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http://dx.doi.org/10.9799/ksfan.2015.28.1.111

3,4-Dihydroxytoluene Inhibits Epidermal Growth Factor-induced Cell Transformation in JB6 P+ Mouse Epidermal Cells by Suppressing Raf-1  

Kim, Ji An (School of Food Science and Biotechnology, Kyungpook National University)
Kim, Ji Hye (School of Food Science and Biotechnology, Kyungpook National University)
di Ruccio, Eric (School of Food Science and Biotechnology, Kyungpook National University)
Kang, Nam Joo (School of Food Science and Biotechnology, Kyungpook National University)
Publication Information
The Korean Journal of Food And Nutrition / v.28, no.1, 2015 , pp. 111-118 More about this Journal
Abstract
In summary, the rutin metabolite DHT significantly reduced EGF-induced neoplastic transformation in JB6 P+ cells. This inhibition was mediated mainly by blocking the Raf/MEK/ERK signaling pathway and subsequent suppression of AP-1 activities. DHT attenuated Raf-1 kinase activity by directly binding to Raf-1 in vitro and ex vivo. Taken together, these results suggest that Raf-1 may be a critical molecular target to suppress DHT-induced neoplastic transformation, which is mainly attributable to the chemopreventive potential of several foods containing rutin.
Keywords
DHT; EGF; Raf-1;
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