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http://dx.doi.org/10.9799/ksfan.2012.25.3.546

Effect of Cassia tora Extracts on D-Galactosamine-induced Liver Injury in Rats  

Hong, Kyung-Hee (Dept. of Food and Nutrition, Baewha Women's University)
Um, Min-Young (Division of Metabolism and Functionality Research, Korea Food Research Institute)
Ahn, Ji-Yun (Division of Metabolism and Functionality Research, Korea Food Research Institute)
Ha, Tae-Youl (Division of Metabolism and Functionality Research, Korea Food Research Institute)
Publication Information
The Korean Journal of Food And Nutrition / v.25, no.3, 2012 , pp. 546-553 More about this Journal
Abstract
This study was performed to evaluate the biological activity and protective effect of Cassia tora ethanol extracts against D-galactosamine induced hepatotoxicity in rats. Male Sprague-Dawley rats were grouped into normal group, D-galactosamine treated group(control), D-galactosamine plus 0.25% Cassia tora extracts treated group and D-galactosamine plus 0.5% Cassia tora extracts treated group. Normal and control group were fed control diet and Cassia tora extracts treated groups were fed experimental diets containing 0.25% or 0.5% Cassia tora ethanol extracts for 5 weeks. Body weight gain and liver weight of rats were not significantly different between groups. Cholesterol and triglyceride concentrations in serum and liver were significantly lower in rats treated only with D-galactosamine compared to normal group, and improved in Cassia tora extracts supplemented rats. D-galactosamine treatment significantly increased serum aspartate transaminase, alanine transaminase, gamma glutamyl transferase and alkaline phosphatase, however, the activities of aspartate transaminase and alanine transaminase were significantly decreased in Cassia tora extracts supplemented rats when compared with D-galactosamine treated control group. Cassia tora extracts significantly suppressed the D-galactosamine-induced elevation of liver thiobarbituric acid reactive substances(TBARS) contents. Superoxide dismutase activity was decreased by D-galactosamine treatment, however by the supplementation of Cassia tora ethanol extracts, significantly increased in dose-dependent manner. Glutathione peroxidase activity in rats fed diets containing Cassia tora extracts was decreased compared to control. Based on these results, we concluded that Cassia tora ethanol extracts may prevents the D-galactosamine-induced hepatotoxicity probably via an antioxidant mechanism.
Keywords
Cassia tora extract; hepatoprotection; D-galactosamine; rat;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Lettéron P, Labbe G, Degott C, Berson A, Fromenty B, Delaforge M, Larrey D, Pessayre D. 1990. Mechanism for the protective effects of silymarin against carbon tetrachloride-induced lipid peroxidation and hepatotoxicity in mice. Evidence that silymarin acts both as an inhibitor of metabolic activation and as a chain-breaking antioxidant. Biochem Pharmacol 39:2027-2034   DOI   ScienceOn
2 Lim SJ, Han HK. 1997. Hypoglycemic effect of fractions of Cassia tora extract in streptozotocin-induced diabetic rats. Korean J Soc Food Sci 13:23-29   과학기술학회마을
3 Lowry OH, Rosebrough, NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biol Chem 193:265-275
4 Marklund S, Marklund CT. 1974. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469-473   DOI   ScienceOn
5 Mates JM, Perex C, Castro I. 1999. Antioxidant enzymes and human disease. Clin Biochem 32:595-603   DOI   ScienceOn
6 Mutanen ML, Mukkanene HM. 1984. Effect of dietary fat on plasma glutathione peroxidase levels and intestinal absorption of 75Se-labeled sodium selinite in chicks. J Nutr 114:829-836   DOI
7 Na GM, Han HS, Ye SH, Kim HK. 2004. Extraction characteristics and antioxidative activity of Cassia tora L. extracts. Korean J Food Culture 19:499-505   과학기술학회마을
8 Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobartituric acid reaction. Anal Biochem 95:35-41
