Browse > Article
http://dx.doi.org/10.9799/ksfan.2019.32.5.408

Anti-Inflammatory Effects of Beopje Processed Curly Dock (Rumex crispus L.) in LPS-Induced Murine RAW 264.7 Cell Lines  

Kim, Seung-Hee (Dept. of Convergence Science and Technology, Graduate School of Venture, Hoseo University)
Kang, Soon Ah (Dept. of Convergence Science and Technology, Graduate School of Venture, Hoseo University)
Publication Information
The Korean Journal of Food And Nutrition / v.32, no.5, 2019 , pp. 408-416 More about this Journal
Abstract
This study investigated the anti-inflammatory effects of processed (Beopje) curly dock (Rumex crispus L.) in LPS (lipopolysaccharide)-stimulated murine RAW 264.7 cells. The experimental group was classified into five groups : LPS no treatment, CD (curly dock), CD-B (CD processed through Beopje), LPS, LPS+CD-B (LPS+CD processed through Beopje) and LPS+CD (LPS+CD). Treatment of the Raw 264.7 cell lines using LPS led to a significant increase in NO production, pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$), and inflammation related genes (COX-2 and iNOS). Investigation of the inhibitory effects of CD and processed CD on NO production and expression of iNOS and COX-2 was done in LPS-induced RAW 264.7 cells. There was significant inhibition of NO production by LPS+CD and LPS+CD-B in a dose-dependent manner (p<0.05). Particularly, LPS+CD-B exhibited reduced mRNA expression of iNOS and COX-2 and NO production as compared to LPS+CD in Raw 264.7 cell lines (p<0.05). These results may explain some known biological activities of curly dock including the anti-inflammatory effects. CD-B in particular exhibited the highest anti-inflammatory effects of inhibiting production of NO, through the regulation of inflammatory related genes and pro-inflammatory cytokines. These results of Beopje processing might help decrease the anti-biological effects and increase several active substances of curly dock.
Keywords
curley dock; LPS; Beopje processed; anti-inflammatory effect;
Citations & Related Records
Times Cited By KSCI : 11  (Citation Analysis)
연도 인용수 순위
1 Kim SJ, Kim YG, Park KY. 2012. Inhibitory effects of ginger and processed (Beopje) ginger extracts on HCl-ethanol induced gastritis in rats. J Korean Soc Food Sci Nutr 41:1528-1533   DOI
2 Kim SJ. 2012. Preventive effects of Beopje ginger and taemyeongcheong on in vivo gastritis and colitis. Master's Thesis, Pusan National Univ. Pusan. Korea
3 Kim YS, Suh HJ, Park S. 2013. Antioxidant and photoprotective activities of various extracts from the roots of Rumex crispus L. Korean J Food Preserv 20:684-690   DOI
4 Lee JY, Jun DY, Yoon YH, Ko JY, Woo KS, Woo MH, Kim YH. 2014. Anti-inflammatory effect of flavonoids Kaempferol and Biochanin A-enriched extract of barnyard millet (Echinochloa crus-galli var. frumentacea) grains in LPS-stimulated RAW264.7 cells. J Life Sci 24:1157-1167   DOI
5 Lee KH, Rhee KH. 2015. Screening of anti-inflammatory herbs having the activation of MAPK family proteins. Korean J Food Nutr 28:343-350   DOI
6 Lee KM, Kang BS, Lee HL, Son SJ, Hwang SH, Kim DS, Cho HJ. 2004. Spinal NF-kB activation induces COX-2 upregulation and contributes to inflammatory pain hypersensitivity. Eur J Neurosci 19:3375-3381   DOI
