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http://dx.doi.org/10.9799/ksfan.2016.29.4.474

Vitamin C Quantification of Korean Momordica charantia by Cultivar, Harvest Time, and Maturity  

Jeong, Yun Sook (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Lee, Sang Hoon (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Song, Jin (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Hwang, Kyung-A (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Noh, Geon Min (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Hwang, In Guk (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
Publication Information
The Korean Journal of Food And Nutrition / v.29, no.4, 2016 , pp. 474-479 More about this Journal
Abstract
This study aimed to investigate the concentration of vitamin C in Momordica charantia (MC) by cultivar, harvest time, and maturity. The methods for determining vitamin C levels were validated by measuring their linearity, specificity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using HPLC. Results showed high linearity in the calibration curve, with a coefficient of correlation ($R^2$) of 0.9994. The LOD and LOQ values for vitamin C were 0.05 and $0.16{\mu}g/mL$, respectively. The relative standard deviations (RSDs) for intra- and inter-day precision of vitamin C measurements were 2.34 and 1.34%, respectively. Depending on cultivar, the concentration of vitamin C in MC varied from 20.75~107.31 mg/100 g, fresh weight, with an average level $68.85{\pm}25.57mg/100g$, FW. When MC was analyzed by harvest time, the 20150612 MC showed the highest amount of vitamin C ($113.20{\pm}1.89mg/100g$, FW). On the other hand, the highest vitamin C content by maturity was $48.59{\pm}0.87mg/100g$, FW (15 day old MC). This information on the comparative vitamin C levels of MC might be useful to food scientists and should be explored for functional food development.
Keywords
Momordica charantia; vitamin C; validation;
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Times Cited By KSCI : 14  (Citation Analysis)
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1 Ahn MJ, Yuk HJ, Lee HY, Hwang CE, Jeong YS, Hong SY, Kwon OK, Kang SS, Kim HR, Park DS, Cho KM. 2015. Effect of the enhanced biological activities and reduced bitter taste of bitter melon (Momordica charantia L.) by roasting. J Agric Life Sci 49:107-119   DOI
2 Bae SJ. 2002. The effects on antimicrobial and anticarcinogenic activity of Momordica charantia L. Korean J Nutr 35:880-885
3 Boo HO, Lee HH, Lee JW, Hwang SJ, Park SU. 2009. Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean J Medicinal Crop Sci 17: 15-20
4 Hong JY. Nam HS, Kim NW, Shin SR. 2006. Changes on the components of Elaeagnus multiflora fruits during maturation. Korean J Food Preserv 13:228-233
5 Jeong JH, Lee SH, Hue JJ, Lee KN, Nam SY, Yun YW, Jeong SW, Lee YH, Lee BJ. 2008. Effect of bitter melon (Momordica charantia) on anti-diabetic activity in C57BL/6J db/db mice. Korean J Vet Res 48:327-336
6 Kim BK, Hong JS, Yoon HJ, Hong SD, Hong SP, Lee JI. 2013. Influence of bitter melon extraction on oral squamous cell carcinoma. Kor J Oral Maxillofac Pathol 37:59-65
7 Kim JK, Lee WY. 2002. Changes of chemical components in persimmon leaves (Diospyros kaki Thunberg) during growth. J East Asian Soc Dietary Life 12:32-37
8 Kim KH, Choi SH. 2006. The coating of vitamin C on the surface of polymethylmethacrylate microsphere. Analytical Science & Technology 19:468-472
9 Kim KY, Lee JW. 2001. Quality factors and effect of cultivation environment on the quality of fruit vegetables. Kor J Hort Sci & Technol 19:197-203
10 Kim MW. 2013. Effect of bitter melon on plasma blood glucose and cholesterol levels in streptozotocin induced diabetic rats. J East Asian Soc Dietary Life 23:704-712
11 Lee HJ, Moon JH, Lee WM, Lee SG, Kim AK, Woo WH, Park DK. 2012. Charantin contents and fruit characteristic of bitter gourd (Momordica charantia L.) accessions. J Bio Enviro Control 24:379-384
12 Moon SL, Choi SH. 2014. Characteristic of cookies quality containing bitter melon (Momordica charantia L.) powder. Korean J Culinary Research 20:80-90
13 Lee JW, Lee HS, Na YW, Kang MJ, Jeon YA, Sung JS, Ma KH, Lee SY. 2015. Investigation of physicochemical properties according to different parts and maturity of Momordica charantia L. Korean J Plant Res 28:382-390   DOI
14 Lee YE. 2005. Bioactive compounds in vegetables: Their role in the prevention of disease. Korean J Food Cookery Sci 21: 380-398
15 Moon GS, Ryu BM, Lee MJ. 2003. Components and antioxidative activities of Buchu (Chinese chives) harvested at different times. Korean J Food Sci Technol 35:493-498
16 Mozafar A. 1994. Plant Vitamins: Agronomic, Physiological, and Nutritional Aspects. CRC Press, Inc. USA.
17 Nam SW, Kim MR. 2015. A Study on inhibitory activities on carbohydrase and anti-inflammatory activities of hot-water and ethanol extracts from immature dried bitter melon (Momordica charantia L.). J East Asian Soc Dietary Life 25:999-1006   DOI
18 Park HS, Kim WK, Kim HP, Yoon YG. 2015. The efficacy of lowering blood glucose levels using the extracts of fermented bitter melon in the diabetic mice. J Appl Biol Chem 58: 259-265   DOI
19 Park Y, Boo HO, Park YL, Cho DH, Lee HH. 2007. Antioxidant activity of Momordica charantia L. extracts. Korean J Medicinal Crop Sci 15:56-61
20 RDA. 2011. Food Composition Table. 8th ed. Rural development administration. Suwon. 122-222.
21 Shewfelt RL. 1990. Source of variation in the nutrient content of agricultural commodities from the farm to the consumer. J Food Qual 13:37-54   DOI
22 Yang EM, Park KW, Kang HM. 2007. Comparison of storability of fresh parsley grown in different seasons in MA storage. Journal of Bio-environment Control 16:67-71