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http://dx.doi.org/10.9799/ksfan.2021.34.5.477

Evaluation of the Physiological Activity and Identification of the Active Ingredients of Crab Apple (Malus prunifolia Borkh.) Extracts  

Shin, Hyun Young (Transdisciplinary Major in Learning Health System, Department of Integrated Biomedical and Life Science, Korea University)
Kim, Hoon (College of Biotechnology and Natural Resources, Chung-Ang University)
Jeong, Eun-Jin (Major in Food and Nutrition, Korea National University of Transportation)
Kim, Hyun-Gyeong (Major in Food and Nutrition, Korea National University of Transportation)
Lee, Kyung-Haeng (Major in Food and Nutrition, Korea National University of Transportation)
Bae, Yun-Jung (Major in Food and Nutrition, Korea National University of Transportation)
Kim, Woo Jung (Biocenter, Gyeonggido Business and Science Accelerator)
Lee, Sanghyun (Dept. of Plant Science and Technology, Chung-Ang University)
Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
Publication Information
The Korean Journal of Food And Nutrition / v.34, no.5, 2021 , pp. 477-486 More about this Journal
Abstract
To utilize Malus pruniforia Borkh. as a functional material, cold-water (CW), hot-water (HW), and 70% ethanol (EtOH) extracts were prepared, and their antioxidant and anti-inflammatory activities were compared. The antioxidant activity of the HW extract evaluated by ABTS and DPPH radical scavenging and FRAP activity was significantly effective. The total polyphenol content of the HW extract was also higher by 15.5±0.7 mg GAE/g extract compared to other extracts. The EtOH extract showed significantly decreased TNF-α (39.8%), IL-6 (65.5%), and NO (34.9%) levels in RAW 264.7 cells compared to the LPS-induced control group. The levels of IL-6 (21.1%) and IL-8 (19.3%) were significantly decreased by treatment of EtOH extract in HaCaT keratinocytes induced with TNF-α and IFN-γ. The UHPLC-MS results indicated that the EtOH extract might have chlorogenic acid and phlorizin as the major compounds. This was validated using HPLC-DAD, which showed that the EtOH extract had higher levels of chlorogenic acid and phlorizin (1,185±58 and 470±10 ㎍/g extract, respectively). In conclusion, the present study suggested that the anti-inflammatory activity of the EtOH extract was more effective than the CW and HW extracts, and chlorogenic acid and phlorizin could be used as indicator compounds and functional substances.
Keywords
Malus prunifolia Borkh.; solvent extract; antioxidant; anti-inflammatory activity; indicator compound;
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1 Kim YH. 1999. Stabilities of anthocyanin pigmenta obtained from crab apple (Malus prunifolia Wild. Borkh. "red fruit") by ethanol extraction. Korean J Food Nutr 12:85-90
2 Kweon HJ, Park MY, Song YY, Son KM, Sagong DH. 2016. Influence of temperature and plant growth regulators on pollen germination and pollen tube growth of apple cultivars bred in Korea. Prot Hortic Plant Fact 25:184-192   DOI
3 Lee EH, Park HJ, Kim BO, Choi HW, Park KI, Kang IK, Cho YJ. 2020. Anti-inflammatory effect of Malus domestica cv. Green ball apple peel extract on RAW 264.7 macrophages. J Appl Biol Chem 63:117-123   DOI
4 Lee KH, Yoon Y, Kwon HW, Lee EH. 2018. Antioxidant component and activity of different part extracts in apple (Malus domestica cv. Fuji). Korean J Food Nutr 31:858-864   DOI
5 Lee Y, Ryu M. 2020. A study on the health functional food purchase intentions of Chinese consumers in their 20s and 30s: Focusing on extended model of goal-directed behavior. J Eurasian Stud 17:41-69   DOI
6 Li M, Xue S, Tan S, Qin X, Gu M, Wang D, Zhang Y, Guo L, Huang F, Yao Y, Zhou Z, Fan S, Huang C. 2016. Crabapple fruit extracts lower hypercholesterolaemia in high-fat diet-induced obese mice. J Funct Foods 27:416-428   DOI
7 Li N, Shi J, Wang K. 2014. Profile and antioxidant activity of phenolic extracts from 10 crabapples (Malus wild species). J Agric Food Chem 62:574-581   DOI