7 Lee SS, Kim DH, Yim DS, Lee SY. 2007. Anti-inflammatory, analgesic and hepatoprotective effect of semen of Rumex crispus. Korean J Pharmacogn 38:334-338
8 Lee YM, Kim JS. 2003. Studies on the processing of herbal medicines (VI) - HPLC analysis of standard compounds of unprocessed-and processed herbal medicines. Korean J Orient Med 9:69-72
9 Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the $2^{-{\Delta}{\Delta}CT}$ method. Methods 25:402-408   DOI
10 Lu YC, Yeh WC, Ohashi PS. 2008. LPS/TLR4 signal transduction pathway. Cytokine 42:145-151   DOI
11 Park JA, Choi MO. 2011b. Antimicrobial activity and antiinflammation effect to the human skin pathogens by the Rumex crispus L. root extracts. Korean J Aesthe Cosmetol 9:9-16
12 Miyasaka N, Hirata Y. 1997. Nitric oxide and inflammatory arthritis. Life Sci 61:2073-2081   DOI
13 Oh DH. 1986. Studies on the quality of cures meat in the processing. Ph.D. Thesis, Chonbuk National Univ. Jeonju. Korea
14 Park DH, Jeong GT, Lee GM. 2006. Study of antimicrobial and antioxidant activities of Rumex crispus extract. Korean Chem Engine Res 44:81-86
15 Park ES, Song GH, Lee SM, Kim TY, Park KY. 2018. Increased anti-inflammatory effects of processed curly dock (Rumex crispus L.) in ex vivo LPS-induced mice splenocytes. J Korean Soc Food Sci Nutr 47:599-604   DOI
16 Park JA, Choi MO. 2011a. Antioxidant effects of Rumex crispus L. root extracts and protective effects on human HaCaT keratinocyte. J Korean Soc Cosmetol 17:263-268
17 Park SJ, Choi JH, Jung YS, Yu MH. 2013. Inhibitory effect of Rumex crispus L. fraction on adipocyte differentiation in 3T3-L1 cells. Korean J Food Sci Technol 45:90-96   DOI
18 Rao TS, Currie JL, Shaffer AF, Isakson PC. 1993. Comparative evaluation of arachidonic acid (AA)- and tetradecanoylphorbol acetate (TPA)- induced dermal inflammation. Inflammation 17:723-741   DOI
19 Rhim TJ, Choi MY, Park HJ. 2012. Antioxidative activity of Rumex crispus L. extract. Korean J Plant Res 25:568-577   DOI
20 Seo WG, Pae HO, Oh GS, Chai KY, Yun YG, Kwon TO, Chung HT. 2000. Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-gamma and lipopolysaccharide. Gen Pharmacol 35:21-28   DOI
21 Song GH, Park ES, Lee SM, Kim TY, Park KY. 2017. An atopic preventive drink (APD) reduces Th2 cytokines in LPS-treated Raw 264.7 cells. Tang 7:e15
22 Seo WG, Pae HO, Oh GS, Kim NY, Kwon TO, Shin MK, Chai KY, Chung HT. 2001. The aqueous extract of Rhodiola sachalinensis root enhances the expression of inducible nitric oxide synthase gene in RAW264.7 macrophages. J Ethnopharmacol 76:119-123   DOI
23 Shin CH. 2001. Studies on the antioxidative character in the ethyl acetate extractions of Rumex crispus. Korean J Biotechnol Bioeng 16:592-602
24 Shiwani S, Singh NK, Wang MH. 2012. Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus. Nutr Res Pract 6:389-395   DOI
25 Vlassara H, Striker LJ, Teichberg S, Fuh H, Li YM, Steffs M. 1994. Advanced glycation end products induce glomerular sclerosis and albuminuria in normal rats. Proc Natl Acad Sci USA 91:11704-11708   DOI
26 Wang Y, Vodovotz Y, Kim PK, Zamora R, Billiar TR. 2002. Mechanisms of hepatoprotection by nitric oxide. Ann NY Acad Sci 963:415-422
27 Xie QW, Kashiwabara Y, Nathan C. 1994. Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. J Biol Chem 269:4705-4708   DOI
