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http://dx.doi.org/10.9799/ksfan.2022.35.3.204

Effects of Fucoidan, a Sulfur-Containing Polysaccharide, on Cytotoxicity and Apoptosis in HT-29 Human Colorectal Cancer Cells  

Kim, Min Ji (Dept. of Food and Nutrition, College of Science and Technology, Duksung Women's University)
Chung, Ha Sook (Dept. of Food and Nutrition, College of Science and Technology, Duksung Women's University)
Publication Information
The Korean Journal of Food And Nutrition / v.35, no.3, 2022 , pp. 204-212 More about this Journal
Abstract
The purpose of this study was to investigate the biological activity of fucoidan, a sulfur-containing polysaccharide, on cytotoxicity and apoptosis in the human HT-29 colorectal cancer cell line using cell viability, Flow cytometry, Western blot, and RT-PCR analyses. Fucoidan inhibited the proliferation of HT-29 cells by 39.6% at a concentration of 100 ㎍/mL for 72 h. The inhibition was dose-dependent and accompanied by apoptosis. Flow cytometric analysis showed that fucoidan increased early apoptosis and late apoptosis by 65.84% and 72.09% at concentrations of 25 and 100 ㎍/mL, respectively. Analysis of the mechanism of these events indicated that fucoidan-treated cells exhibited increases in the activation of caspase-3, caspase-8, and PARP in a dose-dependent manner. These results suggest that fucoidan may inhibit the growth of human colorectal cancer cells by various apoptosis-promoting effects, as well as by apoptosis itself.
Keywords
fucoidan; sulfur-containing polysaccharide; apoptosis; colorectal cancer; HT-29;
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