Browse > Article
http://dx.doi.org/10.5714/CL.2017.22.042

Graphene accelerates osteoblast attachment and biomineralization  

Ren, Jia (School of Mechanical and Electric Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)
Zhang, Xiaogang (School of Mechanical and Electric Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)
Chen, Yao (School of Mechanical and Electric Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University)
Publication Information
Carbon letters / v.22, no., 2017 , pp. 42-47 More about this Journal
Abstract
In this paper, the in vitro biocompatibility of graphene film (GF) with osteoblasts was evaluated through cell adhesion, viability, alkaline phosphatase activity, F-actin and vinculin expressions, versus graphite paper as a reference material. The results showed that MG-63 cells exhibited stronger cell adhesion, better proliferation and viability on GF, and osteoblasts cultured on GF exhibited vinculin expression throughout the cell body. The rougher and wrinkled surface morphology, higher elastic modulus and easy out-of-plane deformation associated with GF were considered to promote cell adhesion. Also, the biomineralization of GF was assessed by soaking in simulated body fluid, and the GF exhibited enhanced mineralization ability in terms of mineral deposition, which almost pervaded the entire GF surface. Our results suggest that graphene promotes cell adhesion, activity and the formation of bone-like apatite. This research is expected to facilitate a better understanding of graphene-cell interactions and potential applications of graphene as a promising toughening nanofiller in bioceramics used in load-bearing implants.
Keywords
graphene film; osteoblast; adhesion; viability; mineralization;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lee C, Wei X, Kysar JW, Hone J. Measurement of the elastic properties and intrinsic strength of monolayer graphene. Science, 321, 385 (2008). https://doi.org/10.1126/science.1157996.   DOI
2 Huang BR, Chan HW, Jou S, Chen GY , Kuo HA, Song WJ. Structure and field emission of graphene layers on top of silicon nanowire arrays. Appl Surf Sci, 362, 250 (2016). https://doi.org/10.1016/j.apsusc.2015.11.256.   DOI
3 Frank IW, Tanenbaum DM, Van der Zande AM, McEuen PL. Mechanical properties of suspended graphene sheets. J Vac Sci Technol B Nanotechnol Microelectron, 25, 2558 (2007). https://doi.org/10.1116/1.2789446.   DOI
4 Steurer P, Wissert R, Thomann R, Mülhaupt R. Functionalized graphenes and thermoplastic nanocomposites based upon expanded graphite oxide. Macromol Rapid Commun, 30, 316 (2009). https://doi.org/10.1002/marc.200800754.   DOI
5 Zhan GD, Kuntz JD, Wan JL, Mukherjee AK. Single-wall carbon nanotubes as attractive toughening agents in alumina-based nanocomposites. Nat Mater, 2, 38 (2003). https://doi.org/10.1038/nmat793.   DOI
6 Rafiee MA, Rafiee J, Wang Z, Song H, Yu ZZ, Koratkar N. Enhanced mechanical properties of nanocomposites at low graphene content. ACS Nano, 3, 3884 (2009). https://doi.org/10.1021/nn9010472.   DOI
7 Kun P, Tapaszto O, Wéber, F, Balazsi C. Determination of structural and mechanical properties of multilayer graphene added silicon nitride-based composites. Ceram Int, 38, 211 (2012). https://doi.org/10.1016/j.ceramint.2011.06.051.   DOI
