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http://dx.doi.org/10.9799/ksfan.2017.30.5.900

The Body Fat-lowering Effect of Garlic Powder in Peroxisome Proliferator-activated Receptor γ Coactivator-1α (PGC-1α)-luciferase Transgenic Mice  

Lee, Mak-Soon (Dept. of Nutritional Science and Food Management, Ewha Womans University)
Kim, Yangha (Dept. of Nutritional Science and Food Management, Ewha Womans University)
Publication Information
The Korean Journal of Food And Nutrition / v.30, no.5, 2017 , pp. 900-907 More about this Journal
Abstract
This study was performed to investigate the body fat-lowering effect of garlic powder in peroxisome proliferator-activated receptor ${\gamma}$ coactivator-$1{\alpha}$(PGC-$1{\alpha}$)-luciferase transgenic mice (TG). In this study, we generated transgenic mice with a PGC-$1{\alpha}$ promoter (-970/+412 bp) containing luciferase as a reporter gene. Mice were fed a 45% high-fat diet for 8 weeks to induce obesity. Subsequently, mice were maintained on either a high-fat control diet (CON), or high-fat diets supplemented with 2% (GP2) or 5% (GP5) garlic powder for an additional 8 weeks. Dietary garlic powder reduced the body weight in the GP2 and GP5 groups, compared to the CON group. Furthermore, garlic supplementation significantly decreased the plasma levels of triglycerides, total cholesterol, and leptin in the GP5 group, compared to the CON group. Specifically, luciferase activity in liver, white adipose tissue (WAT), and brown adipose tissue (BAT) was increased by garlic supplementation in a dose-dependent manner. These results suggest that the body fat-lowering effect of garlic powder might be related to PGC-$1{\alpha}$ by the increase in luciferase activity in liver, WAT, and BAT. Furthermore, transgenic mice might be useful for evaluating the body fat-lowering effect of various health functional foods.
Keywords
PGC-$1{\alpha}$; transgenic mice; garlic powder; body fat-lowering effect;
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1 Finck BN, Kelly DP. 2006. PGC-1 coactivators: inducible regulators of energy metabolism in health and disease. J Clin Invest 116:615-622   DOI
2 Han CY, Ki SH, Kim YW, Noh K, Lee da Y, Kang B, Ryu JH, Jeon R, Kim EH, Hwang SJ, Kim SG. 2011. Ajoene, a stable garlic by-product, inhibits high fat diet-induced hepatic steatosis and oxidative injury through LKB1-dependent AMPK activation. Antioxid Redox Signal 14:187-202   DOI
3 Haslam DW, James WP. 2005. Obesity. Lancet 366:1197-1209   DOI
4 Yang JY, Della-Fera MA, Nelson-Dooley C, Baile CA. 2006. Molecular mechanisms of apoptosis induced by ajoene in 3T3-L1 adipocytes. Obesity 14:388-397   DOI
5 Jalal R, Bagheri SM, Moghimi A, Rasuli MB. 2007. Hypoglycemic effect of aqueous shallot and garlic extracts in rats with fructose-induced insulin resistance. J Clin Biochem Nutr 41:218-223   DOI
6 Jisawa H, Suma K, Origuchi K, Kumagai H, Seki T, Ariga T. 2008. Biological and chemical stability of garlic-derived allicin. J Agric Food Chem 56:4229-4235   DOI
7 Kang SA, Shin HJ, Jang KH, Choi SE, Yoon KA, Kim JS. 2006. Effect of garlic on serum lipids profiles and leptin in rats fed high fat diet. J Food Sci Nutr 11:48-53
8 Keophiphath M, Priem F, Jacquemond-Collet I, Clement K, Lacasa D. 2009. 1,2-Vinyldithiin from garlic inhibits differentiation and inflammation of human preadipocytes. J Nutr 139:2055-2060   DOI
