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http://dx.doi.org/10.9799/ksfan.2016.29.3.431

Immuno-Modulatory Activities of Polysaccharides separated from Chrysanthemum zawadskii var. latilobum in Macrophage Cells  

Sung, Nak-Yun (Dept. of Food Science and Technology, Kongju National University)
Park, Yoo-Young (Dept. of Food Science and Technology, Kongju National University)
Kim, Yi-Eun (Dept. of Food Science and Technology, Kongju National University)
Cho, Eun-Ji (Dept. of Food Science and Technology, Kongju National University)
Kim, Mi-Hwan (Dept. of Food Science and Technology, Kongju National University)
Ryu, Gi-Hyung (Dept. of Food Science and Technology, Kongju National University)
Byun, Eui-Hong (Dept. of Food Science and Technology, Kongju National University)
Park, Youn-Je (Dept. of Food Science and Technology, Kongju National University)
Publication Information
The Korean Journal of Food And Nutrition / v.29, no.3, 2016 , pp. 431-437 More about this Journal
Abstract
Macrophages play a pivotal role in the innate and adaptive immune systems. This study investigated the immuno-modulatory activities of polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) in macrophages. Polysaccharides from Chrysanthemum zawadskii var. latilobum were extracted by the ethanol precipitation method. RAW 264.7 mouse macrophage cell line was treated with CZPS (4 to $128{\mu}g/mL$), and there was no cytotoxicity at a dose below $32{\mu}g/mL$. The levels of nitric oxide (NO) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$ and interleukin (IL)-6, IL-$1{\beta}$) production in the CZPS treated group ($32{\mu}g/mL$) were $6.5{\pm}0.12{\mu}m$ (NO), $1252.8{\pm}79.85$ (TNF-${\alpha}$), $305.4{\pm}29.41$ (IL-6), and $683.3{\pm}59.71$ (IL-$1{\beta}$), respectively, and they were significantly increased when compared to the control group; $2.2{\pm}0.03{\mu}m$ (NO), $452.3{\pm}38.34$ (TNF-${\alpha}$), $31.7{\pm}5.75$ (IL-6), and $184.1{\pm}11.52$ (IL-$1{\beta}$). Additionally, protein expression of inducible nitric oxide synthase (iNOS) and phosphorylation of MAPKs and NF-${\kappa}B$ expression were significantly increased upon CZPS treatment. Therefore, these results indicated that polysaccharides separated from Chrysanthemum zawadskii var. latilobum (CZPS) may have a potential immunomodulatory activity in macrophages through MAPKs and NF-${\kappa}B$ signaling, and this information is useful for the development of immune enhancing adjuvant materials using a natural ingredient.
Keywords
Chrysanthemum zawadskii var. latilobum polysaccharide; immuno-modulatory activity; macrophage activation; nitric oxide production; cytokine production;
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1 Brewer MS, Ikins WG, Harbers CAAZ. 1992. TBA values, sensory characteristics, and volatiles in ground fork during long-term frozen storage: Effects of packaging. J Food Sci 57:558-563   DOI
2 Byun MW, Byun EH. 2015. Immunological synergistic effects of combined treatment with herbal preparation (HemoHIM) and red ginseng extracts. J Korean Soc Food Sci Nutr 44: 182-190   DOI
3 Cha JH, Lim EM. 2014. Effects of Gardeniae fructus on cytokines in mouse macrophage. J Korean Obstet Gynecol 27: 1-16
4 Chun KS, Surh YJ. 2004. Signal transduction pathways regulating cyclooxygenase-2 expression: Potential molecular targets for chemoprevention. Biochem Pharmacol 15:1089-1100
5 Chung HJ, Jeon IS. 2011. Antioxidative activities of methanol extracts from different parts of Chrysanthemum zawadskii. Korean J Food Preserv 18:739-745   DOI
6 Dong C, Davis RJ, Flavell RA. 2002. MAP kinases in the immune response. Annu Rev Immunol 20:55-72   DOI
7 Erwig LP, Rees AJ. 1999. Macrophage activation and programing and its role for macrophage function in glomerular inflammation. Kidney Blood Press Res 22:21-25   DOI
8 Ha ES, Hwang SH, Yu KW, Shin KS, Cho HM, Kim CH, Park WM, Yoon TJ. 2003. Immnistimulation activity of the crude polysaccharides fractionated from Elutherococcus senticosus, and its application to prevent of tumors by combination therapy with cisplatin. Yakhak Heoji 47:159-166
9 Hong HD, Cho CW, Rhee YK, Choi HD, Lee HS. 2012. Status of technology development using immune-modulating polysaccharide. Food Sci Ind 45:1-11
10 Jeong H, Sung M, Kim Y, Ham H, Choi Y, Lee J. 2012. Anti-inflammatory activity of Salvia plebeia R. Br. leaf through heme oxygenase-1 induction in LPS-stimulated RAW 264.7 macrophages. J Korean Soc Food Sci Nutr 41:888-894   DOI
11 Jeong JB, Hong SC, Jeong HJ, Koo JS. 2012. Anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino on LPS-stimulated RAW 264.7 and THP-1 cells. Korean J Plant Res 25:299-307   DOI
12 Jiang MH, Zhu L, Jiang JG. 2010. Immunoregulatory actions of polysaccharides from Chinese herbal medicine. Expert Opin Ther Tar 14:1367-1402   DOI
13 Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Sci Technol 27:978-984
14 Kim YY, Lee SY, Yim DS. 2001. Biological activities of linarin from Chrysanthemum zawadskii var. latilobum. Yakhak Heoji 6:604-610
15 Kwon HS, Ha TJ, Hwang SW, Jin YM, Nam SH, Park KH, Yang MS. 2006. Cytotoxic flavonoid from the whole plants of Chrysanthemum zawadskii var. latilobum Kitamura. J Life Sci 16:746-749   DOI
16 Lebovic DI, Bentzien F, Chao VA, Garrett EN, Meng YG, Taylor RN. 2000. Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-$1{\beta}$. Mol Hum Reprod 6:269-275   DOI
17 Lee HJ, Lee CW, Choi MS, Son DJ, Hong JT. 2006. Effects of esthetic essential oils on LPS-induced nitric oxide generation in murine macrophage RAW 264.7 cells. J Soc Cosmet Scientists Korea 32:111-116
18 Miyasaka N, Hirata Y. 1997. Nitric oxide and inflammatory arthritides. Life Sci 61:2073-2081   DOI
19 Lee SH, Lee JS. 2007. Production and characteristics of antidandruffy compound from Chrysanthemum zawadskii. Kor J Microbiol Biotechnol 35:220-225
20 Mcdaniel ML, Kwon G, Hill JR, Marshall CA, Corbett JA. 1996. Cytokines and nitric oxide in islet inflammation and diabetes. Proc Soc Exp Biol Med 211:24-32   DOI
21 Park JA, Jin KS, Kwon HJ, Kim BW. 2015. Antiobesity activity of Chrysanthemum zawadskii methanol extract. Journal of Life Science 25:299-306   DOI
22 Schmidt H, Walter U. 1994. NO a work. Cell 78:919-925   DOI
23 Wang H, Actor JK, Indrigo J, Olsen M, Dasgupta A. 2003. Asian and Siberian ginseng as a potential modulator of immune function: An in vitro cytokine study using mouse macrophage. Clin Chim Acta 327:123-128   DOI