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http://dx.doi.org/10.9799/ksfan.2019.32.2.148

Validation of Simultaneous Analysis Method of Standard Compounds in Fermented Kalopanax pictus Nakai by Bioconversion  

Jang, Won Hui (R&D Center, STR Biotech Co. Ltd.)
Lee, Wha Young (R&D Center, STR Biotech Co. Ltd.)
Lee, Bong Jin (R&D Center, STR Biotech Co. Ltd.)
Kim, Jean Man (R&D Center, STR Biotech Co. Ltd.)
Park, Seon Ju (R&D Center, STR Biotech Co. Ltd.)
Publication Information
The Korean Journal of Food And Nutrition / v.32, no.2, 2019 , pp. 148-154 More about this Journal
Abstract
The aim of this study was to select compounds for the standardization of fermented Kalopanax pictus Nakai (KP-F), to develop the analysis method using HPLC-PDA and to perform method validation. KP-F is a fermented powder developed to improve the original physiological activities and create a new functionality. Eleutheroside E, Acanthoside B, and Syringaresinol were selected as the standard compounds and developed our own method for simultaneous analysis. The analyte was isolated using C18 column with a gradient elution of 0.05 M phosphoric acid in water and methanol as the mobile phase at a flow rate of 1 mL/min and detected at 210 nm. As a result, all standard compounds showed good linearity with an $R^2$ (coefficient of correlation) of 1.000 and for the limit of detection range of $0.710{\sim}0.831{\mu}g/mL$, and the limit of quantification as $2.150{\sim}2.520{\mu}g/mL$. The precision was RSD (%) of less than 4.80%, while the accuracy was 4.70%>RSD (%) for the range 102.44~110.48%. In conclusion, the developed analysis method is suitable for the detection of Eleutheroside E, Acanthoside B, and Syringaresinol in KP-F.
Keywords
Kalopanax pictus; eleutheroside E; acanthoside B; syringaresinol; method validation;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
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