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The Effect of Blueberry on ROS Accumulation and Cell Death in Human Normal Breast Epithelial(MCF10A) and Breast Cancer(MCF7) Cells  

Lee, Se-Na (Dept. of Food and Nutrition, DukSung Women's University)
Kang, Keum-Jee (Dept. of Food and Nutrition, DukSung Women's University)
Publication Information
The Korean Journal of Food And Nutrition / v.21, no.4, 2008 , pp. 416-424 More about this Journal
Abstract
In an effort to elucidate the differential actions of blueberry(BB) in both normal and cancer cells, we utilized human breast cell lines to assess the accumulation of radical oxygen species(ROS) and ROS-associated apoptosis in both human normal breast epithelial(MCF10A) and breast cancer(MCF7) cells. BB extract was added to the cultures at a final concentration of $20{\mu}g/m{\ell}$ for 0(control), 6, 12, and 24 hr intervals. The MCF10A cells evidenced no marked ROS accumulation in the presence of BB, whereas the MCF7 cells evidenced clear ROS accumulation upon BB treatment from 12 hours forward. The number of dying or dead cells did not increase in the BB-treated MCF10A cell groups, whereas that number increased profoundly from 12 hr forward. Furthermore, the expression levels of certain stress-related, and pro- and antiapoptotic gene products evidenced differential responses to BB treatment between the MCF10A and MCF7 cell groups. These results indicate that the components of BB extract differentiate cancer cells by not preventing ROS accumulation within cells and by inducing ROS-associated cell death in cancer cells. However, no marked ROS accumulation or induction of cell death was noted in the normal breast epithelial cells. The fact that BB extract exerted a differential effect on cancer cells opens further directions of research regarding the specific components that exert the differential BB-mediated effects in the selective prevention of normal cells and therapy for cancer tissues in the physiological body.
Keywords
blueberry; breast cancer; ROS; cell death; MCF10A; MCF7 cell;
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1 Zhao, X, Goswami, M, Pokhriyal, N, Ma, H, Du, H, Yao, J, Victor, TA, Polyak, K, Sturgis, CD, Band, H and Band, V. Cyclooxygenase-2 expression during immortalization and breast cancer progression. Cancer Res. 68:467-475. 2008   DOI   ScienceOn
2 Knekt, P, Jarvinen, R, Seppanen, R, Heliovaara, M, Teppo, L, Pukkala, E and Aroma, A. Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. Am. J. Epidemol. 146:223-230. 1997   DOI   ScienceOn
3 Smith, MAL, Marley, KA, Seigler, DA, Singletary, KW and Meline, B. Bioactive properties of wild blueberry fruits. J. Food Sci. 65:352-356. 2000   DOI   ScienceOn
4 Hakkinen, S, Heinonen, M, Karenlampi, S, Mykkanen, H. Ruuskanen, J and Torronen, R. Screening of selected flavonoids and phenolic acids in 19 berries. Food Res. Int. 32:345-353. 1999   DOI   ScienceOn
5 Rothe, G and Valet, G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2',7'-dichlorofluorescein. J. Leukoc. Biol. 47:440-448. 1990   DOI
6 Soule, HD, Maloney, TM, Wolman, SR, Peterson, WD, Brenz, R, McGrath, CM, Russo, J, Pauley, RJ, Jones, RF and Brooks, SC. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF10. Cancer Res. 50:6075-6086. 1990
7 Leek, RD, Kallamanis, L, Pezzella, F, Gatter, KC and Harris, Al. Bcl-2 in normal human breast and carcinoma, association with estrogen receptor-negative, epidermal growth factor receptor-negative tumor and in situ cancer. Br. J. Cancer. 69:135-139. 1994   DOI   ScienceOn
8 Hope, SS, Tate, PL, Huang, G, Magee, JB, Meepagala, KM, Wedge, DE and Larcom, LL. Antimutagenic activity of berry extracts. J. Med. Food. 7:450-455. 2004   DOI   ScienceOn
