Browse > Article

Medium Optimization for Fibrinolytic Enzyme Production by Bacillus subtilis MG410 Isolated  

Lee Ju-Youn (Dept. of Food Science & Biotechnology, Food Science & Biotechnology Research Center, Hankyong National University)
Paek Nam-Soo (Mediogen, Co. Ltd)
Kim Young-Man (Dept. of Food Science & Biotechnology, Food Science & Biotechnology Research Center, Hankyong National University)
Publication Information
The Korean Journal of Food And Nutrition / v.18, no.1, 2005 , pp. 39-47 More about this Journal
Abstract
Using the bacteria isolated from Chungkookjang, Bacillus sublilis MG410 which is excellent in fibrinolytic enzyme activity was isolated. In increase the high production of fibrinolytic enzyme from Bacillus sublilis MG410, the effect of various carbon sources, nitrogen sources, inorganic sources, the initial pH of medium were investigated. The most effective carbon and nitrogen sources were founded cellobiose 0.5%(w/v) and soybean meal 2%(w/v) respectively. None of inorganic sources examined had any detectable stimulating effect on fibrinolytic enzyme production except Na₂HPO₄·12H₂O. The initial optimum pH for fibrinolytic enzyme production ranged from 5∼6 and agitation speed was effect at 150rpm. In jar fermentor experiments under optimal culture conditions, the activity of fibrinolytic enzyme reached about 5.050 unit after 48hours.
Keywords
Chungkookjang; Bacillus subtilis; fibrinolytic enzyme;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Harlan, JM and Harker, LA. Haemostasis, thrombosis and thromboembolic disorder. Med. Clin. North Am. 65:855-857. 1981   DOI
2 Electricwala, AR, Sawyer, T, Powell, JC and Atkinson, T. Isolation of thrombin inhibitor from the jeech Hirudinaria manillensis. Blood Coagul. Fibrin. 2:83-85. 1991   DOI   ScienceOn
3 Kembhavi, AA, Knkalni, A and Pant, A. Salt to lerant and thermostable alkaline protease from Bacillus sp. NCIM No. 64. Appl. Biochem. Biotech. 38:83-89. 1993   DOI   PUBMED
4 Lee, SK. Sohn, JH, Choi, ES and Rhee, SK. Screening and purification of anticoagulant protein from Korean lecches. Kor. J. Biochem. 26:228-234. 1993
5 Ranhman, RN, ZA, Kamaruzaman, CNR, Yunus, WM, MB, ZW and Salleh, AB. Purification and characterization of a heat-stable alkaline protease from Bacillus stearothermophilus F1. Appl. Microbiol Biotechnol. 40:822- 827. 1994   DOI   ScienceOn
6 Santos, JA, LJ, Cabral, MS and Cooney, CL. Recovery of alkaline protease by membrane filtration. Bioprocess Eng. 7:205-211. 1992   DOI
7 Sumi, H. Nattokinase and health-development' of natto. Bioindustry 7:725-730. 1990
8 Lee, SK, Heo, S and Joo, HK. Isolation and identification of fibrinolytic bacteric from Korean traditional Chungkookjang. Kor. Agr. Chem. Soc. 41 (inpress). 1998
9 Kol, JO, Kim, HK and Park, I. Production and characterization of fibrinolytic enzyme: Optimal condition for production of the enzyme from Bacillus sp. KP-6408 isolated from Chungkookjang. L. Korean Soc. Food Sci. Nutr. 27:51-56. 1997
10 Lee, EO, Park, EH, Lee HH and Hyun, HH. Isolation and characterization of Pseudomonas sp. producing alkaline protease. Kor. J. Microbiol. 22: 289-297. 1990
11 Lee, SK, Heo, S, Bae, DH and Choi, KH. Medium Optimization for Fibrinolytic Enzyme Production by Bacillus subtilis KCK-7 Isolated from Korean Traditional Chungkookjang. Kor. J. Appl. Microbiol. Biotechnol 26:226-232. 1998
12 Krstenansky, JL, Owen, TJ, Yates, MT and Man, SJT. The C-terminal binding domain of hirullin P18 antithrombin activity and comparison to hirudin peptides. FEBS Letters 269:425-4310. 1990   DOI   ScienceOn