9 Kim ES, Chun HJ, Kim BK, Rhee KC. 1997. Garlic and cancer prevention. J Food Sci Nutr 2:180-190
10 Kim YS, Park KS, Kyung KH, Shim ST, Kim HK. 1996. Antibacterial activity of garlic extract against Escherichia coli. Kor J Food Sci Technol 28:730-735
11 Knutti D, Kralli A. 2001. PGC-1, a versatile coactivator. Trends Endocrinol Metab 12:360-365   DOI
12 Boudewijn Klop, Jan Willem F. Elte, Manuel Castro Cabezas. 2013. Dyslipidemia in obesity: Mechanisms and potential targets. Nutrients 5:1218-1240   DOI
13 Agarwal KC. 1996. Therapeutic actions of garlic constituents. Med Res Rev 16:111-124   DOI
14 Andersson U, Scarpulla RC. 2001. PGC-1-related coactivator, a novel, serum-inducible coactivator of nuclear respiratory factor-1-dependent transcription in mammalian cells. Mol Cell Biol 21:3738-3749   DOI
15 Block E. 1985. The chemistry of garlic and onions. Sci Am 252:114-119
16 Brennan AM, Mantzoros CS. 2006. Drug insight: the role of leptin in human physiology and pathophysiology emerging clinical applications. Nat Clin Pract Endocrinol Metab 2: 318-327   DOI
17 Chi MS. 1982. Effects of garlic products on lipid metabolism in cholesterol-fed rats. Proc Soc Exp Biol Med 171:174-178   DOI
18 Wu Z, Puigserver P, Andersson U, Zhang C, Adelmant G, Mootha V, Troy A, Cinti S, Lowell B, Scarpulla RC, Spiegelman BM. 1999. Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell 98:115-124   DOI
19 Qureshi AA, Abuirmeileh N, Din ZZ, Elson CE, Burger WC 1983. Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic. Lipids 18:343-348   DOI
20 The Korea National Health and Nutrition Examination Survey (KNHANES). 2014. Available from: http://www.cdc.go.kr/CDC/contents/CdcKrContentLink.jsp?fid=21&cid=65846&ctype=1 [cited 6 Oct 2015]
21 Meisenheimer PL, O'Brien MA, Cali JJ. 2008. Luminogenic enzyme substrates: The basis for a new paradigm in assay design. Promega Notes 100:22-26
22 Korea Food and Drug Administration (KFDA). 2004. Guideline for Functionality Evaluation of Health Functional Food 1st ed. pp.401
23 Kumar TR, Larson M, Wang H, McDermott J, Bronshteyn I. 2009. Transgenic mouse technology: principles and methods. Methods Mol Biol 590:335-362
24 Lee HS, Lim WC, Lee SJ, Lee SH, Lee JH, Cho HY. 2016. Antiobesity effect of garlic extract fermented by lactobacillus plantarum BL2 in diet-induced obese mice. J Med Food 19:823-829   DOI
25 Lee MS, Kim IH, Kim CT, Kim Y. 2011. Reduction of body weight by dietary garlic is associated with an increase in uncoupling protein mRNA expression and activation of AMPactivated protein kinase in diet-induced obese mice. J Nutr 141:1947-1953   DOI
26 Lim SW, Kim TH. 1997. Physiological activity of alliin and ethanol extacts from Korean garlic (Allium sativum L.). Kor J Food Sci Technol 29:348-354
27 Pagliei B, Aquilano K, Baldelli S, Ciriolo MR. 2013. Garlicderived diallyl disulfide modulates peroxisome proliferator activated receptor gamma co-activator 1 alpha in neuroblastoma cells. Biochem Pharmacol 85:335-344   DOI
28 Pintana H, Sripetchwandee J, Supakul L, Apaijai N, Chattipakorn N, Chattipakorn S. 2014. Garlic extract attenuates brain mitochondrial dysfunction and cognitive deficit in obese-insulin resistant rats. Appl Physiol Nutr Metab 39: 1373-1379   DOI
29 Puigserver P, Spiegelman BM. 2003. Peroxisome proliferatoractivated receptor-${\gamma}$ coactivator $1{\alpha}$ (PGC-$1{\alpha}$): transcriptional coactivator and metabolic regulator. Endocr Rev 24: 78-90   DOI