9 Willis, L, Bickford, P, Zaman, V, Moore, A and Granholm, AC. Bluberry extract enhances survival of intraocular hippocampal transplants. Cell Transplant. 14:213-23. 2005   DOI   ScienceOn
10 Olsson, ME, Gustavsson, KE, Anderson SF, Nilsson A and Duan, RD. Inhibition of cancer cell proliferation in vitro by fruit and berry extracts and correlations with antioxidant levels. J. Agric. Food Chem. 52:7264-7271. 2004   DOI   ScienceOn
11 Chomczynski, P and Sacchi, N. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159. 1987
12 Benhar, M, Engelberg, D and Levitzkia, A. ROS, stressactivated kinases and stress signaling in cancer. EMBO Rep. 3:420-425. 2002   DOI   ScienceOn
13 Seeram, NP, Adams, LS, Zhang, YS, Sand, DN, Sand, DN, Scheuller, HS and Heber, DV. Blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J. Agric. Food Chem. 54:9329-9339. 2006   DOI   ScienceOn
14 Knekt, P, Jarvinen, R, Reunanen, A and Maatela, J. Flavonoid intake and coronary mortality in Finland: a cohort study. Br. Med. J. 312:478-481. 1996   DOI   ScienceOn
15 Reed, J. Bcl-2 and regulation of programmed cell death. J. Cell Biol. 124:1-6. 1994   DOI
16 Reddivari, L, Vanamala, J, Chintharlapalli, S, Safe, SH and Miller, JC. Anthocyanin fraction from potato extracts is cytotoxic to prostate cancer cells through activation of caspasedependent and caspase-independent pathways. Carcinog. 28: 2227-2235. 2007   DOI   ScienceOn
17 Neto, CC. Cranberry and blueberry: Evidence for protective effects against cancer and vascular diseases. Mol. Nutr. Food Res. 51:652-664. 2007   DOI   ScienceOn
18 Montcourrier, P, Mangeat, PH, Salazar, G, Morisset, M, Sahuquet, A and Rochefort, H. Cathepsin D in breast cancer cells can digest extracellular matrix in large acidic vesicles. Cancer Res. 50:6070-6086. 1990
19 Keli, SO, Hertog, MGL, Feskens, EJM and Kromhout, D. Dietary flavonoids, antioxidant vitamins, and incidence of stroke: the Zutphen study. Arch. Int. Med. 156:637-642. 1996   DOI
20 Rodhe, M, Daugaard, M, Jensen, MH, Helin, K, Nylandsted, J and Jaattela, M. Members of the heat-shock protein 70 family promote cancer growth by distinct mechanism. Genes Dev. 19:570-582. 2005   DOI   ScienceOn
21 Du, Y and Lou, H. Catechin and proanthocyanidin B4 from grape seeds prevent doxorubicin-induced toxicity in cardiomyocytes. Eur. J. Pharmacol. 591:96-101. 2008   DOI   ScienceOn
22 Buttke, TM and Sandstrom, PA. Oxidative stress as a mediator of apoptosis. Immunol. Today. 15:7-10. 1994   DOI   ScienceOn
23 Naczk, M and Shahidi, F. Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. J. Pharm. Biomed. Anal. 41:1523-1542. 2006   DOI   ScienceOn
24 Wedge, DE, Meepagala, KM, Magee, JB, Smith, SH, Huang, G and Larcom, LL. Anticarcinogenic activity of strawberry, blueberry, and raspberry extracts to breast and cervical cancer cells. J. Med. Food. 4:49-52. 2001   DOI   ScienceOn
25 Ahn, SJ, Bae, JY, Lee, RA, Han, WS, Kim, SW, Chae, HZ and Noh, DY. Protective role of Prx(peroxiredoxin) I and II against $H_{2}O_{2}$-induced apoptosis of MCF7 cell lines. J. Kor. Breast Cancer. 6:68-74. 2003   DOI
26 Yi, W, Fischer, J, Krewer, G and Akoh, C. Phenolic compounds from blueberries can inhibit colon cancer cell proliferation and induce apoptosis. J. Agric. Food Chem. 53: 7320-7329. 2005   DOI   ScienceOn
27 Hertog, MGL, Feskens, EJM and Kromhout, D. Antioxidant flavonols and coronary heart disease risk. Lancet. 349:699. 1997
28 Bagchi, D, Sen, CK, Bagchi, M and Atalay, M. Anti-angiogenic, antioxidant, and anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula. Biochem. 69:75-80. 2004
29 Greenlund, LJ, Deckwerth, TL and Johnson, EM. Superoxide dismutase delays neuronal apoptosis: A role for reactive oxygen species in programmed neuronal death. Neuron. 14: 303-315. 1995   DOI   ScienceOn