Browse > Article

Development of Antitoxic Agents from Sophora flavescens Ait.  

양희태 (대전보건대학교 식품영양과)
정승일 (원광대학교 한의학전문대학원 천연자원개발학과)
이영철 (충남대학교 의과대학 생화학교실)
윤경아 (대전 보건대학교 임상병리과)
백승화 (원광대학교 한의학전문대학원 천연자원개발학과)
Publication Information
The Korean Journal of Food And Nutrition / v.15, no.1, 2002 , pp. 36-41 More about this Journal
Abstract
The aim of this study was to assess the antitoxic compounds, flavanones(Leachianone A=LA and Sophoraflavanone G=SFG), from Sophora flavescens(S. flavescens). We investigated the possibility of antitoxicity of LA and SFG against NIH 3T3 fibroblasts cell lines using calorimetric MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl -2H-tetra-azolium bromide] assay. The results were as follows : After cadmium was treated against NIH 3T3 cell lines, we determined IC$\_$50/. Accordingly we have examined the detoxification effects of S. flavescens, LA and SFG under cadmium IC$\_$50/=12.5$\mu$M and was carried out to observe morphological changes by the light microscopic study In NIH 3T3 cells, Sophora flavescens, LA, SFG and LA + SFG showed inhibitory effects on the cytotoxicity of cadmium and these detoxication effects increased in proportion to the concentration of these drugs. These results suggest that LA and SFG from S. flavescens retain a potential antitoxic activity.
Keywords
antitoxic agent; MTT assay; Sophoraflavanone G; Leachianone A; Sophora flavescens;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Vallee, B. L. and UImer, D. D. : Biochemical effects of mercury, cadmium and lead. Ann. Rev. Biochm., 41. 91-128 (1972)   DOI   ScienceOn
2 Ryu, S. Y., Lee, H. S., Kim, Y. K., and Kim, S. H. : Determination of isoprenyl and lavanduly positions of flavonoids from Sophora flavescens by NMR Experment. Arch. pharm. Res., 20, 491-495(1997)   DOI   ScienceOn
3 Mosmann, T. : Rapid colorimetric assaya for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63(1978)   DOI   ScienceOn
4 Tang, W. and Eisenbrand, G. : Chinese Drugs of Plant Origin, Springer-Verlag, Berlin, p. 931-943(1992)
5 Williams, P. L. and Burson, J. L. : Industrial Tocicology. (eds) Van Nostrand Reinhold. p. 197-210(1985)
6 Inuma, M., Tanaka, T., Mizuno, M., Shirataki, Y., Yokoe, I., Komatsu, M and A. Lang, F. : Two flavanones in sophora leachiano and some related structure, Phytochemistry, 29(8), 2667-2669(1990)   DOI   ScienceOn
7 Foulkes, E. C.(eds) : Biological roles of metallothionein, Elsevier, New York, P, 215-222(1982)
8 Huang, M. T., Smart, R. C., Wang, C. Q., and Conney, A. H.: Inhibitory effect of carcumin, chlorogenic acid, caffeic acid, and ferulic on tumor promotion in mouse skin by 12-o-tetracecanoylphorbol-13-acetate. Cancer Res., 48, 5941-46(1988)
9 농수축산신문 : 한국식품연감, 제19장 외식산업 (1994)
10 최성인, 이정희, 이서래. : 동물실험에 의한 녹차음료의 카드뮴 및 납 제거효과, 한국식품과학회지, 26(6), 745-749 (1994)
11 국립보건원 : 식품중의 미량금속에 관한 조사연구, 29, P. 365-377 (1992)
12 Kim, Y. K., Min. B. S., and Bae, K. H. : A cytotoxic constituent from sophora flavercens, Arch. pharm. Res., 20(4), 342-345(1997)   DOI   ScienceOn
13 김운성, 이철호, 김성조, 이주돈, 문광현, 백승화.:알로에 첨가식이 흰주의 카드뮴 독성에 미치는 영향. 한국식품과학회지, 27(4), 555-563(1995)
14 Woo, E. R., Kwak, J. H., Kim, H. J., and Park, H. K. : A new prenylated prenylated flavonol from the roots of Sophora flaverscens. J. Nat. Prod., 61, 1552-1554(1998)   DOI   ScienceOn
15 Word Health Organization : Lead environmental health criteria 3. Geneva, WHO. 160(1977)
16 Beattie, J. H., Marion. M., Schmit, J. P. and Denizeau, F. : The cytotoxic effects of cadmium chloride and mercurie chloride mixtures in rat primary hepatocyte cultures, Toxicology, 62(2), 161-173(1990)   DOI   ScienceOn