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http://dx.doi.org/10.9799/ksfan.2021.34.1.036

The Physiological Activity of Crude Polysaccharide Solvent Extracted from Herbal Medicine Mixture  

Shin, Hyun-Young (Dept. of Integrated Biomedical and Life Science, Korea University)
Kim, Hoon (Skin Biotechnology Center, Kyung Hee University)
Shin, Ji-Young (Cosis-Bio Co. Ltd.)
Lee, Sue Jung (Major in Food Science and Biotechnology, Kyonggi University)
Yu, Kwang-Won (Major in Food and Nutrition, Korea National University of Transportation)
Publication Information
The Korean Journal of Food And Nutrition / v.34, no.1, 2021 , pp. 36-46 More about this Journal
Abstract
After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.
Keywords
herbal medicine; solvent extract; anti-oxidant activity; anti-inflammatory activity; macrophage-stimulating activity;
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Times Cited By KSCI : 2  (Citation Analysis)
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