13 Sumi, H, Hamada, H, Tsushima, H, Mihara, H and Muraki, H. A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Nattor; a typical and popular soybean food in the Japanese diet. Experimentia 43:1110-1111. 1987   DOI   PUBMED   ScienceOn
14 Koo, JH, Choi, IJ, Nam, HS, Lee, HJ, Shin, ZI and Oh, TK. Modium optimization for production of thermostable alkaline proteases from Bacillus licheniformis NS70. Kor. J. Appl. Microbiol. Biotechnol. 25:207-221. 1997
15 Takami, H, Akjba, T and Horikoshi, K. Production of extremly thermostable alkaline prutease from Bacillus sp. No. AH-101. Appl. Microbiol. Biotech. 30:120-124. 1989
16 Kim, YT. Characteristics of fibrinolytic enzyme peoduced by Bacillus sp. isolated from Chungkookiang, Sejong Univ, Doctoral Degree Thesis. 1995
17 Nakajima, N, Mihara, H and Surni. Characterization of potent fibrinolytic enzymes in earthworm. Lumbricus rubellus. Biosci. Biotech. Biothem. 57: 1730: 1737. 1993
18 Teiner, SD, Knecht, VR and Gruetter, M. Isolation and purification of novel hirudins from the leech Hirudinaria manillensis by high-performance liquid chromatography. J. Chromatograpy 530:273-279. 1990   DOI   ScienceOn
19 Lee, KH, Lee, HJ and Chung, MK. Studies on Chungkookjang(Part 1) on the changes of soybean protein in manufacturing Chungkookjang. Kor. Agr. Chrm. Soc. 14:191-196. 1971
20 Kim, KJ, Ryu MK and Kim, SS. Chungkookjang koji fermentation with rice straw. Kor. J. Food Sci. Technol. 14:301-308. 1982
21 Gomex, CC, Simoncarballo, R, Coma, AC, Sanchex de Leon, T, Montero, DE and Rodrignez, PR. The relatoionship between lipid peroxidation and platelets aggregation in atherosclerotic patients. Angiologia 43:241-246. 1991
22 Mihars, H, Sumi, H, Yoneta, T, Mizumoto, H, Ikeda, R, Seiki, M and Maruyama, M. A novel fibrinolytic enzyme extracted from the earthworm, Lumbricus fubellus. Jap. J. Physiol. 41 :461-468. 1991   DOI   ScienceOn
23 Chang, HS. Study on the alkaline prease from Xanthomonas sp. YL-37. Kon-Kuk Univ, Doctoral Degree Thesis. J 997
24 Lapentina, EG, Geep, B, Read, NG and Moncada, S. Adhesion of human platelet to collagen in the preaence of prmstacyclin, inometacin and compoihund BW775C. Thromb. Res. 37:325-330. 1986
25 Kwon, OJ, Kim, JK and Chung, YG. The characteristics of bacteria isolated from ordinary Korean soy sauce soybean paste. Kor. Agr. Chem. Soc. 29:422428. 1986
26 Markwardt, F. The isolation and chemical characterization of hirudin. Hoppe-Seylers Z. Physiol. Chem. 308:147-152. 1957   DOI
27 Kim, HK, Kim, KH, Lee, JK, Kim, YO, Nam HS and Oh, TK. Characterization of a thermostable protease from thermophilic Bacillus amyloliquefaciens NS 15-4. Kor. J. Appl. Microbiol Biotechnol. 23:322-328. 1995
28 Schinner, F and Maregensin, R. Production and properties of an extracellulae metalloprotease from a psychophilic Pseudomonas fluorescens J. Biotech. 24:207-210. 1994   DOI   ScienceOn
29 Kalebina, TS, Galina, NR, Selyakh, LO, Khodova, OM and Kulaev, IS. Serine proteinase from Bacillus brevis. Appl. Microbiol. Biotech. 28:531-533. 1988
30 Kim, WK, Kim, CY, Park, H, Choi, J, Lee, Y, Kwon, HI and Lee, S. Purification and characterizztion of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62:2482-2488. 1996
31 Horikishi, K Producrion of alkaline enxyme by aikalophilic micriirganism, Agric. Biol. Chem. 35: 1783-1791. 1971   DOI
32 Choi, KJ, Separation of Bacillus sp. and changes of NH2-N, and protease activity in Chonggukchang rmejiu adding with mufwort (Artemisia asiatica N) extract. Kon-Kuk Univ. Master's Degree Thesis. 1995