. Acupuncture for obesity: a systematic review and meta-analysis. International of Obesity. 2009 ; 33(2) : 183-96.   DOI
30 Hwang DS, Ahn SJ, Lee KS. Effect of electroacupuncture on regional fat by measuring thigh circumference. Kor J Oriental Association for Study of Obesity. 2005 ; 5(1) : 1-8.
31 Shin SU, Kim KS. Changes in of abdominal subcutaneous and visceral fatfollowingafter abdominal obesity treatment. Kor J Oriental Association for Study of Obesity. 2006 ; 6(2) : 95-104.
32 Jang HJ, Lee HS, Lee JM. Effect of Hagocho (Prunella vulgaris L.), Gamgook(Chrysanthemum indicum L.) and Galaeun(pueraria Radix) aquaacupuncture at Gokji(LI-11) and Joksamri (ST-36) on lowering lipid effect, oxodative capacity, concentration of TNF-a, IL-6, Leptin and histological consideration in hyperlipidemic rat. Kor J of Meridian & Acupoint. 2007 ; 24(4) : 201-19.
33 Youh EJ, Seo BK, Huang Bo, Kim JI, Kang SK. Artemisia capillaries herbal acupuncture improves metabolic abnormalities in high fat diet-induced obese ICR mice. Kor J Acupuncture & Moxibustion Society. 2012 ; 28(1) : 2-14.
34 Kwon Kang, Park HS. The effects of lumbricus pharmacopuncture on the lipid lowering, anti-oxidative activity and concentration of proinflammatory cytokines in rat fed high fat diet. Kor J Acupuncture & Moxibustion Society. 2010 ; 27(2) : 59-69.
35 Youh EJ, Seo BK, Nam SS, Knag SK. Antiobesity effect of rhizoma atractylodis herbal acupuncture in high fat diet-induced obese ICR mouse. Kor J Acupuncture & Moxibustion Society. 2010 ; 27(6) : 31-42.
36 Lim CS, Park WP, Jang SB et al. Clinical Studies of Sweet Bee Venom to The Effect of Abdominal Fat Accumulation. Journal of Pharmacopuncture. 2008 ; 11(2) : 33-40.
37 Cha YY, Kim YS, Shin YW, Kwon KR, Lee SG. A clinical study for the influence of herbai acupuncture therapy(Sangsik no.1) on Obesity. Jor of Pharmacopuncture. 2001 ; 4(2) : 57-63.   DOI
38 Chami F, Chami N, Bennis S, Bouchikhi T, Remmal A. Oregano and clove essential oils induce surface alteration of Saccharomyces cerevisiae. Phytother Res. 2005 ; 19(5) : 405-8.   DOI   ScienceOn
39 Lee HS. Antibacterial activityand antioxidant activity of syzygium aromaticum(Clove) extracts [dissertation]. Naju : Dongshin University. 2005. Korean.
40 Dong Suk. Physiological activities of clove by the different extraction solvents[dissertation]. Anseong : Hankyong University. 2004. Korean.