28 Yun IJ. 2009. Inhibitory effects of methanol extract of Rumex crispus on NO production and iNOS expression from LPS-stimulated Murine RAW 264.7 cell lines. Master's Thesis, Chosun Univ. Gwangju. Korea
29 Yun YS. 2016. Physicochemical properties and antioxidative activities of Rumex crispus extract. Master's Thesis, Tongmyong Univ. Busan. Korea
30 Ahn JY, Im LR, Kim JH, Park JH, Kim DK, Lee YM. 2009. Effects of Rumecis radix water extract on development of atopic dermatitis in BALB/c mice. Korean J Pharmacogn 40:218-223
31 Amrouche T, Boutin Y, Prioult G, Fliss I. 2006. Effects of bifidobacterial cytoplasm, cell wall and exopolysaccharide on mouse lymphocyte proliferation and cytokine production. Int Dairy J 16:70-80   DOI
32 Chang SW, Kim IH, Han TJ. 1999. Anthraquinone productivity by the cultures of adventitious roots and hairy from curled dock (Rumex crispus). Korean J Plant Tissue Cult 26:7-14
33 Choi WC, Lee JH, Lee EO, Lee HJ, Yoon SW, Ahn KS, Kim SH. 2006. Study on antiangiogenic and antitumor activities of processed Rhus verniciflua Stokes extract. J Physiol Pathol Korean Med 20:825-829
34 Jeong GT, Lee GM, Park DH. 2006. Study of antimicrobial and antioxidant activities of Rumex crispus extract. Korean Chem Eng Res 44:81-86
35 Guzik TJ, Korbut R, Adamek-Guzik T. 2003. Nitric oxide and superoxide in inflammation and immune regulation. J Physiol Pharmacol 54:469-487
36 Hotamisligil GS. 2017. Inflammation, metaflammation and immunometabolic disorders. Nature 542:177-185   DOI
37 Hwang SW, Ha TJ, Lee JR, Lee J, Nam SH, Park KH, Yang MS. 2004. Isolation of anthraquinone derivatives from the root of Rumex japonicus H. J Korean Soc Appl Biol Chem 47:274-278
38 Kim AJ, Lee H, Ko HW, Ko SH, Woo N. 2016. Physiological activity of coffee beans and roasted black beans (Rhynchosia nulubilis) mixture extracts for coffee alternative beverage development. Korean J Food Nutr 29:178-185   DOI
39 Jeong KS. 2012. A study on antioxidant activity of ethanol extract from Rumex crispus and metal adsorptivity of it's root. J Korea Acad-Ind Coop Soc 13:934-940   DOI
40 Khattab RY, Arntfield SD. 2009. Nutritional quality of legume seeds as affected by some physical treatments 2. Antinutritional factors. LWT-Food Sci Technol 42:1113-1118   DOI
41 Kim DK, Choi SU, Rye SY, Lee KR. 1998. Cytotoxic constituents of Rumex crispus. Yakhak Hoeji 42:233-237
42 Kim JS, Kim HJ, Ma JY, Kim JM. 2002b. Studies on the processing of herbal medicines (II) - HPLC analysis of standard compounds of unprocessed- and processed herbal medicines. Korean J Pharmacogn 33:305-307
43 Kim HC. 2001. Study on the Standardization of Drug Processing in Traditional Korean Medicine. pp.3-56. Ministry of Health and Welfare
44 Kim HJ, Hwang EY, Im NK, Park SK, Lee IS. 2010. Antioxidant activities of Rumex crispus extracts and effects on quality characteristics of seasoned pork. Korean J Food Sci Technol 42:445-451
45 Kim JS, Kim HJ, Ko JH. 2002a. Studies on the processing of herbal medicines (III) - HPLC analysis of magnolol and inhibitory effects on the formation of advanced glycation endproducts (AGEs) in vitro of unprocessed- and processed magnolia bark. Korean J Pharmacogn 33:308-311
46 Kim SJ, Kim SH, Lim YI, Kim YG, Park KY. 2014. Inhibitory effects of ginger and Beopje ginger on DSS-induced colitis in mice. J Korean Soc Food Sci Nutr 43:477-484   DOI