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http://dx.doi.org/10.9799/ksfan.2011.24.4.740

Effect of Ohmic Heating on Characteristics of Heating Denaturation of Soybean Protein  

Cha, Yun-Hwan (Dept. of Food and Nutrition, SoongEui Women's College)
Publication Information
The Korean Journal of Food And Nutrition / v.24, no.4, 2011 , pp. 740-745 More about this Journal
Abstract
Ohmic heating uses electric resistance heat which occurs equally and rapidly inside food when an electrical current is passed throught. In this study, we observed the physical & chemical characteristics changes which occurs in soybean protein during heating denaturation by using ohmic and conventional heating. After the ohmic heating process, we could not find any change of the primary protein structure in the denaturated soy protein samples. However, the rate of imbibed water(RIW) of the ohmic samples was 2 times faster than that of the conventional samples. Also the ANS-surface hydrophobicity was decreased, which is very closely related to RIW. In the differential scanning calorimeter(DSC) analysis result, all 7S soyprotein fraction samples were completely denaturated by ohmic and conventional heating. However, the 11S samples were completely denatured only by ohmic heating. According to the DSC result, we decided that soyprotein was damaged by temperature and electrical current during ohmic heating. The damage of electrical current was a cause of the characteristic changes.
Keywords
ohmic heating; soy protein fraction; rate of imbibed water(RIW); ANS-surface hydrophobicity; differential scanning calorimeter(DSC);
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  • Reference
1 Arrese EL, Sorgentini DA, Wagner JR, Anon WC. 1991. Electrophoretic, solubility and functional properties of commercial soy protein isolates. J Agric Food Chem 39:1029-1132   DOI
2 Byun MW, Kahg IJ, Mori T. 1995. Properties of soya milk and tofu prepared with gamma-irradiated soya beans. J Sci Food Agric 67:477-483   DOI   ScienceOn
3 De Alwis AAP, Fryer PJ. 1990. The use of direct resistance heating in the food industry. J Food Eng 11:3-27   DOI   ScienceOn
4 Keshun Liu. 1997. Soybeans - Chemistry, Technology and Utilization. Chapman & Hall. pp.1-3
5 Kim JS, Pyun YR. 1995. Extraction of soybean milk using ohmic heating. Kor Soybean Digest 12:33-38
6 Kohyama K. 1965. Purification of 7S component of soybean protein. Agr Biol Chem 39:885-888
7 Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685   DOI   ScienceOn
8 Schreier PJR, Reid DJ, Fyer PJ. 1993. Enhanced diffusion during the electrical heating of foods. Int J Food Sci Technol 22:249-260
9 Shi YG, Ren L. 1995. Soyfood Technology. China's Light Industry Publisher.
10 Sorgentini DA, Wagner JR, Anon MC. 1995. Effects of thermal treatment of soy protein isolate on the characteristics and structure-function relationship of soluble and insoluble fraction. J Agric Food Chem 43:2471-2479   DOI   ScienceOn
11 Sorgentini DA, Wagner JR, Arrese EL, Anon MC. 1991. Water imbibing capacity of soy protein isolates: Influence of protein denaturation. J Agric Food Chem 39:1386-1391   DOI
12 Thanh VH, Shibasaki K. 1976. Major proteins of soybean seeds. A straight forward fractionation and their characterization. J Agric Food Chem 24:1117-1121   DOI
13 Utsumi S, Kinsella JE. 1985. Forces involves in soy protein gelation: Effects of various reagents on the formation, hardness and solubility of heat-induced gels made from 7S, 11S and soy isolate. J Food Sci 50:1278-1282   DOI
14 Wagner JR, Anon MC. 1990. Influence of denaturation, hydrophobicity and sulfurhydryl content on solubility and water absorbing capacity of soy protein isolates. J Food Sci 55: 765-770   DOI