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http://dx.doi.org/10.9799/ksfan.2021.34.5.441

Immuno-Enhancing Effects through Macrophages of Polysaccharides Isolated from Citrus Peels  

Lee, Kyung-Ae (Dept. of Food and Nutrition, Anyang University)
Park, Hye-Ryung (Graduate School of Biotechnology, and College of Life Science, Kyung Hee University)
Publication Information
The Korean Journal of Food And Nutrition / v.34, no.5, 2021 , pp. 441-448 More about this Journal
Abstract
This study was designed to investigate the intracellular signaling pathways and immunoenhancing effect of macrophage activation by crude polysaccharides (CPP) extracted from citrus peels. CPP did not affect the cytotoxicity of RAW264.7 cells, but showed dose-dependent effects on cell viability. Also, CPP showed high production of chemokine (nitric oxide (NO)) and cytokines (interleukin (IL)-6 and tumor necrosis factor (TNF)-α). CPP increased IL-6, TNF-α, and inducible nitric oxide synthase (iNOS) mRNA expression dose-dependently. CPP also strongly induced the phosphorylation of the ERK, p38, and IκBα pathways in RAW 264.7 cells. In anti-pattern recognition receptors (PRRs) experiments, the effect of CPP on NO production was strongly suppressed by neutralizing toll-like receptor (TLR)2, TLR4, and Dectin1 antibodies, whereas IL-6 and TNF-α production by CPP was mainly suppressed by mannose receptor (MR). Therefore, these results suggest that CPP treatment-induced NO production was regulated by the ERK, p38, and NF-κB pathways through TLR2, TLR4, and Dectin1 receptors, whereas IL-6 and TNF-α production was primarily regulated by the ERK, p38, and NF-κB pathways through MR receptors.
Keywords
citrus peel; polysaccharide; macrophage; signaling pathway;
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