8 Kvetkova L, Duszova A, Hvizdos P, Dusza J, Kun P, Balazsi C. Fracture toughness and toughening mechanisms in graphene platelet reinforced Si3N4 composites. Scr Mater, 66, 793 (2012). https://doi.org/10.1016/j.scriptamat.2012.02.009.   DOI
9 Lahiri D, Ghosh S, Agarwal A. Carbon nanotube reinforced hydroxyapatite composite for orthopedic application: a review. Mater Sci Eng C, 32, 1727 (2012). https://doi.org/10.1016/j.msec.2012.05.010.   DOI
10 Walker LS, Marotto VR, Rafiee MA, Koratkar N, Corral EL. Toughening in graphene ceramic composites. ACS Nano, 5, 3182 (2011). https://doi.org/10.1021/nn200319d.   DOI
11 Zhang L, Liu W, Yue C, Zhang T, Li P, Xing Z, Chen Y. A tough graphene nanosheet/hydroxyapatite composite with improved in vitro biocompatibility. Carbon, 61, 105 (2013). https://doi.org/10.1016/j.carbon.2013.04.074.   DOI
12 Zhang L, Zhang XG, Chen Y, Su JN, Liu WW, Zhang TH, Qi F, Wang YG. Interfacial stress transfer in a graphene nanosheet toughened hydroxyapatite composite. Appl Phys Lett, 105, 161908 (2014). https://doi.org/10.1063/1.4900424.   DOI
13 Chen Y, Zhang YQ, Zhang TH, Gan CH, Zheng CY, Yu G. Carbon nanotube reinforced hydroxyapatite composite coatings produced through laser surface alloying. Carbon, 44, 37 (2006). https://doi.org/10.1016/j.carbon.2005.07.011.   DOI
14 Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic $A-W^{3}$. J Biomed Mater Res Part A, 24, 721 (1990). https://doi.org/10.1002/jbm.820240607.   DOI
15 Jankovic A, Erakovic S, Mitric M, Matic IZ, Juranic ZD, Tsui GCP, Tang CY, Miskovic-Stankovic V, Rhee KY, Park SJ. Bioactive hydroxyapatite/graphene composite coating and its corrosion stability in simulated body fluid. J Alloys Compd, 624, 148 (2015). https://doi.org/10.1016/j.jallcom.2014.11.078.   DOI
16 Liu Y, Dang Z, Wang Y, Huang J, Li H. Hydroxyapatite/graphenenanosheet composite coatings deposited by vacuum cold spraying for biomedical applications: inherited nanostructures and enhanced properties. Carbon, 67, 250 (2014). https://doi.org/10.1016/j.carbon.2013.09.088.   DOI
17 Kalbacova M, Broz A, Kong J, Kalbac M. Graphene substrates promote adherence of human osteoblasts and mesenchymal stromal cells. Carbon, 48, 4323 (2010). https://doi.org/10.1016/j.carbon.2010.07.045.   DOI
18 Aryaei A, Jayatissa AH, Jayasuriya AC. The effect of graphene substrate on osteoblast cell adhesion and proliferation. J Biomed Mater Res Part A, 102, 3282 (2014). https://doi.org/10.1002/jbm.a.34993.   DOI
19 Matsuoka M, Akasaka T, Totsuka Y, Watari F. Strong adhesion of Saos-2 cells to multi-walled carbon nanotubes. Mater Sci Eng B, 173, 182 (2010). https://doi.org/10.1016/j.mseb.2009.12.044.   DOI
20 Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R. Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics. J Biomed Mater Res Part A, 51, 475 (2000). https://doi.org/10.1002/1097-4636(20000905)51:3<475::AIDJBM23>3.0.CO;2-9.   DOI
21 Discher DE, Janmey P, Wang YL. Tissue cells feel and respond to the stiffness of their substrate. Science, 310, 1139 (2005). https://doi.org/10.1126/science.1116995.   DOI
22 Deepachitra R, Chamundeeswari M, Kumar BS, Krithiga G, Prabu P, Devi MP, Sastry TP. Osteo mineralization of fibrin-decorated graphene oxide. Carbon, 56, 64 (2013). https://doi.org/10.1016/j.carbon.2012.12.070.   DOI
23 Palchesko RN, Zhang L, Sun Y, Feinberg AW. Development of Polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve. PLoS One, 7, e51499 (2012). https://doi.org/10.1371/journal.pone.0051499.   DOI