9 Pati UK, Saraf S, Kixit VK. 2004. Hypolipidemic activity of seeds of Cassia tora Linn. J Ethnopharmacol 90:249-252   DOI   ScienceOn
10 Singh B, Nadkarni JR, Vishwakarma RA, Bharate SB, Nivsarkar M, Anandjiwala S. 2012. The hydroalcoholic extract of Cassia alata(Linn.) leaves and its major compound rhein exhibits antiallergic activity via mast cell stabilization and lipoxygenase inhibition. J Ethnopharmacol 141:469-473   DOI   ScienceOn
11 Yuk DY, Lee MY, Yun YP. 2004. Effect of green tea catechin on acute hepatotoxicity in rats. J Fd Hyg Safety 19:105-111   과학기술학회마을
12 박종희, 이정규. 1992. 상용약용식물도감. pp.26-28. 도서출판신일산사
13 정보섭, 신민교. 1998. 도해 향약(생약)대사전(식물편). pp.813-814. 도서출판 영림사
14 Choi JS, Lee HJ, Park KY, Ha JO, Kang SS. 1997. In vitro antimutagenic effects of anthraquinone agycones and naphthopyrone glycosides from Cassia tora. Planta Med 63:11-14   DOI   ScienceOn
15 Choi HJ, Han MJ, Baek NI, Kim DH, Jung HG, Kim NJ. 2006. Hepatoprotective effects of Brassica rapa(Turnip) on Dgalactosamine induced liver injured rats. Kor J Pharmacogn 37:258-265
16 Choi HJ, Kim EJ, Han MJ, Baek NI, Kim DH, Jung HG, Kim NJ. 2007. Hepatoprotective effect of fermented Artemisia princeps Pampanini by lactic acid bacteria. Kor J Pharmacogn 38:245-253   과학기술학회마을
17 Choi JS, Lee HJ, Kang SS. 1994. Alatermin, cassiaside and rubrofusarin gentiobioside, radical scavenging principles from the seeds of Cassia tora on DPPH radical. Arch Phar Res 17:462-466   DOI
18 Decker K, Keppler D, Pausch J. 1973. The regulation of pyrimidine nucleotide level and its role in experimental hepatitis. Adv Enzyme Regul 11:205-230   DOI   ScienceOn
19 Farber JL, Gill G, Konishi Y. 1973. Prevention of galactosamineinduced liver cell necrosis by uridine. Am J Pathol 72:53-62
20 Folch J, Lees M, Sloane GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497-509
21 Ha TY, Cho IJ, Lee HY. 2001b. Effect of Cassia tora ethanol extracts on carbon tetrechloride-induced liver injury rats. Korean J Food Sci Technol 33:789-794   과학기술학회마을
22 Ha TY, Cho IJ, Seong KS, Lee SH. 2001a. Effect of Cassia tora ethanol extract on the lipid levels of serum liver in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 30:1171-1176   과학기술학회마을
23 Hayes AW. 1982. Principles and Methods of Toxicology. pp. 407-445. Gabriel L. Plaa and Willian R. Hewitt, Raben Press, New York
24 Kim KH, Han HK. 1998. The effect of mushroom extracts on carbon tetrachloride-induced hepatotoxicity in rats. J Korean Soc Food Sci Nutr 27:326-332   과학기술학회마을
25 Im SJ, Lee SH, Lee HS, Park MJ. 2005. Antioxidative activity of some natural products which have been orientally used as ophthalmic drugs. J Korean Oph Opt Soc 10:365-373
26 Jang DJ, Joo HK, Cho YJ. 1989. The protective effect of the seeds of Cassia tora L. against carbon tetrachloride-induced hepatic injury on rats. Analytical Sci & Technol 2:331-335
27 Keppler D, Decker K. 1969. Studies on the mechanism of galactosamine-1-phosphate and its inhibition of UDP-glucose pyrophosphorylase. Eur J Biochem 10:219-225   DOI   ScienceOn
28 Kim NY, Lee JS, Park MJ, Lee KH, Kim SH, Choi JW, Park HJ. 2004. The hepatoprotective effect of active compounds of Kochiae fructus on D-galactosamine-intoxicated rats. J Korean Soc Food Sci Nutr 33:1286-1293   과학기술학회마을   DOI   ScienceOn
29 Abei H. 1974. Catalase. In "Methods of Enzymatic Analysis' Vergmeyer, M.U. (ed). Academic Press, New York. 2:673-679
30 Ahn BY. 2009. Desmutagenic effect of water extract from Cassia tora L. on the mutagenicity of N-methyl-N-nitro-N'-nitrosoguanidinein in E. coli PQ37. J Fd Hyg Safety 24:46-49   과학기술학회마을
31 Aviello G, Rowland I, Gill CI, Acquaviva AM, Capasso F, McCann M, Capasso R, Izzo AA, Borrelli F. 2010. Antiproliferative effect of rhein, an anthraquinone isolated from Cassia species, on Caco-2 human adenocarcinoma cells. J Cell Mol Med 4:2006-2014
32 Cho SH, Kim TH, Lee NH, Son HS, Cho IJ, Ha TY. 2005. Effects of Cassia tora fiber supplement on serum lipids in Korean diabetic patients. J Med Food 8:311-318   과학기술학회마을   DOI   ScienceOn