8 Razgonova MP, Zakharenko AM, Derbush IV, Sabitov AS, Golokhvast KS. 2021. Research of 5 extracts of wild Amur grape Vitis amurensis Rupr. and identification of its polyphenolic composition by tandem mass spectrometry (HPLC-MS/MS). E3S Web Conf 254:01012
9 Marinova D, Ribarova F, Atanassova M. 2005. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Technol Metall 40:255-260
10 Hwang KC, Shin HY, Kim WJ, Seo MS, Kim H. 2021. Effects of a high-molecular-weight polysaccharides isolated from Korean persimmon on the antioxidant, anti-inflammatory, and antiwrinkle activity. Molecules 26:1600   DOI
11 Reddy MC, Subhashini J, Mahipal SVK, Bhat VB, Reddy PS, Kiranmai G, Madyastha KM, Reddanna P. 2003. C-phycocyanin, a selective cyclooxygenase-2 inhibitor, induces apoptosis in lipopolysaccharide-stimulated RAW 264.7 macrophages. Biochem Biophys Res Commun 304:385-392   DOI
12 Sun L, Guo Y, Fu C, Li J, Li Z. 2013. Simultaneous separation and purification of total polyphenols, chlorogenic acid and phlorizin from thinned young apples. Food Chem 136:1022-1029   DOI
13 Shin HY, Kim H, Jeong EJ, Kim JE, Lee KH, Bae YJ, Yu KW. 2020. Bioactive compounds, anti-oxidant activities and anti-inflammatory activities of solvent extracts from Centella asiatica cultured in Chungju. Korean J Food Nutr 33:692-701   DOI
14 Son KM, Choi DG, Kwon SI, Kim BO, Choi C, Kang IK. 2013. Selection of crabapple pollinizers for 'Fuji' apple through physiological and genetic analysis. Prot Hortic Plant Fact 22:116-122   DOI
15 Stojiljkovic D, Arsic I, Tadic V. 2016. Extracts of wild apple fruit (Malus sylvestris (L.) Mill., Rosaceae) as a source of antioxidant substances for use in production of nutraceuticals and cosmeceuticals. Ind Crops Prod 80:165-176   DOI
16 Hong JJ, Seol HG, Oh JY, Jeong EH, Chang YH. 2021. Effective component contents and antioxidative activities of unripe apple by extraction methods. Korean J Food Nutr 34:174-180   DOI
17 Cho KH, Kim JH, Lee JW, Kwon SI, Park JT, Shin IS, Kim SH, Kim DH, Choi IM. 2014. Identification of self-incompatibility genotypes in apple and crabapple cultivars by S-allele specific PCR analysis. Korean J Breed Sci 46:364-371   DOI
18 Ha Y, Lee WH, Jeong JW, Park M, Ko JY, Kwon OW, Lee J, Kim YJ. 2020. Pyropia yezoensis extract suppresses IFN-gamma- and TNF-alpha-induced proinflammatory chemokine production in HaCaT cells via the downregulation of NF-κB. Nutrients 12:1238   DOI
19 Ha YM, Shim KK. 1995. Selection of new crabapple cultivars as pollinizers for apple orchard. J Korean Soc Hortic Sci 36:281-291
20 Hsieh YHP, Ofori JA. 2007. Innovations in food technology for health. Asia Pac J Clin Nutr 1:65-73
21 Willems JL, Khamis MM, Mohammed Saeid W, Purves RW, Katselis G, Low NH, El-Aneed A. 2016. Analysis of a series of chlorogenic acid isomers using differential ion mobility and tandem mass spectrometry. Anal Chim Acta 933:164-174   DOI
22 Jeon YJ, Han SH, Lee YW, Lee M, Yang KH, Kim HM. 2000. Dexamethasone inhibits IL-1-β gene expression in LPS-stimulated RAW 264.7 cells by blocking NF-κ B/Rel and AP-1 activation. Immunopharmacology 48:173-183   DOI
23 Jin SY, Sim KH, Lee EJ, Gu HJ, Kim MH, Han YS, Park JS, Kim YH. 2014. Changes in quality characteristics and antioxidant activity of apples during storage. Korean J Food Nutr 27:999-1005   DOI
24 Kang IK, Lee GJ, Kim MJ, Kwon SI, Peak PY, Choi DG. 2002. Selection of crabapple as pollinizers for major apple cultivars in apple orchard. Hortic Sci Technol 20:330-334
25 Williams RR, Church RM. 1983. Growth and flowering of ornamental Malus pollinators in apple orchards. J Hortic Sci 58:337-342   DOI
26 Kang IK. 2004. Selection of crab apples as pollinizers for 'Hongro' apple cultivar. Hortic Sci Technol 22:212-215
27 Kim GH, Lee EJ, Ryu SM, Sohn HY, Kim JS. 2021. Anti-inflammatory activities of apple extracts and phloretin. J Life Sci 31:158-163   DOI
28 Lee YY, Kang SA. 2020. Antioxidative and anti-inflammatory activities of Salvia plebeia R. Br extracts. Korean J Food Nutr 33:483-492   DOI
29 Bang HY, Cho SD, Kim D, Kim GH. 2015. Comparison of antioxidative activities of Fuji apples parts according to production region. J Korean Soc Food Sci Nutr 44:557-563   DOI